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Characterization of Host-Specific Genes from Pine- and Grass-Associated Species of the Fusarium fujikuroi Species Complex

The Fusarium fujikuroi species complex (FFSC) includes socioeconomically important pathogens that cause disease for numerous crops and synthesize a variety of secondary metabolites that can contaminate feedstocks and food. Here, we used comparative genomics to elucidate processes underlying the abil...

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Autores principales: Dewing, Claudette, Van der Nest, Magrieta A., Santana, Quentin C., Proctor, Robert H., Wingfield, Brenda D., Steenkamp, Emma T., De Vos, Lieschen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415769/
https://www.ncbi.nlm.nih.gov/pubmed/36014979
http://dx.doi.org/10.3390/pathogens11080858
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author Dewing, Claudette
Van der Nest, Magrieta A.
Santana, Quentin C.
Proctor, Robert H.
Wingfield, Brenda D.
Steenkamp, Emma T.
De Vos, Lieschen
author_facet Dewing, Claudette
Van der Nest, Magrieta A.
Santana, Quentin C.
Proctor, Robert H.
Wingfield, Brenda D.
Steenkamp, Emma T.
De Vos, Lieschen
author_sort Dewing, Claudette
collection PubMed
description The Fusarium fujikuroi species complex (FFSC) includes socioeconomically important pathogens that cause disease for numerous crops and synthesize a variety of secondary metabolites that can contaminate feedstocks and food. Here, we used comparative genomics to elucidate processes underlying the ability of pine-associated and grass-associated FFSC species to colonize tissues of their respective plant hosts. We characterized the identity, possible functions, evolutionary origins, and chromosomal positions of the host-range-associated genes encoded by the two groups of fungi. The 72 and 47 genes identified as unique to the respective genome groups were potentially involved in diverse processes, ranging from transcription, regulation, and substrate transport through to virulence/pathogenicity. Most genes arose early during the evolution of Fusarium/FFSC and were only subsequently retained in some lineages, while some had origins outside Fusarium. Although differences in the densities of these genes were especially noticeable on the conditionally dispensable chromosome of F. temperatum (representing the grass-associates) and F. circinatum (representing the pine-associates), the host-range-associated genes tended to be located towards the subtelomeric regions of chromosomes. Taken together, these results demonstrate that multiple mechanisms drive the emergence of genes in the grass- and pine-associated FFSC taxa examined. It also highlighted the diversity of the molecular processes potentially underlying niche-specificity in these and other Fusarium species.
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spelling pubmed-94157692022-08-27 Characterization of Host-Specific Genes from Pine- and Grass-Associated Species of the Fusarium fujikuroi Species Complex Dewing, Claudette Van der Nest, Magrieta A. Santana, Quentin C. Proctor, Robert H. Wingfield, Brenda D. Steenkamp, Emma T. De Vos, Lieschen Pathogens Article The Fusarium fujikuroi species complex (FFSC) includes socioeconomically important pathogens that cause disease for numerous crops and synthesize a variety of secondary metabolites that can contaminate feedstocks and food. Here, we used comparative genomics to elucidate processes underlying the ability of pine-associated and grass-associated FFSC species to colonize tissues of their respective plant hosts. We characterized the identity, possible functions, evolutionary origins, and chromosomal positions of the host-range-associated genes encoded by the two groups of fungi. The 72 and 47 genes identified as unique to the respective genome groups were potentially involved in diverse processes, ranging from transcription, regulation, and substrate transport through to virulence/pathogenicity. Most genes arose early during the evolution of Fusarium/FFSC and were only subsequently retained in some lineages, while some had origins outside Fusarium. Although differences in the densities of these genes were especially noticeable on the conditionally dispensable chromosome of F. temperatum (representing the grass-associates) and F. circinatum (representing the pine-associates), the host-range-associated genes tended to be located towards the subtelomeric regions of chromosomes. Taken together, these results demonstrate that multiple mechanisms drive the emergence of genes in the grass- and pine-associated FFSC taxa examined. It also highlighted the diversity of the molecular processes potentially underlying niche-specificity in these and other Fusarium species. MDPI 2022-07-29 /pmc/articles/PMC9415769/ /pubmed/36014979 http://dx.doi.org/10.3390/pathogens11080858 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dewing, Claudette
Van der Nest, Magrieta A.
Santana, Quentin C.
Proctor, Robert H.
Wingfield, Brenda D.
Steenkamp, Emma T.
De Vos, Lieschen
Characterization of Host-Specific Genes from Pine- and Grass-Associated Species of the Fusarium fujikuroi Species Complex
title Characterization of Host-Specific Genes from Pine- and Grass-Associated Species of the Fusarium fujikuroi Species Complex
title_full Characterization of Host-Specific Genes from Pine- and Grass-Associated Species of the Fusarium fujikuroi Species Complex
title_fullStr Characterization of Host-Specific Genes from Pine- and Grass-Associated Species of the Fusarium fujikuroi Species Complex
title_full_unstemmed Characterization of Host-Specific Genes from Pine- and Grass-Associated Species of the Fusarium fujikuroi Species Complex
title_short Characterization of Host-Specific Genes from Pine- and Grass-Associated Species of the Fusarium fujikuroi Species Complex
title_sort characterization of host-specific genes from pine- and grass-associated species of the fusarium fujikuroi species complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415769/
https://www.ncbi.nlm.nih.gov/pubmed/36014979
http://dx.doi.org/10.3390/pathogens11080858
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