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Development and Evaluation of an Antimicrobial Formulation Containing Rosmarinus officinalis
Rosmarinus officinalis belongs to the Lamiaceae family, and its constituents show antioxidant, anti-inflammatory, antidepressant, antinociceptive, and antibacterial properties. The aim of this study was to develop a topical formulation with R. officinalis extract that had antimicrobial and antioxida...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416300/ https://www.ncbi.nlm.nih.gov/pubmed/36014289 http://dx.doi.org/10.3390/molecules27165049 |
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author | de Macedo, Lucas Malvezzi dos Santos, Érica Mendes Ataide, Janaína Artem Silva, Gabriela Trindade de Souza e Guarnieri, João Paulo de Oliveira Lancellotti, Marcelo Jozala, Angela Faustino Rosa, Paulo Cesar Pires Mazzola, Priscila Gava |
author_facet | de Macedo, Lucas Malvezzi dos Santos, Érica Mendes Ataide, Janaína Artem Silva, Gabriela Trindade de Souza e Guarnieri, João Paulo de Oliveira Lancellotti, Marcelo Jozala, Angela Faustino Rosa, Paulo Cesar Pires Mazzola, Priscila Gava |
author_sort | de Macedo, Lucas Malvezzi |
collection | PubMed |
description | Rosmarinus officinalis belongs to the Lamiaceae family, and its constituents show antioxidant, anti-inflammatory, antidepressant, antinociceptive, and antibacterial properties. The aim of this study was to develop a topical formulation with R. officinalis extract that had antimicrobial and antioxidant activity. Maceration, infusion, Soxhlet, and ultrasound were used to produce rosemary extracts, which were submitted to antioxidant, compound quantification, cell viability, and antimicrobial assays. Infusion and Soxhlet showed better results in the DPPH assay. During compound quantification, infusion showed promising metabolite extraction in phenolic compounds and tannins, although maceration was able to extract more flavonoids. The infusion and ultrasound extracts affected more strains of skin bacteria in the disk diffusion assays. In the minimum inhibitory concentration assay, the infusion extract showed results against S. aureus, S. oralis, and P. aeruginosa, while ultrasound showed effects against those three bacteria and E. coli. The infusion extract was chosen to be incorporated into a green emulsion. The infusion extract promoted lower spreadability and appropriated the texture, and the blank formulation showed high levels of acceptance among the volunteers. According to the results, the rosemary extract showed promising antioxidant and antimicrobial activity, and the developed formulations containing this extract were stable for over 90 days and had acceptable characteristics, suggesting its potential use as a phytocosmetic. This paper reports the first attempt to produce an oil-in-water emulsion using only natural excipients and rosemary extract, which is a promising novelty, as similar products cannot be found on the market or in the scientific literature. |
format | Online Article Text |
id | pubmed-9416300 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94163002022-08-27 Development and Evaluation of an Antimicrobial Formulation Containing Rosmarinus officinalis de Macedo, Lucas Malvezzi dos Santos, Érica Mendes Ataide, Janaína Artem Silva, Gabriela Trindade de Souza e Guarnieri, João Paulo de Oliveira Lancellotti, Marcelo Jozala, Angela Faustino Rosa, Paulo Cesar Pires Mazzola, Priscila Gava Molecules Article Rosmarinus officinalis belongs to the Lamiaceae family, and its constituents show antioxidant, anti-inflammatory, antidepressant, antinociceptive, and antibacterial properties. The aim of this study was to develop a topical formulation with R. officinalis extract that had antimicrobial and antioxidant activity. Maceration, infusion, Soxhlet, and ultrasound were used to produce rosemary extracts, which were submitted to antioxidant, compound quantification, cell viability, and antimicrobial assays. Infusion and Soxhlet showed better results in the DPPH assay. During compound quantification, infusion showed promising metabolite extraction in phenolic compounds and tannins, although maceration was able to extract more flavonoids. The infusion and ultrasound extracts affected more strains of skin bacteria in the disk diffusion assays. In the minimum inhibitory concentration assay, the infusion extract showed results against S. aureus, S. oralis, and P. aeruginosa, while ultrasound showed effects against those three bacteria and E. coli. The infusion extract was chosen to be incorporated into a green emulsion. The infusion extract promoted lower spreadability and appropriated the texture, and the blank formulation showed high levels of acceptance among the volunteers. According to the results, the rosemary extract showed promising antioxidant and antimicrobial activity, and the developed formulations containing this extract were stable for over 90 days and had acceptable characteristics, suggesting its potential use as a phytocosmetic. This paper reports the first attempt to produce an oil-in-water emulsion using only natural excipients and rosemary extract, which is a promising novelty, as similar products cannot be found on the market or in the scientific literature. MDPI 2022-08-09 /pmc/articles/PMC9416300/ /pubmed/36014289 http://dx.doi.org/10.3390/molecules27165049 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Macedo, Lucas Malvezzi dos Santos, Érica Mendes Ataide, Janaína Artem Silva, Gabriela Trindade de Souza e Guarnieri, João Paulo de Oliveira Lancellotti, Marcelo Jozala, Angela Faustino Rosa, Paulo Cesar Pires Mazzola, Priscila Gava Development and Evaluation of an Antimicrobial Formulation Containing Rosmarinus officinalis |
title | Development and Evaluation of an Antimicrobial Formulation Containing Rosmarinus officinalis |
title_full | Development and Evaluation of an Antimicrobial Formulation Containing Rosmarinus officinalis |
title_fullStr | Development and Evaluation of an Antimicrobial Formulation Containing Rosmarinus officinalis |
title_full_unstemmed | Development and Evaluation of an Antimicrobial Formulation Containing Rosmarinus officinalis |
title_short | Development and Evaluation of an Antimicrobial Formulation Containing Rosmarinus officinalis |
title_sort | development and evaluation of an antimicrobial formulation containing rosmarinus officinalis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416300/ https://www.ncbi.nlm.nih.gov/pubmed/36014289 http://dx.doi.org/10.3390/molecules27165049 |
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