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The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses

Porcine deltacoronavirus (PDCoV), belonging to family Coronaviridae and genus Deltacoronavirus, is a major enteric pathogen in swine. Accurate PDCoV diagnosis relying on laboratory testing and antibody detection is an important approach. This study evaluated the potential of the receptor-binding sub...

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Autores principales: Yen, Lu, Magtoto, Ronaldo, Mora-Díaz, Juan Carlos, Carrillo-Ávila, Jose Antonio, Zhang, Jianqiang, Cheng, Ting-Yu, Magtoto, Precy, Nelli, Rahul K., Baum, David H., Zimmerman, Jeffrey J., Giménez-Lirola, Luis G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414728/
https://www.ncbi.nlm.nih.gov/pubmed/36015031
http://dx.doi.org/10.3390/pathogens11080910
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author Yen, Lu
Magtoto, Ronaldo
Mora-Díaz, Juan Carlos
Carrillo-Ávila, Jose Antonio
Zhang, Jianqiang
Cheng, Ting-Yu
Magtoto, Precy
Nelli, Rahul K.
Baum, David H.
Zimmerman, Jeffrey J.
Giménez-Lirola, Luis G.
author_facet Yen, Lu
Magtoto, Ronaldo
Mora-Díaz, Juan Carlos
Carrillo-Ávila, Jose Antonio
Zhang, Jianqiang
Cheng, Ting-Yu
Magtoto, Precy
Nelli, Rahul K.
Baum, David H.
Zimmerman, Jeffrey J.
Giménez-Lirola, Luis G.
author_sort Yen, Lu
collection PubMed
description Porcine deltacoronavirus (PDCoV), belonging to family Coronaviridae and genus Deltacoronavirus, is a major enteric pathogen in swine. Accurate PDCoV diagnosis relying on laboratory testing and antibody detection is an important approach. This study evaluated the potential of the receptor-binding subunit of the PDCoV spike protein (S1), generated using a mammalian expression system, for specific antibody detection via indirect enzyme-linked immunosorbent assay (ELISA). Serum samples were collected at day post-inoculation (DPI) −7 to 42, from pigs (n = 83) experimentally inoculated with different porcine coronaviruses (PorCoV). The diagnostic sensitivity of the PDCoV S1-based ELISA was evaluated using serum samples (n = 72) from PDCoV-inoculated animals. The diagnostic specificity and potential cross-reactivity of the assay was evaluated on PorCoV-negative samples (n = 345) and samples collected from pigs experimentally inoculated with other PorCoVs (n = 472). The overall diagnostic performance, time of detection, and detection rate over time varied across different S/P cut-offs, estimated by Receiver Operating Characteristic (ROC) curve analysis. The higher detection rate in the PDCoV group was observed after DPI 21. An S/P cut-off of 0.25 provided 100% specificity with no serological cross-reactivity against other PorCoV. These results support the use of S1 protein-based ELISA for accurate detection of PDCoV infections, transference of maternal antibodies, or active surveillance.
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spelling pubmed-94147282022-08-27 The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses Yen, Lu Magtoto, Ronaldo Mora-Díaz, Juan Carlos Carrillo-Ávila, Jose Antonio Zhang, Jianqiang Cheng, Ting-Yu Magtoto, Precy Nelli, Rahul K. Baum, David H. Zimmerman, Jeffrey J. Giménez-Lirola, Luis G. Pathogens Article Porcine deltacoronavirus (PDCoV), belonging to family Coronaviridae and genus Deltacoronavirus, is a major enteric pathogen in swine. Accurate PDCoV diagnosis relying on laboratory testing and antibody detection is an important approach. This study evaluated the potential of the receptor-binding subunit of the PDCoV spike protein (S1), generated using a mammalian expression system, for specific antibody detection via indirect enzyme-linked immunosorbent assay (ELISA). Serum samples were collected at day post-inoculation (DPI) −7 to 42, from pigs (n = 83) experimentally inoculated with different porcine coronaviruses (PorCoV). The diagnostic sensitivity of the PDCoV S1-based ELISA was evaluated using serum samples (n = 72) from PDCoV-inoculated animals. The diagnostic specificity and potential cross-reactivity of the assay was evaluated on PorCoV-negative samples (n = 345) and samples collected from pigs experimentally inoculated with other PorCoVs (n = 472). The overall diagnostic performance, time of detection, and detection rate over time varied across different S/P cut-offs, estimated by Receiver Operating Characteristic (ROC) curve analysis. The higher detection rate in the PDCoV group was observed after DPI 21. An S/P cut-off of 0.25 provided 100% specificity with no serological cross-reactivity against other PorCoV. These results support the use of S1 protein-based ELISA for accurate detection of PDCoV infections, transference of maternal antibodies, or active surveillance. MDPI 2022-08-13 /pmc/articles/PMC9414728/ /pubmed/36015031 http://dx.doi.org/10.3390/pathogens11080910 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yen, Lu
Magtoto, Ronaldo
Mora-Díaz, Juan Carlos
Carrillo-Ávila, Jose Antonio
Zhang, Jianqiang
Cheng, Ting-Yu
Magtoto, Precy
Nelli, Rahul K.
Baum, David H.
Zimmerman, Jeffrey J.
Giménez-Lirola, Luis G.
The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses
title The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses
title_full The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses
title_fullStr The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses
title_full_unstemmed The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses
title_short The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses
title_sort n-terminal subunit of the porcine deltacoronavirus spike recombinant protein (s1) does not serologically cross-react with other porcine coronaviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414728/
https://www.ncbi.nlm.nih.gov/pubmed/36015031
http://dx.doi.org/10.3390/pathogens11080910
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