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Amino-Functionalized Laponite Clay Material as a Sensor Modifier for the Electrochemical Detection of Quercetin
In this work, an electrode modified with an amino-functionalized clay mineral was used for the electrochemical analysis and quantification of quercetin (QCT). The resulting amine laponite (LaNH(2)) was used as modifier for a glassy carbon electrode (GCE). The organic–inorganic hybrid material was st...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414484/ https://www.ncbi.nlm.nih.gov/pubmed/36015934 http://dx.doi.org/10.3390/s22166173 |
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author | Ebunang, Delmas Vidal Tabe Tajeu, Kevin Yemele Pecheu, Chancellin Nkepdep Jiokeng, Sherman Lesly Zambou Tamo, Arnaud Kamdem Doench, Ingo Osorio-Madrazo, Anayancy Tonle, Ignas Kenfack Ngameni, Emmanuel |
author_facet | Ebunang, Delmas Vidal Tabe Tajeu, Kevin Yemele Pecheu, Chancellin Nkepdep Jiokeng, Sherman Lesly Zambou Tamo, Arnaud Kamdem Doench, Ingo Osorio-Madrazo, Anayancy Tonle, Ignas Kenfack Ngameni, Emmanuel |
author_sort | Ebunang, Delmas Vidal Tabe |
collection | PubMed |
description | In this work, an electrode modified with an amino-functionalized clay mineral was used for the electrochemical analysis and quantification of quercetin (QCT). The resulting amine laponite (LaNH(2)) was used as modifier for a glassy carbon electrode (GCE). The organic–inorganic hybrid material was structurally characterized using X-ray diffraction, Fourier transformed infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and CHN elemental analysis. The covalent grafting of the organosilane to the clay backbone was confirmed. The charge on the aminated laponite, both without and with the protonation of NH(2) groups, was evaluated via cyclic voltammetry. On the protonated amine (LaNH(3)(+))-modified GCE, the cyclic voltammograms for QCT showed two oxidation peaks and one reduction peak in the range of −0.2 V to 1.2 V in a phosphate buffer–ethanol mixture at pH 3. By using the differential pulse voltammetry (DPV), the modification showed an increase in the electrode performance and a strong pH dependence. The experimental conditions were optimized, with the results showing that the peak current intensity of the DPV increased linearly with the QCT concentration in the range from 2 × 10(−7) M to 2 × 10(−6) M, leading to a detection limit of 2.63 × 10(−8) M (S/N 3). The sensor selectivity was also evaluated in the presence of interfering species. Finally, the proposed aminated organoclay-modified electrode was successfully applied for the detection of QCT in human urine. The accuracy of the results achieved with the sensor was evaluated by comparing the results obtained using UV–visible spectrometry. |
format | Online Article Text |
id | pubmed-9414484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94144842022-08-27 Amino-Functionalized Laponite Clay Material as a Sensor Modifier for the Electrochemical Detection of Quercetin Ebunang, Delmas Vidal Tabe Tajeu, Kevin Yemele Pecheu, Chancellin Nkepdep Jiokeng, Sherman Lesly Zambou Tamo, Arnaud Kamdem Doench, Ingo Osorio-Madrazo, Anayancy Tonle, Ignas Kenfack Ngameni, Emmanuel Sensors (Basel) Article In this work, an electrode modified with an amino-functionalized clay mineral was used for the electrochemical analysis and quantification of quercetin (QCT). The resulting amine laponite (LaNH(2)) was used as modifier for a glassy carbon electrode (GCE). The organic–inorganic hybrid material was structurally characterized using X-ray diffraction, Fourier transformed infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and CHN elemental analysis. The covalent grafting of the organosilane to the clay backbone was confirmed. The charge on the aminated laponite, both without and with the protonation of NH(2) groups, was evaluated via cyclic voltammetry. On the protonated amine (LaNH(3)(+))-modified GCE, the cyclic voltammograms for QCT showed two oxidation peaks and one reduction peak in the range of −0.2 V to 1.2 V in a phosphate buffer–ethanol mixture at pH 3. By using the differential pulse voltammetry (DPV), the modification showed an increase in the electrode performance and a strong pH dependence. The experimental conditions were optimized, with the results showing that the peak current intensity of the DPV increased linearly with the QCT concentration in the range from 2 × 10(−7) M to 2 × 10(−6) M, leading to a detection limit of 2.63 × 10(−8) M (S/N 3). The sensor selectivity was also evaluated in the presence of interfering species. Finally, the proposed aminated organoclay-modified electrode was successfully applied for the detection of QCT in human urine. The accuracy of the results achieved with the sensor was evaluated by comparing the results obtained using UV–visible spectrometry. MDPI 2022-08-18 /pmc/articles/PMC9414484/ /pubmed/36015934 http://dx.doi.org/10.3390/s22166173 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ebunang, Delmas Vidal Tabe Tajeu, Kevin Yemele Pecheu, Chancellin Nkepdep Jiokeng, Sherman Lesly Zambou Tamo, Arnaud Kamdem Doench, Ingo Osorio-Madrazo, Anayancy Tonle, Ignas Kenfack Ngameni, Emmanuel Amino-Functionalized Laponite Clay Material as a Sensor Modifier for the Electrochemical Detection of Quercetin |
title | Amino-Functionalized Laponite Clay Material as a Sensor Modifier for the Electrochemical Detection of Quercetin |
title_full | Amino-Functionalized Laponite Clay Material as a Sensor Modifier for the Electrochemical Detection of Quercetin |
title_fullStr | Amino-Functionalized Laponite Clay Material as a Sensor Modifier for the Electrochemical Detection of Quercetin |
title_full_unstemmed | Amino-Functionalized Laponite Clay Material as a Sensor Modifier for the Electrochemical Detection of Quercetin |
title_short | Amino-Functionalized Laponite Clay Material as a Sensor Modifier for the Electrochemical Detection of Quercetin |
title_sort | amino-functionalized laponite clay material as a sensor modifier for the electrochemical detection of quercetin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414484/ https://www.ncbi.nlm.nih.gov/pubmed/36015934 http://dx.doi.org/10.3390/s22166173 |
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