Cargando…
Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications
Research relating to dielectrophoresis (DEP) has been progressing rapidly through time as it is a strong and controllable technique for manipulation, separation, preconcentration, and partitioning of protein. Extensive studies have been carried out on protein DEP, especially on Bovine Serum Albumin...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415755/ https://www.ncbi.nlm.nih.gov/pubmed/36014230 http://dx.doi.org/10.3390/mi13081308 |
_version_ | 1784776310041608192 |
---|---|
author | Nasir, Nur Shahira Abdul Deivasigamani, Revathy Wee, M. F. Mohd Razip Hamzah, Azrul Azlan Zaid, Mohd Hazani Mat Rahim, Muhammad Khairulanwar Abdul Kayani, Aminuddin Ahmad Abdulhameed, Abdullah Buyong, Muhamad Ramdzan |
author_facet | Nasir, Nur Shahira Abdul Deivasigamani, Revathy Wee, M. F. Mohd Razip Hamzah, Azrul Azlan Zaid, Mohd Hazani Mat Rahim, Muhammad Khairulanwar Abdul Kayani, Aminuddin Ahmad Abdulhameed, Abdullah Buyong, Muhamad Ramdzan |
author_sort | Nasir, Nur Shahira Abdul |
collection | PubMed |
description | Research relating to dielectrophoresis (DEP) has been progressing rapidly through time as it is a strong and controllable technique for manipulation, separation, preconcentration, and partitioning of protein. Extensive studies have been carried out on protein DEP, especially on Bovine Serum Albumin (BSA). However, these studies involve the usage of dye and fluorescent probes to observe DEP responses as the physical properties of protein albumin molecular structure are translucent. The use of dye and the fluorescent probe could later affect the protein’s physiology. In this article, we review three methods of electrical quantification of DEP responses: electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and capacitance measurement for protein BSA DEP manipulation. The correlation of these methods with DEP responses is further discussed. Based on the observations on capacitance measurement, it can be deduced that the electrical quantifying method is reliable for identifying DEP responses. Further, the possibility of manipulating the protein and electrically quantifying DEP responses while retaining the original physiology of the protein and without the usage of dye or fluorescent probe is discussed. |
format | Online Article Text |
id | pubmed-9415755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94157552022-08-27 Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications Nasir, Nur Shahira Abdul Deivasigamani, Revathy Wee, M. F. Mohd Razip Hamzah, Azrul Azlan Zaid, Mohd Hazani Mat Rahim, Muhammad Khairulanwar Abdul Kayani, Aminuddin Ahmad Abdulhameed, Abdullah Buyong, Muhamad Ramdzan Micromachines (Basel) Review Research relating to dielectrophoresis (DEP) has been progressing rapidly through time as it is a strong and controllable technique for manipulation, separation, preconcentration, and partitioning of protein. Extensive studies have been carried out on protein DEP, especially on Bovine Serum Albumin (BSA). However, these studies involve the usage of dye and fluorescent probes to observe DEP responses as the physical properties of protein albumin molecular structure are translucent. The use of dye and the fluorescent probe could later affect the protein’s physiology. In this article, we review three methods of electrical quantification of DEP responses: electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and capacitance measurement for protein BSA DEP manipulation. The correlation of these methods with DEP responses is further discussed. Based on the observations on capacitance measurement, it can be deduced that the electrical quantifying method is reliable for identifying DEP responses. Further, the possibility of manipulating the protein and electrically quantifying DEP responses while retaining the original physiology of the protein and without the usage of dye or fluorescent probe is discussed. MDPI 2022-08-13 /pmc/articles/PMC9415755/ /pubmed/36014230 http://dx.doi.org/10.3390/mi13081308 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Nasir, Nur Shahira Abdul Deivasigamani, Revathy Wee, M. F. Mohd Razip Hamzah, Azrul Azlan Zaid, Mohd Hazani Mat Rahim, Muhammad Khairulanwar Abdul Kayani, Aminuddin Ahmad Abdulhameed, Abdullah Buyong, Muhamad Ramdzan Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications |
title | Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications |
title_full | Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications |
title_fullStr | Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications |
title_full_unstemmed | Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications |
title_short | Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications |
title_sort | protein albumin manipulation and electrical quantification of molecular dielectrophoresis responses for biomedical applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415755/ https://www.ncbi.nlm.nih.gov/pubmed/36014230 http://dx.doi.org/10.3390/mi13081308 |
work_keys_str_mv | AT nasirnurshahiraabdul proteinalbuminmanipulationandelectricalquantificationofmoleculardielectrophoresisresponsesforbiomedicalapplications AT deivasigamanirevathy proteinalbuminmanipulationandelectricalquantificationofmoleculardielectrophoresisresponsesforbiomedicalapplications AT weemfmohdrazip proteinalbuminmanipulationandelectricalquantificationofmoleculardielectrophoresisresponsesforbiomedicalapplications AT hamzahazrulazlan proteinalbuminmanipulationandelectricalquantificationofmoleculardielectrophoresisresponsesforbiomedicalapplications AT zaidmohdhazanimat proteinalbuminmanipulationandelectricalquantificationofmoleculardielectrophoresisresponsesforbiomedicalapplications AT rahimmuhammadkhairulanwarabdul proteinalbuminmanipulationandelectricalquantificationofmoleculardielectrophoresisresponsesforbiomedicalapplications AT kayaniaminuddinahmad proteinalbuminmanipulationandelectricalquantificationofmoleculardielectrophoresisresponsesforbiomedicalapplications AT abdulhameedabdullah proteinalbuminmanipulationandelectricalquantificationofmoleculardielectrophoresisresponsesforbiomedicalapplications AT buyongmuhamadramdzan proteinalbuminmanipulationandelectricalquantificationofmoleculardielectrophoresisresponsesforbiomedicalapplications |