Cargando…

Portulaca oleracea L. Extract Regulates Hepatic Cholesterol Metabolism via the AMPK/MicroRNA-33/34a Pathway in Rats Fed a High-Cholesterol Diet

This study examined the effect of extruded Portulaca oleracea L. extract (PE) in rats fed a high-cholesterol diet through the AMP-activated protein kinase (AMPK) and microRNA (miR)-33/34a pathway. Sprague–Dawley rats were randomized into three groups and fed either a standard diet (SD), a high-chole...

Descripción completa

Detalles Bibliográficos
Autores principales: Jang, Sojeong, Lee, Mak-Soon, Kang, Sun-A, Kim, Chong-Tai, Kim, Yangha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414803/
https://www.ncbi.nlm.nih.gov/pubmed/36014836
http://dx.doi.org/10.3390/nu14163330
_version_ 1784776077102546944
author Jang, Sojeong
Lee, Mak-Soon
Kang, Sun-A
Kim, Chong-Tai
Kim, Yangha
author_facet Jang, Sojeong
Lee, Mak-Soon
Kang, Sun-A
Kim, Chong-Tai
Kim, Yangha
author_sort Jang, Sojeong
collection PubMed
description This study examined the effect of extruded Portulaca oleracea L. extract (PE) in rats fed a high-cholesterol diet through the AMP-activated protein kinase (AMPK) and microRNA (miR)-33/34a pathway. Sprague–Dawley rats were randomized into three groups and fed either a standard diet (SD), a high-cholesterol diet containing 1% cholesterol and 0.5% cholic acid (HC), or an HC diet containing 0.8% PE for 4 weeks. PE supplementation improved serum, liver, and fecal lipid profiles. PE upregulated the expression of genes involved in cholesterol efflux and bile acids’ synthesis such as liver X receptor alpha (LXRα), ATP-binding cassette subfamily G5/G8 (ABCG5/8), and cholesterol 7 alpha-hydroxylase (CYP7A1), and downregulated farnesoid X receptor (FXR) in the liver. In addition, hepatic gene expression levels of apolipoprotein A-l (apoA-1), paraoxonase 1 (PON1), ATP-binding cassette subfamily A1/G1 (ABCA1/G1), lecithin-cholesterol acyltransferase (LCAT), and scavenger receptor class B type 1 (SR-B1), which are related to serum high-density lipoprotein cholesterol metabolism, were upregulated by PE. Furthermore, hepatic AMPK activity in the PE group was higher than in the HC group, and miR-33/34a expression levels were suppressed. These results suggest that PE improves the cholesterol metabolism by modulating AMPK activation and miR-33/34a expression in the liver.
format Online
Article
Text
id pubmed-9414803
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94148032022-08-27 Portulaca oleracea L. Extract Regulates Hepatic Cholesterol Metabolism via the AMPK/MicroRNA-33/34a Pathway in Rats Fed a High-Cholesterol Diet Jang, Sojeong Lee, Mak-Soon Kang, Sun-A Kim, Chong-Tai Kim, Yangha Nutrients Article This study examined the effect of extruded Portulaca oleracea L. extract (PE) in rats fed a high-cholesterol diet through the AMP-activated protein kinase (AMPK) and microRNA (miR)-33/34a pathway. Sprague–Dawley rats were randomized into three groups and fed either a standard diet (SD), a high-cholesterol diet containing 1% cholesterol and 0.5% cholic acid (HC), or an HC diet containing 0.8% PE for 4 weeks. PE supplementation improved serum, liver, and fecal lipid profiles. PE upregulated the expression of genes involved in cholesterol efflux and bile acids’ synthesis such as liver X receptor alpha (LXRα), ATP-binding cassette subfamily G5/G8 (ABCG5/8), and cholesterol 7 alpha-hydroxylase (CYP7A1), and downregulated farnesoid X receptor (FXR) in the liver. In addition, hepatic gene expression levels of apolipoprotein A-l (apoA-1), paraoxonase 1 (PON1), ATP-binding cassette subfamily A1/G1 (ABCA1/G1), lecithin-cholesterol acyltransferase (LCAT), and scavenger receptor class B type 1 (SR-B1), which are related to serum high-density lipoprotein cholesterol metabolism, were upregulated by PE. Furthermore, hepatic AMPK activity in the PE group was higher than in the HC group, and miR-33/34a expression levels were suppressed. These results suggest that PE improves the cholesterol metabolism by modulating AMPK activation and miR-33/34a expression in the liver. MDPI 2022-08-14 /pmc/articles/PMC9414803/ /pubmed/36014836 http://dx.doi.org/10.3390/nu14163330 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jang, Sojeong
Lee, Mak-Soon
Kang, Sun-A
Kim, Chong-Tai
Kim, Yangha
Portulaca oleracea L. Extract Regulates Hepatic Cholesterol Metabolism via the AMPK/MicroRNA-33/34a Pathway in Rats Fed a High-Cholesterol Diet
title Portulaca oleracea L. Extract Regulates Hepatic Cholesterol Metabolism via the AMPK/MicroRNA-33/34a Pathway in Rats Fed a High-Cholesterol Diet
title_full Portulaca oleracea L. Extract Regulates Hepatic Cholesterol Metabolism via the AMPK/MicroRNA-33/34a Pathway in Rats Fed a High-Cholesterol Diet
title_fullStr Portulaca oleracea L. Extract Regulates Hepatic Cholesterol Metabolism via the AMPK/MicroRNA-33/34a Pathway in Rats Fed a High-Cholesterol Diet
title_full_unstemmed Portulaca oleracea L. Extract Regulates Hepatic Cholesterol Metabolism via the AMPK/MicroRNA-33/34a Pathway in Rats Fed a High-Cholesterol Diet
title_short Portulaca oleracea L. Extract Regulates Hepatic Cholesterol Metabolism via the AMPK/MicroRNA-33/34a Pathway in Rats Fed a High-Cholesterol Diet
title_sort portulaca oleracea l. extract regulates hepatic cholesterol metabolism via the ampk/microrna-33/34a pathway in rats fed a high-cholesterol diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414803/
https://www.ncbi.nlm.nih.gov/pubmed/36014836
http://dx.doi.org/10.3390/nu14163330
work_keys_str_mv AT jangsojeong portulacaoleracealextractregulateshepaticcholesterolmetabolismviatheampkmicrorna3334apathwayinratsfedahighcholesteroldiet
AT leemaksoon portulacaoleracealextractregulateshepaticcholesterolmetabolismviatheampkmicrorna3334apathwayinratsfedahighcholesteroldiet
AT kangsuna portulacaoleracealextractregulateshepaticcholesterolmetabolismviatheampkmicrorna3334apathwayinratsfedahighcholesteroldiet
AT kimchongtai portulacaoleracealextractregulateshepaticcholesterolmetabolismviatheampkmicrorna3334apathwayinratsfedahighcholesteroldiet
AT kimyangha portulacaoleracealextractregulateshepaticcholesterolmetabolismviatheampkmicrorna3334apathwayinratsfedahighcholesteroldiet