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Assays for Identification and Differentiation of Brucella Species: A Review

Brucellosis is one of the most important and widespread bacterial zoonoses worldwide. Cases are reported annually across the range of known infectious species of the genus Brucella. Globally, Brucella melitensis, primarily hosted by domestic sheep and goats, affects large proportions of livestock he...

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Autores principales: Kurmanov, Berzhan, Zincke, Diansy, Su, Wanwen, Hadfield, Ted L., Aikimbayev, Alim, Karibayev, Talgat, Berdikulov, Maxat, Orynbayev, Mukhit, Nikolich, Mikeljon P., Blackburn, Jason K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416531/
https://www.ncbi.nlm.nih.gov/pubmed/36014002
http://dx.doi.org/10.3390/microorganisms10081584
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author Kurmanov, Berzhan
Zincke, Diansy
Su, Wanwen
Hadfield, Ted L.
Aikimbayev, Alim
Karibayev, Talgat
Berdikulov, Maxat
Orynbayev, Mukhit
Nikolich, Mikeljon P.
Blackburn, Jason K.
author_facet Kurmanov, Berzhan
Zincke, Diansy
Su, Wanwen
Hadfield, Ted L.
Aikimbayev, Alim
Karibayev, Talgat
Berdikulov, Maxat
Orynbayev, Mukhit
Nikolich, Mikeljon P.
Blackburn, Jason K.
author_sort Kurmanov, Berzhan
collection PubMed
description Brucellosis is one of the most important and widespread bacterial zoonoses worldwide. Cases are reported annually across the range of known infectious species of the genus Brucella. Globally, Brucella melitensis, primarily hosted by domestic sheep and goats, affects large proportions of livestock herds, and frequently spills over into humans. While some species, such as Brucella abortus, are well controlled in livestock in areas of North America, the Greater Yellowstone Ecosystem supports the species in native wild ungulates with occasional spillover to livestock. Elsewhere in North America, other Brucella species still infect domestic dogs and feral swine, with some associated human cases. Brucella spp. patterns vary across space globally with B. abortus and B. melitensis the most important for livestock control. A myriad of other species within the genus infect a wide range of marine mammals, wildlife, rodents, and even frogs. Infection in humans from these others varies with geography and bacterial species. Control in humans is primarily achieved through livestock vaccination and culling and requires accurate and rapid species confirmation; vaccination is Brucella spp.-specific and typically targets single livestock species for distribution. Traditional bacteriology methods are slow (some media can take up to 21 days for bacterial growth) and often lack the specificity of molecular techniques. Here, we summarize the molecular techniques for confirming and identifying specific Brucella species and provide recommendations for selecting the appropriate methods based on need, sensitivity, and laboratory capabilities/technology. As vaccination/culling approaches are costly and logistically challenging, proper diagnostics and species identification are critical tools for targeting surveillance and control.
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spelling pubmed-94165312022-08-27 Assays for Identification and Differentiation of Brucella Species: A Review Kurmanov, Berzhan Zincke, Diansy Su, Wanwen Hadfield, Ted L. Aikimbayev, Alim Karibayev, Talgat Berdikulov, Maxat Orynbayev, Mukhit Nikolich, Mikeljon P. Blackburn, Jason K. Microorganisms Review Brucellosis is one of the most important and widespread bacterial zoonoses worldwide. Cases are reported annually across the range of known infectious species of the genus Brucella. Globally, Brucella melitensis, primarily hosted by domestic sheep and goats, affects large proportions of livestock herds, and frequently spills over into humans. While some species, such as Brucella abortus, are well controlled in livestock in areas of North America, the Greater Yellowstone Ecosystem supports the species in native wild ungulates with occasional spillover to livestock. Elsewhere in North America, other Brucella species still infect domestic dogs and feral swine, with some associated human cases. Brucella spp. patterns vary across space globally with B. abortus and B. melitensis the most important for livestock control. A myriad of other species within the genus infect a wide range of marine mammals, wildlife, rodents, and even frogs. Infection in humans from these others varies with geography and bacterial species. Control in humans is primarily achieved through livestock vaccination and culling and requires accurate and rapid species confirmation; vaccination is Brucella spp.-specific and typically targets single livestock species for distribution. Traditional bacteriology methods are slow (some media can take up to 21 days for bacterial growth) and often lack the specificity of molecular techniques. Here, we summarize the molecular techniques for confirming and identifying specific Brucella species and provide recommendations for selecting the appropriate methods based on need, sensitivity, and laboratory capabilities/technology. As vaccination/culling approaches are costly and logistically challenging, proper diagnostics and species identification are critical tools for targeting surveillance and control. MDPI 2022-08-06 /pmc/articles/PMC9416531/ /pubmed/36014002 http://dx.doi.org/10.3390/microorganisms10081584 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kurmanov, Berzhan
Zincke, Diansy
Su, Wanwen
Hadfield, Ted L.
Aikimbayev, Alim
Karibayev, Talgat
Berdikulov, Maxat
Orynbayev, Mukhit
Nikolich, Mikeljon P.
Blackburn, Jason K.
Assays for Identification and Differentiation of Brucella Species: A Review
title Assays for Identification and Differentiation of Brucella Species: A Review
title_full Assays for Identification and Differentiation of Brucella Species: A Review
title_fullStr Assays for Identification and Differentiation of Brucella Species: A Review
title_full_unstemmed Assays for Identification and Differentiation of Brucella Species: A Review
title_short Assays for Identification and Differentiation of Brucella Species: A Review
title_sort assays for identification and differentiation of brucella species: a review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416531/
https://www.ncbi.nlm.nih.gov/pubmed/36014002
http://dx.doi.org/10.3390/microorganisms10081584
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