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Iron(III)–Quercetin Complexes’ Safety for MRI Cell Tracking in Cell Therapy Applications: Cytotoxic and Genotoxic Assessment

The theranostic agent iron–quercetin complex (IronQ) provides a T1-positive magnetic resonance imaging (MRI) contrast agent. The magnetically IronQ-labeled cells can be used for cell tracking and have active biological applications in promoting cell and tissue regeneration. However, a detailed inves...

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Autores principales: Dechsupa, Nathupakorn, Kosintarajit, Panida, Kamkan, Kanyapak, Khanjina, Thanyalak, Sirikul, Chonticha, Innuan, Phattarawadee, Suwan, Authaphinya, Anukul, Nampeung, Kantapan, Jiraporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414527/
https://www.ncbi.nlm.nih.gov/pubmed/36014641
http://dx.doi.org/10.3390/nano12162776
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author Dechsupa, Nathupakorn
Kosintarajit, Panida
Kamkan, Kanyapak
Khanjina, Thanyalak
Sirikul, Chonticha
Innuan, Phattarawadee
Suwan, Authaphinya
Anukul, Nampeung
Kantapan, Jiraporn
author_facet Dechsupa, Nathupakorn
Kosintarajit, Panida
Kamkan, Kanyapak
Khanjina, Thanyalak
Sirikul, Chonticha
Innuan, Phattarawadee
Suwan, Authaphinya
Anukul, Nampeung
Kantapan, Jiraporn
author_sort Dechsupa, Nathupakorn
collection PubMed
description The theranostic agent iron–quercetin complex (IronQ) provides a T1-positive magnetic resonance imaging (MRI) contrast agent. The magnetically IronQ-labeled cells can be used for cell tracking and have active biological applications in promoting cell and tissue regeneration. However, a detailed investigation of IronQ’s cytotoxicity and genotoxicity is necessary. Thus, this study aimed to evaluate the possibility of IronQ inducing cytotoxicity and genotoxicity in peripheral blood mononuclear cells (PBMCs). We evaluated the vitality of cells, the production of reactive oxygen species (ROS), the level of antioxidant enzymes, and the stability of the genetic material in PBMCs treated with IronQ. The results show that IronQ had a negligible impact on toxicological parameters such as ROS production and lipid peroxidation, indicating that it is not harmful. IronQ-labeled PMBCs experienced an insignificant depletion of antioxidant enzyme levels at the highest concentration of IronQ. There is no evident genotoxicity in the magnetically IronQ-labeled PBMCs. The results show that IronQ does not potentiate the cytotoxicity and genotoxicity effects of the labeled PMBCs and might be safe for therapeutic and cell tracking purposes. These results could provide a reference guideline for the toxicological analysis of IronQ in in vivo studies.
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spelling pubmed-94145272022-08-27 Iron(III)–Quercetin Complexes’ Safety for MRI Cell Tracking in Cell Therapy Applications: Cytotoxic and Genotoxic Assessment Dechsupa, Nathupakorn Kosintarajit, Panida Kamkan, Kanyapak Khanjina, Thanyalak Sirikul, Chonticha Innuan, Phattarawadee Suwan, Authaphinya Anukul, Nampeung Kantapan, Jiraporn Nanomaterials (Basel) Article The theranostic agent iron–quercetin complex (IronQ) provides a T1-positive magnetic resonance imaging (MRI) contrast agent. The magnetically IronQ-labeled cells can be used for cell tracking and have active biological applications in promoting cell and tissue regeneration. However, a detailed investigation of IronQ’s cytotoxicity and genotoxicity is necessary. Thus, this study aimed to evaluate the possibility of IronQ inducing cytotoxicity and genotoxicity in peripheral blood mononuclear cells (PBMCs). We evaluated the vitality of cells, the production of reactive oxygen species (ROS), the level of antioxidant enzymes, and the stability of the genetic material in PBMCs treated with IronQ. The results show that IronQ had a negligible impact on toxicological parameters such as ROS production and lipid peroxidation, indicating that it is not harmful. IronQ-labeled PMBCs experienced an insignificant depletion of antioxidant enzyme levels at the highest concentration of IronQ. There is no evident genotoxicity in the magnetically IronQ-labeled PBMCs. The results show that IronQ does not potentiate the cytotoxicity and genotoxicity effects of the labeled PMBCs and might be safe for therapeutic and cell tracking purposes. These results could provide a reference guideline for the toxicological analysis of IronQ in in vivo studies. MDPI 2022-08-13 /pmc/articles/PMC9414527/ /pubmed/36014641 http://dx.doi.org/10.3390/nano12162776 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dechsupa, Nathupakorn
Kosintarajit, Panida
Kamkan, Kanyapak
Khanjina, Thanyalak
Sirikul, Chonticha
Innuan, Phattarawadee
Suwan, Authaphinya
Anukul, Nampeung
Kantapan, Jiraporn
Iron(III)–Quercetin Complexes’ Safety for MRI Cell Tracking in Cell Therapy Applications: Cytotoxic and Genotoxic Assessment
title Iron(III)–Quercetin Complexes’ Safety for MRI Cell Tracking in Cell Therapy Applications: Cytotoxic and Genotoxic Assessment
title_full Iron(III)–Quercetin Complexes’ Safety for MRI Cell Tracking in Cell Therapy Applications: Cytotoxic and Genotoxic Assessment
title_fullStr Iron(III)–Quercetin Complexes’ Safety for MRI Cell Tracking in Cell Therapy Applications: Cytotoxic and Genotoxic Assessment
title_full_unstemmed Iron(III)–Quercetin Complexes’ Safety for MRI Cell Tracking in Cell Therapy Applications: Cytotoxic and Genotoxic Assessment
title_short Iron(III)–Quercetin Complexes’ Safety for MRI Cell Tracking in Cell Therapy Applications: Cytotoxic and Genotoxic Assessment
title_sort iron(iii)–quercetin complexes’ safety for mri cell tracking in cell therapy applications: cytotoxic and genotoxic assessment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414527/
https://www.ncbi.nlm.nih.gov/pubmed/36014641
http://dx.doi.org/10.3390/nano12162776
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