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Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic

New antibiotics are seen as ‘drugs of last resort’ against virulent bacteria. However, development of resistance towards new antibiotics with time is a universal fact. Delafloxacin (DFX) is a new fluoroquinolone antibiotic that differs from existing fluoroquinolones by the lack of a protonatable sub...

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Autores principales: Abu Lila, Amr Selim, Huwaimel, Bader, Alobaida, Ahmed, Hussain, Talib, Rafi, Zeeshan, Mehmood, Khalid, Abdallah, Marwa H., Hagbani, Turki Al, Rizvi, Syed Mohd Danish, Moin, Afrasim, Ahmed, Abobakr F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415438/
https://www.ncbi.nlm.nih.gov/pubmed/36013845
http://dx.doi.org/10.3390/ma15165709
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author Abu Lila, Amr Selim
Huwaimel, Bader
Alobaida, Ahmed
Hussain, Talib
Rafi, Zeeshan
Mehmood, Khalid
Abdallah, Marwa H.
Hagbani, Turki Al
Rizvi, Syed Mohd Danish
Moin, Afrasim
Ahmed, Abobakr F.
author_facet Abu Lila, Amr Selim
Huwaimel, Bader
Alobaida, Ahmed
Hussain, Talib
Rafi, Zeeshan
Mehmood, Khalid
Abdallah, Marwa H.
Hagbani, Turki Al
Rizvi, Syed Mohd Danish
Moin, Afrasim
Ahmed, Abobakr F.
author_sort Abu Lila, Amr Selim
collection PubMed
description New antibiotics are seen as ‘drugs of last resort’ against virulent bacteria. However, development of resistance towards new antibiotics with time is a universal fact. Delafloxacin (DFX) is a new fluoroquinolone antibiotic that differs from existing fluoroquinolones by the lack of a protonatable substituent, which gives the molecule a weakly acidic nature, affording it higher antibacterial activity under an acidic environment. Furthermore, antibiotic-functionalized metallic nanoparticles have been recently emerged as a feasible platform for conquering bacterial resistance. In the present study, therefore, we aimed at preparing DFX-gold nano-formulations to increase the antibacterial potential of DFX. To synthesize DFX-capped gold nanoparticles (DFX-AuNPs), DFX was used as a reducing and stabilizing/encapsulating agent. Various analytical techniques such as UV-visible spectroscopy, TEM, DLS, FTIR and zeta potential analysis were applied to determine the properties of the synthesized DFX-AuNPs. The synthesized DFX-AuNPs revealed a distinct surface plasmon resonance (SPR) band at 530 nm and an average size of 16 nm as manifested by TEM analysis. In addition, Zeta potential results (−19 mV) confirmed the stability of the synthesized DFX-AuNPs. Furthermore, FTIR analysis demonstrated that DFX was adsorbed onto the surface of AuNPs via strong interaction between AuNPs and DFX. Most importantly, comparative antibacterial analysis of DFX alone and DFX-AuNPs against Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) verified the superior antibacterial activity of DFX-AuNPs against the tested microorganisms. To sum up, DFX gold nano-formulations can offer a promising possible solution, even at a lower antibiotic dose, to combat pathogenic bacteria.
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spelling pubmed-94154382022-08-27 Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic Abu Lila, Amr Selim Huwaimel, Bader Alobaida, Ahmed Hussain, Talib Rafi, Zeeshan Mehmood, Khalid Abdallah, Marwa H. Hagbani, Turki Al Rizvi, Syed Mohd Danish Moin, Afrasim Ahmed, Abobakr F. Materials (Basel) Article New antibiotics are seen as ‘drugs of last resort’ against virulent bacteria. However, development of resistance towards new antibiotics with time is a universal fact. Delafloxacin (DFX) is a new fluoroquinolone antibiotic that differs from existing fluoroquinolones by the lack of a protonatable substituent, which gives the molecule a weakly acidic nature, affording it higher antibacterial activity under an acidic environment. Furthermore, antibiotic-functionalized metallic nanoparticles have been recently emerged as a feasible platform for conquering bacterial resistance. In the present study, therefore, we aimed at preparing DFX-gold nano-formulations to increase the antibacterial potential of DFX. To synthesize DFX-capped gold nanoparticles (DFX-AuNPs), DFX was used as a reducing and stabilizing/encapsulating agent. Various analytical techniques such as UV-visible spectroscopy, TEM, DLS, FTIR and zeta potential analysis were applied to determine the properties of the synthesized DFX-AuNPs. The synthesized DFX-AuNPs revealed a distinct surface plasmon resonance (SPR) band at 530 nm and an average size of 16 nm as manifested by TEM analysis. In addition, Zeta potential results (−19 mV) confirmed the stability of the synthesized DFX-AuNPs. Furthermore, FTIR analysis demonstrated that DFX was adsorbed onto the surface of AuNPs via strong interaction between AuNPs and DFX. Most importantly, comparative antibacterial analysis of DFX alone and DFX-AuNPs against Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) verified the superior antibacterial activity of DFX-AuNPs against the tested microorganisms. To sum up, DFX gold nano-formulations can offer a promising possible solution, even at a lower antibiotic dose, to combat pathogenic bacteria. MDPI 2022-08-18 /pmc/articles/PMC9415438/ /pubmed/36013845 http://dx.doi.org/10.3390/ma15165709 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abu Lila, Amr Selim
Huwaimel, Bader
Alobaida, Ahmed
Hussain, Talib
Rafi, Zeeshan
Mehmood, Khalid
Abdallah, Marwa H.
Hagbani, Turki Al
Rizvi, Syed Mohd Danish
Moin, Afrasim
Ahmed, Abobakr F.
Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic
title Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic
title_full Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic
title_fullStr Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic
title_full_unstemmed Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic
title_short Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic
title_sort delafloxacin-capped gold nanoparticles (dfx-aunps): an effective antibacterial nano-formulation of fluoroquinolone antibiotic
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415438/
https://www.ncbi.nlm.nih.gov/pubmed/36013845
http://dx.doi.org/10.3390/ma15165709
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