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Mexican Strains of Anaplasma marginale: A First Comparative Genomics and Phylogeographic Analysis

The One Health approach looks after animal welfare and demands constant monitoring of the strains that circulate globally to prevent outbreaks. Anaplasma marginale is the etiologic agent of bovine anaplasmosis and is endemic worldwide. This study aimed to analyze, for the first time, the genetic div...

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Autores principales: Dantán-González, Edgar, Quiroz-Castañeda, Rosa Estela, Aguilar-Díaz, Hugo, Amaro-Estrada, Itzel, Martínez-Ocampo, Fernando, Rodríguez-Camarillo, Sergio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415054/
https://www.ncbi.nlm.nih.gov/pubmed/36014994
http://dx.doi.org/10.3390/pathogens11080873
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author Dantán-González, Edgar
Quiroz-Castañeda, Rosa Estela
Aguilar-Díaz, Hugo
Amaro-Estrada, Itzel
Martínez-Ocampo, Fernando
Rodríguez-Camarillo, Sergio
author_facet Dantán-González, Edgar
Quiroz-Castañeda, Rosa Estela
Aguilar-Díaz, Hugo
Amaro-Estrada, Itzel
Martínez-Ocampo, Fernando
Rodríguez-Camarillo, Sergio
author_sort Dantán-González, Edgar
collection PubMed
description The One Health approach looks after animal welfare and demands constant monitoring of the strains that circulate globally to prevent outbreaks. Anaplasma marginale is the etiologic agent of bovine anaplasmosis and is endemic worldwide. This study aimed to analyze, for the first time, the genetic diversity of seven Mexican strains of A. marginale and their relationship with other strains reported. The main features of A. marginale were obtained by characterizing all 24 genomes reported so far. Genetic diversity and phylogeography were analyzed by characterizing the msp1a gene and 5′-UTR microsatellite sequences and constructing a phylogenetic tree with 540 concatenated genes of the core genome. The Mexican strains show 15 different repeat sequences in six MSP1a structures and have phylogeographic relationships with strains from North America, South America, and Asia, which confirms they are highly variable. Based on our results, we encourage the performance of genome sequencing of A. marginale strains to obtain a high assembly level of molecular markers and the performance of extensive phylogeographic analysis. Undoubtedly, genomic surveillance helps build a picture of how a pathogen changes and evolves in geographical regions. However, we cannot discard the study of relationships pathogens establish with ticks and how they have co-evolved to establish themselves as a successful transmission system.
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spelling pubmed-94150542022-08-27 Mexican Strains of Anaplasma marginale: A First Comparative Genomics and Phylogeographic Analysis Dantán-González, Edgar Quiroz-Castañeda, Rosa Estela Aguilar-Díaz, Hugo Amaro-Estrada, Itzel Martínez-Ocampo, Fernando Rodríguez-Camarillo, Sergio Pathogens Article The One Health approach looks after animal welfare and demands constant monitoring of the strains that circulate globally to prevent outbreaks. Anaplasma marginale is the etiologic agent of bovine anaplasmosis and is endemic worldwide. This study aimed to analyze, for the first time, the genetic diversity of seven Mexican strains of A. marginale and their relationship with other strains reported. The main features of A. marginale were obtained by characterizing all 24 genomes reported so far. Genetic diversity and phylogeography were analyzed by characterizing the msp1a gene and 5′-UTR microsatellite sequences and constructing a phylogenetic tree with 540 concatenated genes of the core genome. The Mexican strains show 15 different repeat sequences in six MSP1a structures and have phylogeographic relationships with strains from North America, South America, and Asia, which confirms they are highly variable. Based on our results, we encourage the performance of genome sequencing of A. marginale strains to obtain a high assembly level of molecular markers and the performance of extensive phylogeographic analysis. Undoubtedly, genomic surveillance helps build a picture of how a pathogen changes and evolves in geographical regions. However, we cannot discard the study of relationships pathogens establish with ticks and how they have co-evolved to establish themselves as a successful transmission system. MDPI 2022-08-02 /pmc/articles/PMC9415054/ /pubmed/36014994 http://dx.doi.org/10.3390/pathogens11080873 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dantán-González, Edgar
Quiroz-Castañeda, Rosa Estela
Aguilar-Díaz, Hugo
Amaro-Estrada, Itzel
Martínez-Ocampo, Fernando
Rodríguez-Camarillo, Sergio
Mexican Strains of Anaplasma marginale: A First Comparative Genomics and Phylogeographic Analysis
title Mexican Strains of Anaplasma marginale: A First Comparative Genomics and Phylogeographic Analysis
title_full Mexican Strains of Anaplasma marginale: A First Comparative Genomics and Phylogeographic Analysis
title_fullStr Mexican Strains of Anaplasma marginale: A First Comparative Genomics and Phylogeographic Analysis
title_full_unstemmed Mexican Strains of Anaplasma marginale: A First Comparative Genomics and Phylogeographic Analysis
title_short Mexican Strains of Anaplasma marginale: A First Comparative Genomics and Phylogeographic Analysis
title_sort mexican strains of anaplasma marginale: a first comparative genomics and phylogeographic analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415054/
https://www.ncbi.nlm.nih.gov/pubmed/36014994
http://dx.doi.org/10.3390/pathogens11080873
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