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Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus

Vibrio alginolyticus is an important pathogen of marine animals and has been the target of phage therapy applications in marine aquaculture for many years. Here, we report the isolation and partial characterization of a novel species of the Siphoviridae family, the Vibrio phage Artemius. The novel p...

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Autores principales: Droubogiannis, Stavros, Pavlidi, Lydia, Tsertou, Maria Ioanna, Kokkari, Constantina, Skliros, Dimitrios, Flemetakis, Emmanouil, Katharios, Pantelis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416449/
https://www.ncbi.nlm.nih.gov/pubmed/36014969
http://dx.doi.org/10.3390/pathogens11080848
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author Droubogiannis, Stavros
Pavlidi, Lydia
Tsertou, Maria Ioanna
Kokkari, Constantina
Skliros, Dimitrios
Flemetakis, Emmanouil
Katharios, Pantelis
author_facet Droubogiannis, Stavros
Pavlidi, Lydia
Tsertou, Maria Ioanna
Kokkari, Constantina
Skliros, Dimitrios
Flemetakis, Emmanouil
Katharios, Pantelis
author_sort Droubogiannis, Stavros
collection PubMed
description Vibrio alginolyticus is an important pathogen of marine animals and has been the target of phage therapy applications in marine aquaculture for many years. Here, we report the isolation and partial characterization of a novel species of the Siphoviridae family, the Vibrio phage Artemius. The novel phage was species-specific and could only infect strains of V. alginolyticus. It could efficiently reduce the growth of the host bacterium at various multiplicities of infection as assessed by an in vitro lysis assay. It had a genome length of 43,349 base pairs. The complete genome has double-stranded DNA with a G + C content of 43.61%. In total, 57 ORFs were identified, of which 19 were assigned a predicted function. A genomic analysis indicated that Vibrio phage Artemius is lytic and does not harbor genes encoding toxins and antibiotic resistance determinants.
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spelling pubmed-94164492022-08-27 Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus Droubogiannis, Stavros Pavlidi, Lydia Tsertou, Maria Ioanna Kokkari, Constantina Skliros, Dimitrios Flemetakis, Emmanouil Katharios, Pantelis Pathogens Article Vibrio alginolyticus is an important pathogen of marine animals and has been the target of phage therapy applications in marine aquaculture for many years. Here, we report the isolation and partial characterization of a novel species of the Siphoviridae family, the Vibrio phage Artemius. The novel phage was species-specific and could only infect strains of V. alginolyticus. It could efficiently reduce the growth of the host bacterium at various multiplicities of infection as assessed by an in vitro lysis assay. It had a genome length of 43,349 base pairs. The complete genome has double-stranded DNA with a G + C content of 43.61%. In total, 57 ORFs were identified, of which 19 were assigned a predicted function. A genomic analysis indicated that Vibrio phage Artemius is lytic and does not harbor genes encoding toxins and antibiotic resistance determinants. MDPI 2022-07-28 /pmc/articles/PMC9416449/ /pubmed/36014969 http://dx.doi.org/10.3390/pathogens11080848 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Droubogiannis, Stavros
Pavlidi, Lydia
Tsertou, Maria Ioanna
Kokkari, Constantina
Skliros, Dimitrios
Flemetakis, Emmanouil
Katharios, Pantelis
Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus
title Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus
title_full Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus
title_fullStr Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus
title_full_unstemmed Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus
title_short Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus
title_sort vibrio phage artemius, a novel phage infecting vibrio alginolyticus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416449/
https://www.ncbi.nlm.nih.gov/pubmed/36014969
http://dx.doi.org/10.3390/pathogens11080848
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