Purification of quantum dot-based bioprobes with a salting out strategy

A salting out strategy is reported for purification of IgG-conjugated QD (IgG-QD) bioprobes. Adding NaCl can precipitate free IgG selectively, while the IgG-QD maintains good colloidal stability. The dynamic light scattering technique reveals that this is due to the relatively positive zeta potentia...

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Detalles Bibliográficos
Autores principales: Chen, Zhi-Liang, Xu, Jia-quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9417310/
https://www.ncbi.nlm.nih.gov/pubmed/36132697
http://dx.doi.org/10.1039/d1na00569c
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author Chen, Zhi-Liang
Xu, Jia-quan
author_facet Chen, Zhi-Liang
Xu, Jia-quan
author_sort Chen, Zhi-Liang
collection PubMed
description A salting out strategy is reported for purification of IgG-conjugated QD (IgG-QD) bioprobes. Adding NaCl can precipitate free IgG selectively, while the IgG-QD maintains good colloidal stability. The dynamic light scattering technique reveals that this is due to the relatively positive zeta potential of free IgG than that of the IgG-QD.
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spelling pubmed-94173102022-09-20 Purification of quantum dot-based bioprobes with a salting out strategy Chen, Zhi-Liang Xu, Jia-quan Nanoscale Adv Chemistry A salting out strategy is reported for purification of IgG-conjugated QD (IgG-QD) bioprobes. Adding NaCl can precipitate free IgG selectively, while the IgG-QD maintains good colloidal stability. The dynamic light scattering technique reveals that this is due to the relatively positive zeta potential of free IgG than that of the IgG-QD. RSC 2022-01-04 /pmc/articles/PMC9417310/ /pubmed/36132697 http://dx.doi.org/10.1039/d1na00569c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Chen, Zhi-Liang
Xu, Jia-quan
Purification of quantum dot-based bioprobes with a salting out strategy
title Purification of quantum dot-based bioprobes with a salting out strategy
title_full Purification of quantum dot-based bioprobes with a salting out strategy
title_fullStr Purification of quantum dot-based bioprobes with a salting out strategy
title_full_unstemmed Purification of quantum dot-based bioprobes with a salting out strategy
title_short Purification of quantum dot-based bioprobes with a salting out strategy
title_sort purification of quantum dot-based bioprobes with a salting out strategy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9417310/
https://www.ncbi.nlm.nih.gov/pubmed/36132697
http://dx.doi.org/10.1039/d1na00569c
work_keys_str_mv AT chenzhiliang purificationofquantumdotbasedbioprobeswithasaltingoutstrategy
AT xujiaquan purificationofquantumdotbasedbioprobeswithasaltingoutstrategy

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