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Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study
Polycaprolactone (PCL) is widely used in tissue engineering due to its interesting properties, namely biocompatibility, biodegradability, elastic nature, availability, cost efficacy, and the approval of health authorities such as the American Food and Drug Administration (FDA). The PCL degradation r...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415937/ https://www.ncbi.nlm.nih.gov/pubmed/36015652 http://dx.doi.org/10.3390/polym14163397 |
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author | Dias, Juliana R. Sousa, Aureliana Augusto, Ana Bártolo, Paulo J. Granja, Pedro L. |
author_facet | Dias, Juliana R. Sousa, Aureliana Augusto, Ana Bártolo, Paulo J. Granja, Pedro L. |
author_sort | Dias, Juliana R. |
collection | PubMed |
description | Polycaprolactone (PCL) is widely used in tissue engineering due to its interesting properties, namely biocompatibility, biodegradability, elastic nature, availability, cost efficacy, and the approval of health authorities such as the American Food and Drug Administration (FDA). The PCL degradation rate is not the most adequate for specific applications such as skin regeneration due to the hydrophobic nature of bulk PCL. However, PCL electrospun fiber meshes, due to their low diameters resulting in high surface area, are expected to exhibit a fast degradation rate. In this work, in vitro and in vivo degradation studies were performed over 90 days to evaluate the potential of electrospun PCL as a wound dressing. Enzymatic and hydrolytic degradation studies in vitro, performed in a static medium, demonstrated the influence of lipase, which promoted a rate of degradation of 97% for PCL meshes. In an in vivo scenario, the degradation was slower, although the samples were not rejected, and were well-integrated in the surrounding tissues inside the subcutaneous pockets specifically created. |
format | Online Article Text |
id | pubmed-9415937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94159372022-08-27 Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study Dias, Juliana R. Sousa, Aureliana Augusto, Ana Bártolo, Paulo J. Granja, Pedro L. Polymers (Basel) Article Polycaprolactone (PCL) is widely used in tissue engineering due to its interesting properties, namely biocompatibility, biodegradability, elastic nature, availability, cost efficacy, and the approval of health authorities such as the American Food and Drug Administration (FDA). The PCL degradation rate is not the most adequate for specific applications such as skin regeneration due to the hydrophobic nature of bulk PCL. However, PCL electrospun fiber meshes, due to their low diameters resulting in high surface area, are expected to exhibit a fast degradation rate. In this work, in vitro and in vivo degradation studies were performed over 90 days to evaluate the potential of electrospun PCL as a wound dressing. Enzymatic and hydrolytic degradation studies in vitro, performed in a static medium, demonstrated the influence of lipase, which promoted a rate of degradation of 97% for PCL meshes. In an in vivo scenario, the degradation was slower, although the samples were not rejected, and were well-integrated in the surrounding tissues inside the subcutaneous pockets specifically created. MDPI 2022-08-19 /pmc/articles/PMC9415937/ /pubmed/36015652 http://dx.doi.org/10.3390/polym14163397 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dias, Juliana R. Sousa, Aureliana Augusto, Ana Bártolo, Paulo J. Granja, Pedro L. Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study |
title | Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study |
title_full | Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study |
title_fullStr | Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study |
title_full_unstemmed | Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study |
title_short | Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study |
title_sort | electrospun polycaprolactone (pcl) degradation: an in vitro and in vivo study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415937/ https://www.ncbi.nlm.nih.gov/pubmed/36015652 http://dx.doi.org/10.3390/polym14163397 |
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