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Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging to in vivo imaging of tumours

Tailor-made NIR emitting dyes were designed as multimodal optical probes. These asymmetric amphiphilic compounds show combined intense absorption in the visible region, NIR fluorescence emission, high two-photon absorption in the NIR (with the maximum located around 1000 nm) as well as large Stokes&...

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Autores principales: Prévot, Geoffrey, Bsaibess, Talia, Daniel, Jonathan, Genevois, Coralie, Clermont, Guillaume, Sasaki, Isabelle, Marais, Sebastien, Couillaud, Franck, Crauste-Manciet, Sylvie, Blanchard-Desce, Mireille
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416932/
https://www.ncbi.nlm.nih.gov/pubmed/36132308
http://dx.doi.org/10.1039/c9na00710e
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author Prévot, Geoffrey
Bsaibess, Talia
Daniel, Jonathan
Genevois, Coralie
Clermont, Guillaume
Sasaki, Isabelle
Marais, Sebastien
Couillaud, Franck
Crauste-Manciet, Sylvie
Blanchard-Desce, Mireille
author_facet Prévot, Geoffrey
Bsaibess, Talia
Daniel, Jonathan
Genevois, Coralie
Clermont, Guillaume
Sasaki, Isabelle
Marais, Sebastien
Couillaud, Franck
Crauste-Manciet, Sylvie
Blanchard-Desce, Mireille
author_sort Prévot, Geoffrey
collection PubMed
description Tailor-made NIR emitting dyes were designed as multimodal optical probes. These asymmetric amphiphilic compounds show combined intense absorption in the visible region, NIR fluorescence emission, high two-photon absorption in the NIR (with the maximum located around 1000 nm) as well as large Stokes' shift values and second-harmonic generation ability. Thanks to their structure, high loading into nanoemulsions (NEs) could be achieved leading to very high one- and two-photon brightness. These dyes were demonstrated to act as multimodal contrast agents able to generate different optical modalities of interest for bioimaging. Indeed, the uptake and carrier behaviour of the dye-loaded NEs into cancer cells could be monitored by simultaneous two-photon fluorescence and second-harmonic generation optical imaging. Multimodal imaging provided deep insight into the mechanism and kinetics of dye internalisation. Quite interestingly, the nature of the dyes was also found to influence both the kinetics of endocytosis and the internalisation pathways in glioblastoma cancer cells. By modulating the charge distribution within the dyes, the NEs can be tuned to escape lysosomes and enter the mitochondria. Moreover, surface functionalization with PEG macromolecules was realized to yield stealth NIRF-NEs which could be used for in vivo NIRF imaging of subcutaneous tumours in mice.
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spelling pubmed-94169322022-09-20 Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging to in vivo imaging of tumours Prévot, Geoffrey Bsaibess, Talia Daniel, Jonathan Genevois, Coralie Clermont, Guillaume Sasaki, Isabelle Marais, Sebastien Couillaud, Franck Crauste-Manciet, Sylvie Blanchard-Desce, Mireille Nanoscale Adv Chemistry Tailor-made NIR emitting dyes were designed as multimodal optical probes. These asymmetric amphiphilic compounds show combined intense absorption in the visible region, NIR fluorescence emission, high two-photon absorption in the NIR (with the maximum located around 1000 nm) as well as large Stokes' shift values and second-harmonic generation ability. Thanks to their structure, high loading into nanoemulsions (NEs) could be achieved leading to very high one- and two-photon brightness. These dyes were demonstrated to act as multimodal contrast agents able to generate different optical modalities of interest for bioimaging. Indeed, the uptake and carrier behaviour of the dye-loaded NEs into cancer cells could be monitored by simultaneous two-photon fluorescence and second-harmonic generation optical imaging. Multimodal imaging provided deep insight into the mechanism and kinetics of dye internalisation. Quite interestingly, the nature of the dyes was also found to influence both the kinetics of endocytosis and the internalisation pathways in glioblastoma cancer cells. By modulating the charge distribution within the dyes, the NEs can be tuned to escape lysosomes and enter the mitochondria. Moreover, surface functionalization with PEG macromolecules was realized to yield stealth NIRF-NEs which could be used for in vivo NIRF imaging of subcutaneous tumours in mice. RSC 2020-02-27 /pmc/articles/PMC9416932/ /pubmed/36132308 http://dx.doi.org/10.1039/c9na00710e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Prévot, Geoffrey
Bsaibess, Talia
Daniel, Jonathan
Genevois, Coralie
Clermont, Guillaume
Sasaki, Isabelle
Marais, Sebastien
Couillaud, Franck
Crauste-Manciet, Sylvie
Blanchard-Desce, Mireille
Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging to in vivo imaging of tumours
title Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging to in vivo imaging of tumours
title_full Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging to in vivo imaging of tumours
title_fullStr Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging to in vivo imaging of tumours
title_full_unstemmed Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging to in vivo imaging of tumours
title_short Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging to in vivo imaging of tumours
title_sort multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. from live cell imaging to in vivo imaging of tumours
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416932/
https://www.ncbi.nlm.nih.gov/pubmed/36132308
http://dx.doi.org/10.1039/c9na00710e
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