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Using Self-Assembling ADDomer Platform to Display B and T Epitopes of Type O Foot-and-Mouth Disease Virus

Foot-and-mouth disease virus (FMDV) is a highly contagious and devastating virus that infects cloven-hoofed livestock and various wildlife species. Vaccination is the best measure to prevent FMD. ADDomer, as a kind of non-infectious adenovirus-inspired nanoparticle, has the advantage of high thermal...

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Autores principales: Luo, Chaowei, Yan, Quanhui, Huang, Juncong, Liu, Jiameng, Li, Yuwan, Wu, Keke, Li, Bingke, Zhao, Mingqiu, Fan, Shuangqi, Ding, Hongxing, Chen, Jinding
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416097/
https://www.ncbi.nlm.nih.gov/pubmed/36016432
http://dx.doi.org/10.3390/v14081810
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author Luo, Chaowei
Yan, Quanhui
Huang, Juncong
Liu, Jiameng
Li, Yuwan
Wu, Keke
Li, Bingke
Zhao, Mingqiu
Fan, Shuangqi
Ding, Hongxing
Chen, Jinding
author_facet Luo, Chaowei
Yan, Quanhui
Huang, Juncong
Liu, Jiameng
Li, Yuwan
Wu, Keke
Li, Bingke
Zhao, Mingqiu
Fan, Shuangqi
Ding, Hongxing
Chen, Jinding
author_sort Luo, Chaowei
collection PubMed
description Foot-and-mouth disease virus (FMDV) is a highly contagious and devastating virus that infects cloven-hoofed livestock and various wildlife species. Vaccination is the best measure to prevent FMD. ADDomer, as a kind of non-infectious adenovirus-inspired nanoparticle, has the advantage of high thermal stability. In this study, two dominant B-cell antigen epitopes (residues 129~160 and 200~213) and a dominant T-cell antigen epitope (residues 16~44) of type O FMDV were inserted into the ADDomer variable loop (VL) and arginine–glycine–aspartic acid (RGD) loop. The 3D structure of the recombinant protein (ADDomer-RBT) was simulated by homology modeling. First, the recombinant proteins were expressed by the baculovirus expression system and detected by western blot and Q Exactive mass spectrometry. Then the formation of VLPs was observed under a transmission electron micrograph (TEM). Finally, we evaluated the immunogenicity of chimeric VLPs with a murine model. Bioinformatic software analysis preliminarily corroborated that the chosen epitopes were successfully exposed on the surface of ADDomer VLPs. The TEM assay demonstrated the structural integrity of the VLPs. After immunizing, it was found that FMDV-specific antibodies can be produced in mice to induce humoral and cellular immune responses. To sum up, the ADDomer platform can be used as an effective antigen carrier to deliver antigen epitopes. This study presents one of the candidate vaccines to prevent and control FMDV.
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spelling pubmed-94160972022-08-27 Using Self-Assembling ADDomer Platform to Display B and T Epitopes of Type O Foot-and-Mouth Disease Virus Luo, Chaowei Yan, Quanhui Huang, Juncong Liu, Jiameng Li, Yuwan Wu, Keke Li, Bingke Zhao, Mingqiu Fan, Shuangqi Ding, Hongxing Chen, Jinding Viruses Article Foot-and-mouth disease virus (FMDV) is a highly contagious and devastating virus that infects cloven-hoofed livestock and various wildlife species. Vaccination is the best measure to prevent FMD. ADDomer, as a kind of non-infectious adenovirus-inspired nanoparticle, has the advantage of high thermal stability. In this study, two dominant B-cell antigen epitopes (residues 129~160 and 200~213) and a dominant T-cell antigen epitope (residues 16~44) of type O FMDV were inserted into the ADDomer variable loop (VL) and arginine–glycine–aspartic acid (RGD) loop. The 3D structure of the recombinant protein (ADDomer-RBT) was simulated by homology modeling. First, the recombinant proteins were expressed by the baculovirus expression system and detected by western blot and Q Exactive mass spectrometry. Then the formation of VLPs was observed under a transmission electron micrograph (TEM). Finally, we evaluated the immunogenicity of chimeric VLPs with a murine model. Bioinformatic software analysis preliminarily corroborated that the chosen epitopes were successfully exposed on the surface of ADDomer VLPs. The TEM assay demonstrated the structural integrity of the VLPs. After immunizing, it was found that FMDV-specific antibodies can be produced in mice to induce humoral and cellular immune responses. To sum up, the ADDomer platform can be used as an effective antigen carrier to deliver antigen epitopes. This study presents one of the candidate vaccines to prevent and control FMDV. MDPI 2022-08-18 /pmc/articles/PMC9416097/ /pubmed/36016432 http://dx.doi.org/10.3390/v14081810 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luo, Chaowei
Yan, Quanhui
Huang, Juncong
Liu, Jiameng
Li, Yuwan
Wu, Keke
Li, Bingke
Zhao, Mingqiu
Fan, Shuangqi
Ding, Hongxing
Chen, Jinding
Using Self-Assembling ADDomer Platform to Display B and T Epitopes of Type O Foot-and-Mouth Disease Virus
title Using Self-Assembling ADDomer Platform to Display B and T Epitopes of Type O Foot-and-Mouth Disease Virus
title_full Using Self-Assembling ADDomer Platform to Display B and T Epitopes of Type O Foot-and-Mouth Disease Virus
title_fullStr Using Self-Assembling ADDomer Platform to Display B and T Epitopes of Type O Foot-and-Mouth Disease Virus
title_full_unstemmed Using Self-Assembling ADDomer Platform to Display B and T Epitopes of Type O Foot-and-Mouth Disease Virus
title_short Using Self-Assembling ADDomer Platform to Display B and T Epitopes of Type O Foot-and-Mouth Disease Virus
title_sort using self-assembling addomer platform to display b and t epitopes of type o foot-and-mouth disease virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416097/
https://www.ncbi.nlm.nih.gov/pubmed/36016432
http://dx.doi.org/10.3390/v14081810
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