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Functionalized carbon nanotube electrodes for controlled DNA sequencing
In the last decade, solid-state nanopores/nanogaps have attracted significant attention in the rapid detection of DNA nucleotides. However, reducing the noise through controlled translocation of the DNA nucleobases is a central issue for the development of nanogap/nanopore-based DNA sequencing to ac...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9417824/ https://www.ncbi.nlm.nih.gov/pubmed/36132799 http://dx.doi.org/10.1039/d0na00241k |
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author | Kumawat, Rameshwar L. Pathak, Biswarup |
author_facet | Kumawat, Rameshwar L. Pathak, Biswarup |
author_sort | Kumawat, Rameshwar L. |
collection | PubMed |
description | In the last decade, solid-state nanopores/nanogaps have attracted significant attention in the rapid detection of DNA nucleotides. However, reducing the noise through controlled translocation of the DNA nucleobases is a central issue for the development of nanogap/nanopore-based DNA sequencing to achieve single-nucleobase resolution. Furthermore, the high reactivity of the graphene pores/gaps causes clogging of the pore/gap, leading to the blockage of the pores/gaps, sticking, and irreversible pore closure. To address the prospective of functionalization of the carbon nanostructure and for accomplishing this objective, herein, we have studied the performance of functionalized closed-end cap armchair carbon nanotube (CNT) nanogap-embedded electrodes, which can improve the coupling through non-bonding electrons and may provide the possibility of N/O–H⋯π interactions with the nucleotides, as single-stranded DNA is transmigrated across the electrode. We have investigated the effect of functionalizing the closed-end cap CNT (6,6) electrodes with purine (adenine, guanine) and pyrimidine (thymine, cytosine) molecules. Weak hydrogen bonds formed between the probe molecule and the target DNA nucleobase enhance the electronic coupling and temporarily stabilize the translocating nucleobase against the orientational fluctuations, which may reduce noise in the current signal during experimental measurements. The findings of our density functional theory and non-equilibrium Green's function-based study indicate that this modeled setup could allow DNA nucleotide sequencing with a better and reliable yield, giving current traces that differ by at least 1 order of current magnitude for all the four target nucleotides. Thus, we feel that the functionalized armchair CNT (6,6) nanogap-embedded electrodes may be utilized for controlled DNA sequencing. |
format | Online Article Text |
id | pubmed-9417824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | RSC |
record_format | MEDLINE/PubMed |
spelling | pubmed-94178242022-09-20 Functionalized carbon nanotube electrodes for controlled DNA sequencing Kumawat, Rameshwar L. Pathak, Biswarup Nanoscale Adv Chemistry In the last decade, solid-state nanopores/nanogaps have attracted significant attention in the rapid detection of DNA nucleotides. However, reducing the noise through controlled translocation of the DNA nucleobases is a central issue for the development of nanogap/nanopore-based DNA sequencing to achieve single-nucleobase resolution. Furthermore, the high reactivity of the graphene pores/gaps causes clogging of the pore/gap, leading to the blockage of the pores/gaps, sticking, and irreversible pore closure. To address the prospective of functionalization of the carbon nanostructure and for accomplishing this objective, herein, we have studied the performance of functionalized closed-end cap armchair carbon nanotube (CNT) nanogap-embedded electrodes, which can improve the coupling through non-bonding electrons and may provide the possibility of N/O–H⋯π interactions with the nucleotides, as single-stranded DNA is transmigrated across the electrode. We have investigated the effect of functionalizing the closed-end cap CNT (6,6) electrodes with purine (adenine, guanine) and pyrimidine (thymine, cytosine) molecules. Weak hydrogen bonds formed between the probe molecule and the target DNA nucleobase enhance the electronic coupling and temporarily stabilize the translocating nucleobase against the orientational fluctuations, which may reduce noise in the current signal during experimental measurements. The findings of our density functional theory and non-equilibrium Green's function-based study indicate that this modeled setup could allow DNA nucleotide sequencing with a better and reliable yield, giving current traces that differ by at least 1 order of current magnitude for all the four target nucleotides. Thus, we feel that the functionalized armchair CNT (6,6) nanogap-embedded electrodes may be utilized for controlled DNA sequencing. RSC 2020-07-14 /pmc/articles/PMC9417824/ /pubmed/36132799 http://dx.doi.org/10.1039/d0na00241k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Kumawat, Rameshwar L. Pathak, Biswarup Functionalized carbon nanotube electrodes for controlled DNA sequencing |
title | Functionalized carbon nanotube electrodes for controlled DNA sequencing |
title_full | Functionalized carbon nanotube electrodes for controlled DNA sequencing |
title_fullStr | Functionalized carbon nanotube electrodes for controlled DNA sequencing |
title_full_unstemmed | Functionalized carbon nanotube electrodes for controlled DNA sequencing |
title_short | Functionalized carbon nanotube electrodes for controlled DNA sequencing |
title_sort | functionalized carbon nanotube electrodes for controlled dna sequencing |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9417824/ https://www.ncbi.nlm.nih.gov/pubmed/36132799 http://dx.doi.org/10.1039/d0na00241k |
work_keys_str_mv | AT kumawatrameshwarl functionalizedcarbonnanotubeelectrodesforcontrolleddnasequencing AT pathakbiswarup functionalizedcarbonnanotubeelectrodesforcontrolleddnasequencing |