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Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging
Nitrite (NO(2)(−)) is a common pollutant and is widely present in the environment and in human bodies. The development of a rapid and accurate method for NO(2)(−) detection is always a very important task. Herein, we synthesized a partnered excited-state intermolecular proton transfer (ESIPT) fluoro...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416243/ https://www.ncbi.nlm.nih.gov/pubmed/36014404 http://dx.doi.org/10.3390/molecules27165164 |
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author | Ma, Pan Gong, Fuchun Zhu, Hanming Qian, You He, Lingzhi Xia, Jiaoyun Cao, Zhong |
author_facet | Ma, Pan Gong, Fuchun Zhu, Hanming Qian, You He, Lingzhi Xia, Jiaoyun Cao, Zhong |
author_sort | Ma, Pan |
collection | PubMed |
description | Nitrite (NO(2)(−)) is a common pollutant and is widely present in the environment and in human bodies. The development of a rapid and accurate method for NO(2)(−) detection is always a very important task. Herein, we synthesized a partnered excited-state intermolecular proton transfer (ESIPT) fluorophore using the “multi-component one pot” method, and used this as a probe (ESIPT-F) for sensing NO(2)(−). ESIPT-F exhibited bimodal emission in different solvents because of the solvent-mediated ESIPT reaction. The addition of NO(2)(−) caused an obvious change in colors and tautomeric fluorescence due to the graft of NO(2)(−) into the ESIPT-F molecules. From this basis, highly sensitive and selective analysis of NO(2)(−) was developed using tautomeric emission signaling, achieving sensitive detection of NO(2)(−) in the concentration range of 0~45 mM with a detection limit of 12.5 nM. More importantly, ESIPT-F showed the ability to anchor proteins and resulted in a recognition-driven “on-off” ESIPT process, enabling it to become a powerful tool for fluorescence imaging of proteins or protein-based subcellular organelles. MTT experimental results revealed that ESIPT-F is low cytotoxic and has good membrane permeability to cells. Thus, ESIPT-F was further employed to image the tunneling nanotube in vitro HEC-1A cells, displaying high-resolution performance. |
format | Online Article Text |
id | pubmed-9416243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94162432022-08-27 Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging Ma, Pan Gong, Fuchun Zhu, Hanming Qian, You He, Lingzhi Xia, Jiaoyun Cao, Zhong Molecules Article Nitrite (NO(2)(−)) is a common pollutant and is widely present in the environment and in human bodies. The development of a rapid and accurate method for NO(2)(−) detection is always a very important task. Herein, we synthesized a partnered excited-state intermolecular proton transfer (ESIPT) fluorophore using the “multi-component one pot” method, and used this as a probe (ESIPT-F) for sensing NO(2)(−). ESIPT-F exhibited bimodal emission in different solvents because of the solvent-mediated ESIPT reaction. The addition of NO(2)(−) caused an obvious change in colors and tautomeric fluorescence due to the graft of NO(2)(−) into the ESIPT-F molecules. From this basis, highly sensitive and selective analysis of NO(2)(−) was developed using tautomeric emission signaling, achieving sensitive detection of NO(2)(−) in the concentration range of 0~45 mM with a detection limit of 12.5 nM. More importantly, ESIPT-F showed the ability to anchor proteins and resulted in a recognition-driven “on-off” ESIPT process, enabling it to become a powerful tool for fluorescence imaging of proteins or protein-based subcellular organelles. MTT experimental results revealed that ESIPT-F is low cytotoxic and has good membrane permeability to cells. Thus, ESIPT-F was further employed to image the tunneling nanotube in vitro HEC-1A cells, displaying high-resolution performance. MDPI 2022-08-13 /pmc/articles/PMC9416243/ /pubmed/36014404 http://dx.doi.org/10.3390/molecules27165164 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ma, Pan Gong, Fuchun Zhu, Hanming Qian, You He, Lingzhi Xia, Jiaoyun Cao, Zhong Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging |
title | Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging |
title_full | Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging |
title_fullStr | Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging |
title_full_unstemmed | Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging |
title_short | Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging |
title_sort | partnered excited-state intermolecular proton transfer fluorescence (p-esipt) signaling for nitrate sensing and high-resolution cell-imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416243/ https://www.ncbi.nlm.nih.gov/pubmed/36014404 http://dx.doi.org/10.3390/molecules27165164 |
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