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Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging

Nitrite (NO(2)(−)) is a common pollutant and is widely present in the environment and in human bodies. The development of a rapid and accurate method for NO(2)(−) detection is always a very important task. Herein, we synthesized a partnered excited-state intermolecular proton transfer (ESIPT) fluoro...

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Autores principales: Ma, Pan, Gong, Fuchun, Zhu, Hanming, Qian, You, He, Lingzhi, Xia, Jiaoyun, Cao, Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416243/
https://www.ncbi.nlm.nih.gov/pubmed/36014404
http://dx.doi.org/10.3390/molecules27165164
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author Ma, Pan
Gong, Fuchun
Zhu, Hanming
Qian, You
He, Lingzhi
Xia, Jiaoyun
Cao, Zhong
author_facet Ma, Pan
Gong, Fuchun
Zhu, Hanming
Qian, You
He, Lingzhi
Xia, Jiaoyun
Cao, Zhong
author_sort Ma, Pan
collection PubMed
description Nitrite (NO(2)(−)) is a common pollutant and is widely present in the environment and in human bodies. The development of a rapid and accurate method for NO(2)(−) detection is always a very important task. Herein, we synthesized a partnered excited-state intermolecular proton transfer (ESIPT) fluorophore using the “multi-component one pot” method, and used this as a probe (ESIPT-F) for sensing NO(2)(−). ESIPT-F exhibited bimodal emission in different solvents because of the solvent-mediated ESIPT reaction. The addition of NO(2)(−) caused an obvious change in colors and tautomeric fluorescence due to the graft of NO(2)(−) into the ESIPT-F molecules. From this basis, highly sensitive and selective analysis of NO(2)(−) was developed using tautomeric emission signaling, achieving sensitive detection of NO(2)(−) in the concentration range of 0~45 mM with a detection limit of 12.5 nM. More importantly, ESIPT-F showed the ability to anchor proteins and resulted in a recognition-driven “on-off” ESIPT process, enabling it to become a powerful tool for fluorescence imaging of proteins or protein-based subcellular organelles. MTT experimental results revealed that ESIPT-F is low cytotoxic and has good membrane permeability to cells. Thus, ESIPT-F was further employed to image the tunneling nanotube in vitro HEC-1A cells, displaying high-resolution performance.
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spelling pubmed-94162432022-08-27 Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging Ma, Pan Gong, Fuchun Zhu, Hanming Qian, You He, Lingzhi Xia, Jiaoyun Cao, Zhong Molecules Article Nitrite (NO(2)(−)) is a common pollutant and is widely present in the environment and in human bodies. The development of a rapid and accurate method for NO(2)(−) detection is always a very important task. Herein, we synthesized a partnered excited-state intermolecular proton transfer (ESIPT) fluorophore using the “multi-component one pot” method, and used this as a probe (ESIPT-F) for sensing NO(2)(−). ESIPT-F exhibited bimodal emission in different solvents because of the solvent-mediated ESIPT reaction. The addition of NO(2)(−) caused an obvious change in colors and tautomeric fluorescence due to the graft of NO(2)(−) into the ESIPT-F molecules. From this basis, highly sensitive and selective analysis of NO(2)(−) was developed using tautomeric emission signaling, achieving sensitive detection of NO(2)(−) in the concentration range of 0~45 mM with a detection limit of 12.5 nM. More importantly, ESIPT-F showed the ability to anchor proteins and resulted in a recognition-driven “on-off” ESIPT process, enabling it to become a powerful tool for fluorescence imaging of proteins or protein-based subcellular organelles. MTT experimental results revealed that ESIPT-F is low cytotoxic and has good membrane permeability to cells. Thus, ESIPT-F was further employed to image the tunneling nanotube in vitro HEC-1A cells, displaying high-resolution performance. MDPI 2022-08-13 /pmc/articles/PMC9416243/ /pubmed/36014404 http://dx.doi.org/10.3390/molecules27165164 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Pan
Gong, Fuchun
Zhu, Hanming
Qian, You
He, Lingzhi
Xia, Jiaoyun
Cao, Zhong
Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging
title Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging
title_full Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging
title_fullStr Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging
title_full_unstemmed Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging
title_short Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging
title_sort partnered excited-state intermolecular proton transfer fluorescence (p-esipt) signaling for nitrate sensing and high-resolution cell-imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416243/
https://www.ncbi.nlm.nih.gov/pubmed/36014404
http://dx.doi.org/10.3390/molecules27165164
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