Cargando…

A Portable ‘Plug-and-Play’ Fibre Optic Sensor for In-Situ Measurements of pH Values for Microfluidic Applications

Microfluidics is used in many applications ranging from chemistry, medicine, biology and biomedical research, and the ability to measure pH values in-situ is an important parameter for creating and monitoring environments within a microfluidic chip for many such applications. We present a portable,...

Descripción completa

Detalles Bibliográficos
Autores principales: Kumar, Rahul, Nguyen, Hien, Rente, Bruno, Tan, Christabel, Sun, Tong, Grattan, Kenneth T. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416338/
https://www.ncbi.nlm.nih.gov/pubmed/36014146
http://dx.doi.org/10.3390/mi13081224
_version_ 1784776455700348928
author Kumar, Rahul
Nguyen, Hien
Rente, Bruno
Tan, Christabel
Sun, Tong
Grattan, Kenneth T. V.
author_facet Kumar, Rahul
Nguyen, Hien
Rente, Bruno
Tan, Christabel
Sun, Tong
Grattan, Kenneth T. V.
author_sort Kumar, Rahul
collection PubMed
description Microfluidics is used in many applications ranging from chemistry, medicine, biology and biomedical research, and the ability to measure pH values in-situ is an important parameter for creating and monitoring environments within a microfluidic chip for many such applications. We present a portable, optical fibre-based sensor for monitoring the pH based on the fluorescent intensity change of an acrylamidofluorescein dye, immobilized on the tip of a multimode optical fibre, and its performance is evaluated in-situ in a microfluidic channel. The sensor showed a sigmoid response over the pH range of 6.0–8.5, with a maximum sensitivity of 0.2/pH in the mid-range at pH 7.5. Following its evaluation, the sensor developed was used in a single microfluidic PDMS channel and its response was monitored for various flow rates within the channel. The results thus obtained showed that the sensor is sufficiently robust and well-suited to be used for measuring the pH value of the flowing liquid in the microchannel, allowing it to be used for a number of practical applications in ‘lab-on-a-chip’ applications where microfluidics are used. A key feature of the sensor is its simplicity and the ease of integrating the sensor with the microfluidic channel being probed.
format Online
Article
Text
id pubmed-9416338
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94163382022-08-27 A Portable ‘Plug-and-Play’ Fibre Optic Sensor for In-Situ Measurements of pH Values for Microfluidic Applications Kumar, Rahul Nguyen, Hien Rente, Bruno Tan, Christabel Sun, Tong Grattan, Kenneth T. V. Micromachines (Basel) Article Microfluidics is used in many applications ranging from chemistry, medicine, biology and biomedical research, and the ability to measure pH values in-situ is an important parameter for creating and monitoring environments within a microfluidic chip for many such applications. We present a portable, optical fibre-based sensor for monitoring the pH based on the fluorescent intensity change of an acrylamidofluorescein dye, immobilized on the tip of a multimode optical fibre, and its performance is evaluated in-situ in a microfluidic channel. The sensor showed a sigmoid response over the pH range of 6.0–8.5, with a maximum sensitivity of 0.2/pH in the mid-range at pH 7.5. Following its evaluation, the sensor developed was used in a single microfluidic PDMS channel and its response was monitored for various flow rates within the channel. The results thus obtained showed that the sensor is sufficiently robust and well-suited to be used for measuring the pH value of the flowing liquid in the microchannel, allowing it to be used for a number of practical applications in ‘lab-on-a-chip’ applications where microfluidics are used. A key feature of the sensor is its simplicity and the ease of integrating the sensor with the microfluidic channel being probed. MDPI 2022-07-30 /pmc/articles/PMC9416338/ /pubmed/36014146 http://dx.doi.org/10.3390/mi13081224 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kumar, Rahul
Nguyen, Hien
Rente, Bruno
Tan, Christabel
Sun, Tong
Grattan, Kenneth T. V.
A Portable ‘Plug-and-Play’ Fibre Optic Sensor for In-Situ Measurements of pH Values for Microfluidic Applications
title A Portable ‘Plug-and-Play’ Fibre Optic Sensor for In-Situ Measurements of pH Values for Microfluidic Applications
title_full A Portable ‘Plug-and-Play’ Fibre Optic Sensor for In-Situ Measurements of pH Values for Microfluidic Applications
title_fullStr A Portable ‘Plug-and-Play’ Fibre Optic Sensor for In-Situ Measurements of pH Values for Microfluidic Applications
title_full_unstemmed A Portable ‘Plug-and-Play’ Fibre Optic Sensor for In-Situ Measurements of pH Values for Microfluidic Applications
title_short A Portable ‘Plug-and-Play’ Fibre Optic Sensor for In-Situ Measurements of pH Values for Microfluidic Applications
title_sort portable ‘plug-and-play’ fibre optic sensor for in-situ measurements of ph values for microfluidic applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416338/
https://www.ncbi.nlm.nih.gov/pubmed/36014146
http://dx.doi.org/10.3390/mi13081224
work_keys_str_mv AT kumarrahul aportableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT nguyenhien aportableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT rentebruno aportableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT tanchristabel aportableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT suntong aportableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT grattankennethtv aportableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT kumarrahul portableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT nguyenhien portableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT rentebruno portableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT tanchristabel portableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT suntong portableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications
AT grattankennethtv portableplugandplayfibreopticsensorforinsitumeasurementsofphvaluesformicrofluidicapplications