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Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study

Polycaprolactone (PCL) is widely used in tissue engineering due to its interesting properties, namely biocompatibility, biodegradability, elastic nature, availability, cost efficacy, and the approval of health authorities such as the American Food and Drug Administration (FDA). The PCL degradation r...

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Autores principales: Dias, Juliana R., Sousa, Aureliana, Augusto, Ana, Bártolo, Paulo J., Granja, Pedro L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415937/
https://www.ncbi.nlm.nih.gov/pubmed/36015652
http://dx.doi.org/10.3390/polym14163397
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author Dias, Juliana R.
Sousa, Aureliana
Augusto, Ana
Bártolo, Paulo J.
Granja, Pedro L.
author_facet Dias, Juliana R.
Sousa, Aureliana
Augusto, Ana
Bártolo, Paulo J.
Granja, Pedro L.
author_sort Dias, Juliana R.
collection PubMed
description Polycaprolactone (PCL) is widely used in tissue engineering due to its interesting properties, namely biocompatibility, biodegradability, elastic nature, availability, cost efficacy, and the approval of health authorities such as the American Food and Drug Administration (FDA). The PCL degradation rate is not the most adequate for specific applications such as skin regeneration due to the hydrophobic nature of bulk PCL. However, PCL electrospun fiber meshes, due to their low diameters resulting in high surface area, are expected to exhibit a fast degradation rate. In this work, in vitro and in vivo degradation studies were performed over 90 days to evaluate the potential of electrospun PCL as a wound dressing. Enzymatic and hydrolytic degradation studies in vitro, performed in a static medium, demonstrated the influence of lipase, which promoted a rate of degradation of 97% for PCL meshes. In an in vivo scenario, the degradation was slower, although the samples were not rejected, and were well-integrated in the surrounding tissues inside the subcutaneous pockets specifically created.
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spelling pubmed-94159372022-08-27 Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study Dias, Juliana R. Sousa, Aureliana Augusto, Ana Bártolo, Paulo J. Granja, Pedro L. Polymers (Basel) Article Polycaprolactone (PCL) is widely used in tissue engineering due to its interesting properties, namely biocompatibility, biodegradability, elastic nature, availability, cost efficacy, and the approval of health authorities such as the American Food and Drug Administration (FDA). The PCL degradation rate is not the most adequate for specific applications such as skin regeneration due to the hydrophobic nature of bulk PCL. However, PCL electrospun fiber meshes, due to their low diameters resulting in high surface area, are expected to exhibit a fast degradation rate. In this work, in vitro and in vivo degradation studies were performed over 90 days to evaluate the potential of electrospun PCL as a wound dressing. Enzymatic and hydrolytic degradation studies in vitro, performed in a static medium, demonstrated the influence of lipase, which promoted a rate of degradation of 97% for PCL meshes. In an in vivo scenario, the degradation was slower, although the samples were not rejected, and were well-integrated in the surrounding tissues inside the subcutaneous pockets specifically created. MDPI 2022-08-19 /pmc/articles/PMC9415937/ /pubmed/36015652 http://dx.doi.org/10.3390/polym14163397 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dias, Juliana R.
Sousa, Aureliana
Augusto, Ana
Bártolo, Paulo J.
Granja, Pedro L.
Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study
title Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study
title_full Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study
title_fullStr Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study
title_full_unstemmed Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study
title_short Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study
title_sort electrospun polycaprolactone (pcl) degradation: an in vitro and in vivo study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415937/
https://www.ncbi.nlm.nih.gov/pubmed/36015652
http://dx.doi.org/10.3390/polym14163397
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