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In Vitro Interactions of Amphiphilic Phosphorous Dendrons with Liposomes and Exosomes—Implications for Blood Viscosity Changes
Drug delivery by dendron-based nanoparticles is widely studied due to their ability to encapsulate or bind different ligands. For medical purposes, it is necessary (even if not sufficient) for these nanostructures to be compatible with blood. We studied the interaction of amphiphilic dendrons with b...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414926/ https://www.ncbi.nlm.nih.gov/pubmed/36015222 http://dx.doi.org/10.3390/pharmaceutics14081596 |
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author | Veliskova, Martina Zvarik, Milan Suty, Simon Jacko, Juraj Mydla, Patrick Cechova, Katarina Dzubinska, Daniela Morvova, Marcela Ionov, Maksim Terehova, Maria Majoral, Jean-Pierre Bryszewska, Maria Waczulikova, Iveta |
author_facet | Veliskova, Martina Zvarik, Milan Suty, Simon Jacko, Juraj Mydla, Patrick Cechova, Katarina Dzubinska, Daniela Morvova, Marcela Ionov, Maksim Terehova, Maria Majoral, Jean-Pierre Bryszewska, Maria Waczulikova, Iveta |
author_sort | Veliskova, Martina |
collection | PubMed |
description | Drug delivery by dendron-based nanoparticles is widely studied due to their ability to encapsulate or bind different ligands. For medical purposes, it is necessary (even if not sufficient) for these nanostructures to be compatible with blood. We studied the interaction of amphiphilic dendrons with blood samples from healthy volunteers using standard laboratory methods and rheological measurements. We did not observe clinically relevant abnormalities, but we found a concentration-dependent increase in whole blood viscosity, higher in males, presumably due to the formation of aggregates. To characterize the nature of the interactions among blood components and dendrons, we performed experiments on the liposomes and exosomes as models of biological membranes. Based on results obtained using diverse biophysical methods, we conclude that the interactions were of electrostatic nature. Overall, we have confirmed a concentration-dependent effect of dendrons on membrane systems, while the effect of generation was ambiguous. At higher dendron concentrations, the structure of membranes became disturbed, and membranes were prone to forming bigger aggregates, as visualized by SEM. This might have implications for blood flow disturbances when used in vivo. We propose to introduce blood viscosity measurements in early stages of investigation as they can help to optimize drug-like properties of potential drug carriers. |
format | Online Article Text |
id | pubmed-9414926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94149262022-08-27 In Vitro Interactions of Amphiphilic Phosphorous Dendrons with Liposomes and Exosomes—Implications for Blood Viscosity Changes Veliskova, Martina Zvarik, Milan Suty, Simon Jacko, Juraj Mydla, Patrick Cechova, Katarina Dzubinska, Daniela Morvova, Marcela Ionov, Maksim Terehova, Maria Majoral, Jean-Pierre Bryszewska, Maria Waczulikova, Iveta Pharmaceutics Article Drug delivery by dendron-based nanoparticles is widely studied due to their ability to encapsulate or bind different ligands. For medical purposes, it is necessary (even if not sufficient) for these nanostructures to be compatible with blood. We studied the interaction of amphiphilic dendrons with blood samples from healthy volunteers using standard laboratory methods and rheological measurements. We did not observe clinically relevant abnormalities, but we found a concentration-dependent increase in whole blood viscosity, higher in males, presumably due to the formation of aggregates. To characterize the nature of the interactions among blood components and dendrons, we performed experiments on the liposomes and exosomes as models of biological membranes. Based on results obtained using diverse biophysical methods, we conclude that the interactions were of electrostatic nature. Overall, we have confirmed a concentration-dependent effect of dendrons on membrane systems, while the effect of generation was ambiguous. At higher dendron concentrations, the structure of membranes became disturbed, and membranes were prone to forming bigger aggregates, as visualized by SEM. This might have implications for blood flow disturbances when used in vivo. We propose to introduce blood viscosity measurements in early stages of investigation as they can help to optimize drug-like properties of potential drug carriers. MDPI 2022-07-30 /pmc/articles/PMC9414926/ /pubmed/36015222 http://dx.doi.org/10.3390/pharmaceutics14081596 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Veliskova, Martina Zvarik, Milan Suty, Simon Jacko, Juraj Mydla, Patrick Cechova, Katarina Dzubinska, Daniela Morvova, Marcela Ionov, Maksim Terehova, Maria Majoral, Jean-Pierre Bryszewska, Maria Waczulikova, Iveta In Vitro Interactions of Amphiphilic Phosphorous Dendrons with Liposomes and Exosomes—Implications for Blood Viscosity Changes |
title | In Vitro Interactions of Amphiphilic Phosphorous Dendrons with Liposomes and Exosomes—Implications for Blood Viscosity Changes |
title_full | In Vitro Interactions of Amphiphilic Phosphorous Dendrons with Liposomes and Exosomes—Implications for Blood Viscosity Changes |
title_fullStr | In Vitro Interactions of Amphiphilic Phosphorous Dendrons with Liposomes and Exosomes—Implications for Blood Viscosity Changes |
title_full_unstemmed | In Vitro Interactions of Amphiphilic Phosphorous Dendrons with Liposomes and Exosomes—Implications for Blood Viscosity Changes |
title_short | In Vitro Interactions of Amphiphilic Phosphorous Dendrons with Liposomes and Exosomes—Implications for Blood Viscosity Changes |
title_sort | in vitro interactions of amphiphilic phosphorous dendrons with liposomes and exosomes—implications for blood viscosity changes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414926/ https://www.ncbi.nlm.nih.gov/pubmed/36015222 http://dx.doi.org/10.3390/pharmaceutics14081596 |
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