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TLC–Densitometry for Determination of Omeprazole in Simple and Combined Pharmaceutical Preparations

TLC combined with densitometry was used and chromatographic conditions developed to separate omeprazole and diclofenac sodium from their potential impurities. The development of the TLC–densitometry method is based on the elaboration of new chromatographic conditions allowing for the simultaneous de...

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Autores principales: Parys, Wioletta, Pyka-Pająk, Alina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416117/
https://www.ncbi.nlm.nih.gov/pubmed/36015164
http://dx.doi.org/10.3390/ph15081016
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author Parys, Wioletta
Pyka-Pająk, Alina
author_facet Parys, Wioletta
Pyka-Pająk, Alina
author_sort Parys, Wioletta
collection PubMed
description TLC combined with densitometry was used and chromatographic conditions developed to separate omeprazole and diclofenac sodium from their potential impurities. The development of the TLC–densitometry method is based on the elaboration of new chromatographic conditions allowing for the simultaneous determination of omeprazole and diclofenac sodium in a pharmaceutical preparation. Identification and quantification of omeprazole in simple and combined (with diclofenac) pharmaceutical preparations was performed on silica gel 60F(254) using one mobile phase: chloroform–methanol–ammonia (36:4:0.60, v/v). Diclofenac sodium was determined in the presence of omeprazole after 2D separation on silica gel using two mobile phases of the first phase of chloroform–methanol–ammonia (36:4:0.60, v/v) and the second mobile phase cyclohexane–chloroform–methanol–glacial acetic acid (6:3:0.5:0.5 v/v). The developed method is simple, economical, specific, precise, accurate, sensitive, and robust, with a good range of linearity for the quantification of omeprazole and diclofenac sodium. TLC in combination with densitometry can be used as an effective analytical tool for quality control and quantitative determination of omeprazole in simple and combined pharmaceutical preparations containing diclofenac sodium. TLC in combination with densitometry can be recommended for the analysis of omeprazole and diclofenac sodium in the absence of HPLC or spectrophotometer in the laboratory or to confirm results obtained with other analytical techniques.
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spelling pubmed-94161172022-08-27 TLC–Densitometry for Determination of Omeprazole in Simple and Combined Pharmaceutical Preparations Parys, Wioletta Pyka-Pająk, Alina Pharmaceuticals (Basel) Article TLC combined with densitometry was used and chromatographic conditions developed to separate omeprazole and diclofenac sodium from their potential impurities. The development of the TLC–densitometry method is based on the elaboration of new chromatographic conditions allowing for the simultaneous determination of omeprazole and diclofenac sodium in a pharmaceutical preparation. Identification and quantification of omeprazole in simple and combined (with diclofenac) pharmaceutical preparations was performed on silica gel 60F(254) using one mobile phase: chloroform–methanol–ammonia (36:4:0.60, v/v). Diclofenac sodium was determined in the presence of omeprazole after 2D separation on silica gel using two mobile phases of the first phase of chloroform–methanol–ammonia (36:4:0.60, v/v) and the second mobile phase cyclohexane–chloroform–methanol–glacial acetic acid (6:3:0.5:0.5 v/v). The developed method is simple, economical, specific, precise, accurate, sensitive, and robust, with a good range of linearity for the quantification of omeprazole and diclofenac sodium. TLC in combination with densitometry can be used as an effective analytical tool for quality control and quantitative determination of omeprazole in simple and combined pharmaceutical preparations containing diclofenac sodium. TLC in combination with densitometry can be recommended for the analysis of omeprazole and diclofenac sodium in the absence of HPLC or spectrophotometer in the laboratory or to confirm results obtained with other analytical techniques. MDPI 2022-08-18 /pmc/articles/PMC9416117/ /pubmed/36015164 http://dx.doi.org/10.3390/ph15081016 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parys, Wioletta
Pyka-Pająk, Alina
TLC–Densitometry for Determination of Omeprazole in Simple and Combined Pharmaceutical Preparations
title TLC–Densitometry for Determination of Omeprazole in Simple and Combined Pharmaceutical Preparations
title_full TLC–Densitometry for Determination of Omeprazole in Simple and Combined Pharmaceutical Preparations
title_fullStr TLC–Densitometry for Determination of Omeprazole in Simple and Combined Pharmaceutical Preparations
title_full_unstemmed TLC–Densitometry for Determination of Omeprazole in Simple and Combined Pharmaceutical Preparations
title_short TLC–Densitometry for Determination of Omeprazole in Simple and Combined Pharmaceutical Preparations
title_sort tlc–densitometry for determination of omeprazole in simple and combined pharmaceutical preparations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416117/
https://www.ncbi.nlm.nih.gov/pubmed/36015164
http://dx.doi.org/10.3390/ph15081016
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