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PLA-PEG Implant as a Drug Delivery System in Glaucoma Surgery: Experimental Study

Excessive postoperative scarring halts the effectiveness of glaucoma surgery and still remains a challenging problem. The purpose of this study was to develop a PLA-PEG-based drug delivery system with cyclosporine A or everolimus for wound healing modulation. Methods: PLA-PEG implants saturation wit...

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Autores principales: Germanova, Viktoriya N., Karlova, Elena V., Volova, Larisa T., Zolotarev, Andrey V., Rossinskaya, Viktoriya V., Zakharov, Ivan D., Korigodskiy, Aleksandr R., Boltovskaya, Violetta V., Nefedova, Irina F., Radaykina, Mariya V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414474/
https://www.ncbi.nlm.nih.gov/pubmed/36015676
http://dx.doi.org/10.3390/polym14163419
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author Germanova, Viktoriya N.
Karlova, Elena V.
Volova, Larisa T.
Zolotarev, Andrey V.
Rossinskaya, Viktoriya V.
Zakharov, Ivan D.
Korigodskiy, Aleksandr R.
Boltovskaya, Violetta V.
Nefedova, Irina F.
Radaykina, Mariya V.
author_facet Germanova, Viktoriya N.
Karlova, Elena V.
Volova, Larisa T.
Zolotarev, Andrey V.
Rossinskaya, Viktoriya V.
Zakharov, Ivan D.
Korigodskiy, Aleksandr R.
Boltovskaya, Violetta V.
Nefedova, Irina F.
Radaykina, Mariya V.
author_sort Germanova, Viktoriya N.
collection PubMed
description Excessive postoperative scarring halts the effectiveness of glaucoma surgery and still remains a challenging problem. The purpose of this study was to develop a PLA-PEG-based drug delivery system with cyclosporine A or everolimus for wound healing modulation. Methods: PLA-PEG implants saturation with cyclosporine A or everolimus as well as their further in vitro release were analyzed. Anti-proliferative activity and cytotoxicity of the immunosuppressants were studied in vitro using human Tenon’s fibroblasts. Thirty-six rabbits underwent glaucoma filtration surgery with the application of sham implants or samples saturated with cyclosporine A or everolimus. The follow-up period was six months. A morphological study of the surgery area was also performed at seven days, one, and six months post-op. Results: PLA-PEG implants revealed a satisfactory ability to cumulate either cyclosporine A or everolimus. The most continuous period of cyclosporine A and everolimus desorption was 7 and 13 days, respectively. Immunosuppressants demonstrated marked anti-proliferative effect regarding human Tenon’s fibroblasts without signs of cytotoxicity at concentrations provided by the implants. Application of PLA-PEG implants saturated with immunosuppressants improved in vivo glaucoma surgery outcomes. Conclusions: Prolonged delivery of either cyclosporine A or everolimus by means of PLA-PEG implants represents a promising strategy of wound healing modulation in glaucoma filtration surgery.
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spelling pubmed-94144742022-08-27 PLA-PEG Implant as a Drug Delivery System in Glaucoma Surgery: Experimental Study Germanova, Viktoriya N. Karlova, Elena V. Volova, Larisa T. Zolotarev, Andrey V. Rossinskaya, Viktoriya V. Zakharov, Ivan D. Korigodskiy, Aleksandr R. Boltovskaya, Violetta V. Nefedova, Irina F. Radaykina, Mariya V. Polymers (Basel) Article Excessive postoperative scarring halts the effectiveness of glaucoma surgery and still remains a challenging problem. The purpose of this study was to develop a PLA-PEG-based drug delivery system with cyclosporine A or everolimus for wound healing modulation. Methods: PLA-PEG implants saturation with cyclosporine A or everolimus as well as their further in vitro release were analyzed. Anti-proliferative activity and cytotoxicity of the immunosuppressants were studied in vitro using human Tenon’s fibroblasts. Thirty-six rabbits underwent glaucoma filtration surgery with the application of sham implants or samples saturated with cyclosporine A or everolimus. The follow-up period was six months. A morphological study of the surgery area was also performed at seven days, one, and six months post-op. Results: PLA-PEG implants revealed a satisfactory ability to cumulate either cyclosporine A or everolimus. The most continuous period of cyclosporine A and everolimus desorption was 7 and 13 days, respectively. Immunosuppressants demonstrated marked anti-proliferative effect regarding human Tenon’s fibroblasts without signs of cytotoxicity at concentrations provided by the implants. Application of PLA-PEG implants saturated with immunosuppressants improved in vivo glaucoma surgery outcomes. Conclusions: Prolonged delivery of either cyclosporine A or everolimus by means of PLA-PEG implants represents a promising strategy of wound healing modulation in glaucoma filtration surgery. MDPI 2022-08-21 /pmc/articles/PMC9414474/ /pubmed/36015676 http://dx.doi.org/10.3390/polym14163419 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Germanova, Viktoriya N.
Karlova, Elena V.
Volova, Larisa T.
Zolotarev, Andrey V.
Rossinskaya, Viktoriya V.
Zakharov, Ivan D.
Korigodskiy, Aleksandr R.
Boltovskaya, Violetta V.
Nefedova, Irina F.
Radaykina, Mariya V.
PLA-PEG Implant as a Drug Delivery System in Glaucoma Surgery: Experimental Study
title PLA-PEG Implant as a Drug Delivery System in Glaucoma Surgery: Experimental Study
title_full PLA-PEG Implant as a Drug Delivery System in Glaucoma Surgery: Experimental Study
title_fullStr PLA-PEG Implant as a Drug Delivery System in Glaucoma Surgery: Experimental Study
title_full_unstemmed PLA-PEG Implant as a Drug Delivery System in Glaucoma Surgery: Experimental Study
title_short PLA-PEG Implant as a Drug Delivery System in Glaucoma Surgery: Experimental Study
title_sort pla-peg implant as a drug delivery system in glaucoma surgery: experimental study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414474/
https://www.ncbi.nlm.nih.gov/pubmed/36015676
http://dx.doi.org/10.3390/polym14163419
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