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Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications

Research relating to dielectrophoresis (DEP) has been progressing rapidly through time as it is a strong and controllable technique for manipulation, separation, preconcentration, and partitioning of protein. Extensive studies have been carried out on protein DEP, especially on Bovine Serum Albumin...

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Autores principales: Nasir, Nur Shahira Abdul, Deivasigamani, Revathy, Wee, M. F. Mohd Razip, Hamzah, Azrul Azlan, Zaid, Mohd Hazani Mat, Rahim, Muhammad Khairulanwar Abdul, Kayani, Aminuddin Ahmad, Abdulhameed, Abdullah, Buyong, Muhamad Ramdzan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415755/
https://www.ncbi.nlm.nih.gov/pubmed/36014230
http://dx.doi.org/10.3390/mi13081308
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author Nasir, Nur Shahira Abdul
Deivasigamani, Revathy
Wee, M. F. Mohd Razip
Hamzah, Azrul Azlan
Zaid, Mohd Hazani Mat
Rahim, Muhammad Khairulanwar Abdul
Kayani, Aminuddin Ahmad
Abdulhameed, Abdullah
Buyong, Muhamad Ramdzan
author_facet Nasir, Nur Shahira Abdul
Deivasigamani, Revathy
Wee, M. F. Mohd Razip
Hamzah, Azrul Azlan
Zaid, Mohd Hazani Mat
Rahim, Muhammad Khairulanwar Abdul
Kayani, Aminuddin Ahmad
Abdulhameed, Abdullah
Buyong, Muhamad Ramdzan
author_sort Nasir, Nur Shahira Abdul
collection PubMed
description Research relating to dielectrophoresis (DEP) has been progressing rapidly through time as it is a strong and controllable technique for manipulation, separation, preconcentration, and partitioning of protein. Extensive studies have been carried out on protein DEP, especially on Bovine Serum Albumin (BSA). However, these studies involve the usage of dye and fluorescent probes to observe DEP responses as the physical properties of protein albumin molecular structure are translucent. The use of dye and the fluorescent probe could later affect the protein’s physiology. In this article, we review three methods of electrical quantification of DEP responses: electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and capacitance measurement for protein BSA DEP manipulation. The correlation of these methods with DEP responses is further discussed. Based on the observations on capacitance measurement, it can be deduced that the electrical quantifying method is reliable for identifying DEP responses. Further, the possibility of manipulating the protein and electrically quantifying DEP responses while retaining the original physiology of the protein and without the usage of dye or fluorescent probe is discussed.
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spelling pubmed-94157552022-08-27 Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications Nasir, Nur Shahira Abdul Deivasigamani, Revathy Wee, M. F. Mohd Razip Hamzah, Azrul Azlan Zaid, Mohd Hazani Mat Rahim, Muhammad Khairulanwar Abdul Kayani, Aminuddin Ahmad Abdulhameed, Abdullah Buyong, Muhamad Ramdzan Micromachines (Basel) Review Research relating to dielectrophoresis (DEP) has been progressing rapidly through time as it is a strong and controllable technique for manipulation, separation, preconcentration, and partitioning of protein. Extensive studies have been carried out on protein DEP, especially on Bovine Serum Albumin (BSA). However, these studies involve the usage of dye and fluorescent probes to observe DEP responses as the physical properties of protein albumin molecular structure are translucent. The use of dye and the fluorescent probe could later affect the protein’s physiology. In this article, we review three methods of electrical quantification of DEP responses: electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and capacitance measurement for protein BSA DEP manipulation. The correlation of these methods with DEP responses is further discussed. Based on the observations on capacitance measurement, it can be deduced that the electrical quantifying method is reliable for identifying DEP responses. Further, the possibility of manipulating the protein and electrically quantifying DEP responses while retaining the original physiology of the protein and without the usage of dye or fluorescent probe is discussed. MDPI 2022-08-13 /pmc/articles/PMC9415755/ /pubmed/36014230 http://dx.doi.org/10.3390/mi13081308 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Nasir, Nur Shahira Abdul
Deivasigamani, Revathy
Wee, M. F. Mohd Razip
Hamzah, Azrul Azlan
Zaid, Mohd Hazani Mat
Rahim, Muhammad Khairulanwar Abdul
Kayani, Aminuddin Ahmad
Abdulhameed, Abdullah
Buyong, Muhamad Ramdzan
Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications
title Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications
title_full Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications
title_fullStr Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications
title_full_unstemmed Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications
title_short Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications
title_sort protein albumin manipulation and electrical quantification of molecular dielectrophoresis responses for biomedical applications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415755/
https://www.ncbi.nlm.nih.gov/pubmed/36014230
http://dx.doi.org/10.3390/mi13081308
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