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The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses
Porcine deltacoronavirus (PDCoV), belonging to family Coronaviridae and genus Deltacoronavirus, is a major enteric pathogen in swine. Accurate PDCoV diagnosis relying on laboratory testing and antibody detection is an important approach. This study evaluated the potential of the receptor-binding sub...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414728/ https://www.ncbi.nlm.nih.gov/pubmed/36015031 http://dx.doi.org/10.3390/pathogens11080910 |
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author | Yen, Lu Magtoto, Ronaldo Mora-Díaz, Juan Carlos Carrillo-Ávila, Jose Antonio Zhang, Jianqiang Cheng, Ting-Yu Magtoto, Precy Nelli, Rahul K. Baum, David H. Zimmerman, Jeffrey J. Giménez-Lirola, Luis G. |
author_facet | Yen, Lu Magtoto, Ronaldo Mora-Díaz, Juan Carlos Carrillo-Ávila, Jose Antonio Zhang, Jianqiang Cheng, Ting-Yu Magtoto, Precy Nelli, Rahul K. Baum, David H. Zimmerman, Jeffrey J. Giménez-Lirola, Luis G. |
author_sort | Yen, Lu |
collection | PubMed |
description | Porcine deltacoronavirus (PDCoV), belonging to family Coronaviridae and genus Deltacoronavirus, is a major enteric pathogen in swine. Accurate PDCoV diagnosis relying on laboratory testing and antibody detection is an important approach. This study evaluated the potential of the receptor-binding subunit of the PDCoV spike protein (S1), generated using a mammalian expression system, for specific antibody detection via indirect enzyme-linked immunosorbent assay (ELISA). Serum samples were collected at day post-inoculation (DPI) −7 to 42, from pigs (n = 83) experimentally inoculated with different porcine coronaviruses (PorCoV). The diagnostic sensitivity of the PDCoV S1-based ELISA was evaluated using serum samples (n = 72) from PDCoV-inoculated animals. The diagnostic specificity and potential cross-reactivity of the assay was evaluated on PorCoV-negative samples (n = 345) and samples collected from pigs experimentally inoculated with other PorCoVs (n = 472). The overall diagnostic performance, time of detection, and detection rate over time varied across different S/P cut-offs, estimated by Receiver Operating Characteristic (ROC) curve analysis. The higher detection rate in the PDCoV group was observed after DPI 21. An S/P cut-off of 0.25 provided 100% specificity with no serological cross-reactivity against other PorCoV. These results support the use of S1 protein-based ELISA for accurate detection of PDCoV infections, transference of maternal antibodies, or active surveillance. |
format | Online Article Text |
id | pubmed-9414728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94147282022-08-27 The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses Yen, Lu Magtoto, Ronaldo Mora-Díaz, Juan Carlos Carrillo-Ávila, Jose Antonio Zhang, Jianqiang Cheng, Ting-Yu Magtoto, Precy Nelli, Rahul K. Baum, David H. Zimmerman, Jeffrey J. Giménez-Lirola, Luis G. Pathogens Article Porcine deltacoronavirus (PDCoV), belonging to family Coronaviridae and genus Deltacoronavirus, is a major enteric pathogen in swine. Accurate PDCoV diagnosis relying on laboratory testing and antibody detection is an important approach. This study evaluated the potential of the receptor-binding subunit of the PDCoV spike protein (S1), generated using a mammalian expression system, for specific antibody detection via indirect enzyme-linked immunosorbent assay (ELISA). Serum samples were collected at day post-inoculation (DPI) −7 to 42, from pigs (n = 83) experimentally inoculated with different porcine coronaviruses (PorCoV). The diagnostic sensitivity of the PDCoV S1-based ELISA was evaluated using serum samples (n = 72) from PDCoV-inoculated animals. The diagnostic specificity and potential cross-reactivity of the assay was evaluated on PorCoV-negative samples (n = 345) and samples collected from pigs experimentally inoculated with other PorCoVs (n = 472). The overall diagnostic performance, time of detection, and detection rate over time varied across different S/P cut-offs, estimated by Receiver Operating Characteristic (ROC) curve analysis. The higher detection rate in the PDCoV group was observed after DPI 21. An S/P cut-off of 0.25 provided 100% specificity with no serological cross-reactivity against other PorCoV. These results support the use of S1 protein-based ELISA for accurate detection of PDCoV infections, transference of maternal antibodies, or active surveillance. MDPI 2022-08-13 /pmc/articles/PMC9414728/ /pubmed/36015031 http://dx.doi.org/10.3390/pathogens11080910 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yen, Lu Magtoto, Ronaldo Mora-Díaz, Juan Carlos Carrillo-Ávila, Jose Antonio Zhang, Jianqiang Cheng, Ting-Yu Magtoto, Precy Nelli, Rahul K. Baum, David H. Zimmerman, Jeffrey J. Giménez-Lirola, Luis G. The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses |
title | The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses |
title_full | The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses |
title_fullStr | The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses |
title_full_unstemmed | The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses |
title_short | The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses |
title_sort | n-terminal subunit of the porcine deltacoronavirus spike recombinant protein (s1) does not serologically cross-react with other porcine coronaviruses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414728/ https://www.ncbi.nlm.nih.gov/pubmed/36015031 http://dx.doi.org/10.3390/pathogens11080910 |
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