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Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics

Metabolic fatty liver disease caused by high-starch diet restricted the intensive and sustainable development of carnivorous fish such as largemouth bass. In this study, the combination liver proteomic and lipidomic approach was employed to investigate the key signaling pathways and identify the cri...

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Autores principales: Xue, Moyong, Yao, Ting, Xue, Min, Francis, Frédéric, Qin, Yuchang, Jia, Ming, Li, Junguo, Gu, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415018/
https://www.ncbi.nlm.nih.gov/pubmed/36005631
http://dx.doi.org/10.3390/metabo12080759
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author Xue, Moyong
Yao, Ting
Xue, Min
Francis, Frédéric
Qin, Yuchang
Jia, Ming
Li, Junguo
Gu, Xu
author_facet Xue, Moyong
Yao, Ting
Xue, Min
Francis, Frédéric
Qin, Yuchang
Jia, Ming
Li, Junguo
Gu, Xu
author_sort Xue, Moyong
collection PubMed
description Metabolic fatty liver disease caused by high-starch diet restricted the intensive and sustainable development of carnivorous fish such as largemouth bass. In this study, the combination liver proteomic and lipidomic approach was employed to investigate the key signaling pathways and identify the critical biomarkers of fatty liver in largemouth bass. Joint analysis of the correlated differential proteins and lipids revealed nine common metabolic pathways; it was determined that FABP1 were significantly up-regulated in terms of transporting more triglycerides into the liver, while ABCA1 and VDAC1 proteins were significantly down-regulated in terms of preventing the transport of lipids and cholesterol out of the liver, leading to triglyceride accumulation in hepatocyte, eventually resulting in metabolic fatty liver disease. The results indicate that FABP1, ABCA1 and VDAC1 could be potential biomarkers for treating metabolic fatty liver disease of largemouth bass.
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spelling pubmed-94150182022-08-27 Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics Xue, Moyong Yao, Ting Xue, Min Francis, Frédéric Qin, Yuchang Jia, Ming Li, Junguo Gu, Xu Metabolites Article Metabolic fatty liver disease caused by high-starch diet restricted the intensive and sustainable development of carnivorous fish such as largemouth bass. In this study, the combination liver proteomic and lipidomic approach was employed to investigate the key signaling pathways and identify the critical biomarkers of fatty liver in largemouth bass. Joint analysis of the correlated differential proteins and lipids revealed nine common metabolic pathways; it was determined that FABP1 were significantly up-regulated in terms of transporting more triglycerides into the liver, while ABCA1 and VDAC1 proteins were significantly down-regulated in terms of preventing the transport of lipids and cholesterol out of the liver, leading to triglyceride accumulation in hepatocyte, eventually resulting in metabolic fatty liver disease. The results indicate that FABP1, ABCA1 and VDAC1 could be potential biomarkers for treating metabolic fatty liver disease of largemouth bass. MDPI 2022-08-17 /pmc/articles/PMC9415018/ /pubmed/36005631 http://dx.doi.org/10.3390/metabo12080759 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xue, Moyong
Yao, Ting
Xue, Min
Francis, Frédéric
Qin, Yuchang
Jia, Ming
Li, Junguo
Gu, Xu
Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics
title Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics
title_full Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics
title_fullStr Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics
title_full_unstemmed Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics
title_short Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics
title_sort mechanism analysis of metabolic fatty liver on largemouth bass (micropterus salmoides) based on integrated lipidomics and proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415018/
https://www.ncbi.nlm.nih.gov/pubmed/36005631
http://dx.doi.org/10.3390/metabo12080759
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