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High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI
There is a need for biofunctionalised magnetic nanoparticles for many biomedical applications, including MRI contrast agents that have a range of surface properties and functional groups. A library of eleven adducts, each formed by condensing a reducing sugar with a catechol hydrazide, for nanoparti...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9417132/ https://www.ncbi.nlm.nih.gov/pubmed/36133529 http://dx.doi.org/10.1039/c9na00376b |
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author | Fallows, Thomas W. McGrath, Andrew J. Silva, Joana McAdams, Simon G. Marchesi, Andrea Tuna, Floriana Flitsch, Sabine L. Tilley, Richard D. Webb, Simon J. |
author_facet | Fallows, Thomas W. McGrath, Andrew J. Silva, Joana McAdams, Simon G. Marchesi, Andrea Tuna, Floriana Flitsch, Sabine L. Tilley, Richard D. Webb, Simon J. |
author_sort | Fallows, Thomas W. |
collection | PubMed |
description | There is a need for biofunctionalised magnetic nanoparticles for many biomedical applications, including MRI contrast agents that have a range of surface properties and functional groups. A library of eleven adducts, each formed by condensing a reducing sugar with a catechol hydrazide, for nanoparticle functionalisation has been created using a high-throughput chemical synthesis methodology. The enzymatic transformation of an N-acetylglucosamine (GlcNAc) adduct into an N-acetyllactosamine adduct by β-1,4-galactosyltransferase illustrates how chemoenzymatic methods could provide adducts bearing complex and expensive glycans. Superparamagnetic iron oxide nanoparticles (8 nm diameter, characterised by TEM, DLS and SQUID) were coated with these adducts and the magnetic resonance imaging (MRI) properties of GlcNAc-labelled nanoparticles were determined. This straightforward approach can produce a range of MRI contrast agents with a variety of biofunctionalised surfaces. |
format | Online Article Text |
id | pubmed-9417132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | RSC |
record_format | MEDLINE/PubMed |
spelling | pubmed-94171322022-09-20 High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI Fallows, Thomas W. McGrath, Andrew J. Silva, Joana McAdams, Simon G. Marchesi, Andrea Tuna, Floriana Flitsch, Sabine L. Tilley, Richard D. Webb, Simon J. Nanoscale Adv Chemistry There is a need for biofunctionalised magnetic nanoparticles for many biomedical applications, including MRI contrast agents that have a range of surface properties and functional groups. A library of eleven adducts, each formed by condensing a reducing sugar with a catechol hydrazide, for nanoparticle functionalisation has been created using a high-throughput chemical synthesis methodology. The enzymatic transformation of an N-acetylglucosamine (GlcNAc) adduct into an N-acetyllactosamine adduct by β-1,4-galactosyltransferase illustrates how chemoenzymatic methods could provide adducts bearing complex and expensive glycans. Superparamagnetic iron oxide nanoparticles (8 nm diameter, characterised by TEM, DLS and SQUID) were coated with these adducts and the magnetic resonance imaging (MRI) properties of GlcNAc-labelled nanoparticles were determined. This straightforward approach can produce a range of MRI contrast agents with a variety of biofunctionalised surfaces. RSC 2019-07-29 /pmc/articles/PMC9417132/ /pubmed/36133529 http://dx.doi.org/10.1039/c9na00376b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Fallows, Thomas W. McGrath, Andrew J. Silva, Joana McAdams, Simon G. Marchesi, Andrea Tuna, Floriana Flitsch, Sabine L. Tilley, Richard D. Webb, Simon J. High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI |
title | High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI |
title_full | High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI |
title_fullStr | High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI |
title_full_unstemmed | High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI |
title_short | High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI |
title_sort | high-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for mri |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9417132/ https://www.ncbi.nlm.nih.gov/pubmed/36133529 http://dx.doi.org/10.1039/c9na00376b |
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