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Immunohistochemical Expression of Vitamin D Receptor in Uterine Fibroids

One of the many factors involved in the development of uterine fibroids is vitamin D deficiency. One aspect of this deficiency is decreased serum concentration of calcidiol-25(OH)D, a metabolite of D3 vitamin. The active form of vitamin D3, which arises after numerous enzymatic reactions, is calcitr...

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Autores principales: Markowska, Anna, Kurzawa, Paweł, Bednarek, Wiesława, Gryboś, Anna, Mardas, Marcin, Krzyżaniak, Monika, Majewski, Jan, Markowska, Janina, Gryboś, Marian, Żurawski, Jakub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415784/
https://www.ncbi.nlm.nih.gov/pubmed/36014877
http://dx.doi.org/10.3390/nu14163371
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author Markowska, Anna
Kurzawa, Paweł
Bednarek, Wiesława
Gryboś, Anna
Mardas, Marcin
Krzyżaniak, Monika
Majewski, Jan
Markowska, Janina
Gryboś, Marian
Żurawski, Jakub
author_facet Markowska, Anna
Kurzawa, Paweł
Bednarek, Wiesława
Gryboś, Anna
Mardas, Marcin
Krzyżaniak, Monika
Majewski, Jan
Markowska, Janina
Gryboś, Marian
Żurawski, Jakub
author_sort Markowska, Anna
collection PubMed
description One of the many factors involved in the development of uterine fibroids is vitamin D deficiency. One aspect of this deficiency is decreased serum concentration of calcidiol-25(OH)D, a metabolite of D3 vitamin. The active form of vitamin D3, which arises after numerous enzymatic reactions, is calcitriol-1,25(OH)2D3; this compound is transported to various body tissues. Vitamin D possesses extra-genomic effects due to its influence on various signaling pathways, i.e., through activating tyrosine kinases and by genomic effects via binding to a specific nuclear receptor, vitamin D receptor (VDR). The vitamin D/VDR complex regulates the expression of genes and is involved in the pathogenesis of fibroids. Numerous studies have shown that vitamin D supplementation reduces fibroid size. It has also been shown that the expression of VDR in myoma tissue is significantly lower than in the uterine muscle tissue at the tumor periphery. However, the expression of VDR in non-myoma uterine muscle has not previously been investigated. Our VDR expression studies were performed immunohistochemically with tissue microarrays (TMA) in three tissue groups: 98 uterine myoma tissues, 98 uterine tissues (tumor margin), and 12 tissues of normal uterine muscle (i.e., without fibroids). A statistical analysis showed significantly lower VDR expression in uterine muscle at the periphery of the fibroid than in healthy uterine muscle. Lower expression of VDR at the periphery of the myoma compared to that in normal uterine muscle may indicate potential for new myomas. This observation and the described reduction in the size of fibroids after vitamin D supplementation supports the hypothesis of causal development of uterine fibroids and may be useful for the prevention of re-development in the event of their excision from the uterus.
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spelling pubmed-94157842022-08-27 Immunohistochemical Expression of Vitamin D Receptor in Uterine Fibroids Markowska, Anna Kurzawa, Paweł Bednarek, Wiesława Gryboś, Anna Mardas, Marcin Krzyżaniak, Monika Majewski, Jan Markowska, Janina Gryboś, Marian Żurawski, Jakub Nutrients Article One of the many factors involved in the development of uterine fibroids is vitamin D deficiency. One aspect of this deficiency is decreased serum concentration of calcidiol-25(OH)D, a metabolite of D3 vitamin. The active form of vitamin D3, which arises after numerous enzymatic reactions, is calcitriol-1,25(OH)2D3; this compound is transported to various body tissues. Vitamin D possesses extra-genomic effects due to its influence on various signaling pathways, i.e., through activating tyrosine kinases and by genomic effects via binding to a specific nuclear receptor, vitamin D receptor (VDR). The vitamin D/VDR complex regulates the expression of genes and is involved in the pathogenesis of fibroids. Numerous studies have shown that vitamin D supplementation reduces fibroid size. It has also been shown that the expression of VDR in myoma tissue is significantly lower than in the uterine muscle tissue at the tumor periphery. However, the expression of VDR in non-myoma uterine muscle has not previously been investigated. Our VDR expression studies were performed immunohistochemically with tissue microarrays (TMA) in three tissue groups: 98 uterine myoma tissues, 98 uterine tissues (tumor margin), and 12 tissues of normal uterine muscle (i.e., without fibroids). A statistical analysis showed significantly lower VDR expression in uterine muscle at the periphery of the fibroid than in healthy uterine muscle. Lower expression of VDR at the periphery of the myoma compared to that in normal uterine muscle may indicate potential for new myomas. This observation and the described reduction in the size of fibroids after vitamin D supplementation supports the hypothesis of causal development of uterine fibroids and may be useful for the prevention of re-development in the event of their excision from the uterus. MDPI 2022-08-17 /pmc/articles/PMC9415784/ /pubmed/36014877 http://dx.doi.org/10.3390/nu14163371 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Markowska, Anna
Kurzawa, Paweł
Bednarek, Wiesława
Gryboś, Anna
Mardas, Marcin
Krzyżaniak, Monika
Majewski, Jan
Markowska, Janina
Gryboś, Marian
Żurawski, Jakub
Immunohistochemical Expression of Vitamin D Receptor in Uterine Fibroids
title Immunohistochemical Expression of Vitamin D Receptor in Uterine Fibroids
title_full Immunohistochemical Expression of Vitamin D Receptor in Uterine Fibroids
title_fullStr Immunohistochemical Expression of Vitamin D Receptor in Uterine Fibroids
title_full_unstemmed Immunohistochemical Expression of Vitamin D Receptor in Uterine Fibroids
title_short Immunohistochemical Expression of Vitamin D Receptor in Uterine Fibroids
title_sort immunohistochemical expression of vitamin d receptor in uterine fibroids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415784/
https://www.ncbi.nlm.nih.gov/pubmed/36014877
http://dx.doi.org/10.3390/nu14163371
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