Cargando…
Bio-electrostatic sensitive droplet lasers for molecular detection
Electrostatics plays a critical function in most biomolecules, therefore monitoring molecular electrostatic interactions at the biointerface can provide the basis in diagnosis and biomedical science. Herein we report a bioelectrostatic responsive microlaser based on liquid crystal (LC) droplets and...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418021/ https://www.ncbi.nlm.nih.gov/pubmed/36132400 http://dx.doi.org/10.1039/d0na00107d |
_version_ | 1784776855435345920 |
---|---|
author | Wang, Ziyihui Zhang, Yifan Gong, Xuerui Yuan, Zhiyi Feng, Shilun Xu, Tianhua Liu, Tiegen Chen, Yu-Cheng |
author_facet | Wang, Ziyihui Zhang, Yifan Gong, Xuerui Yuan, Zhiyi Feng, Shilun Xu, Tianhua Liu, Tiegen Chen, Yu-Cheng |
author_sort | Wang, Ziyihui |
collection | PubMed |
description | Electrostatics plays a critical function in most biomolecules, therefore monitoring molecular electrostatic interactions at the biointerface can provide the basis in diagnosis and biomedical science. Herein we report a bioelectrostatic responsive microlaser based on liquid crystal (LC) droplets and explored its application for the ultrasensitive detection of negatively charged biomolecules. A whispering gallery mode (WGM) laser from positively charged LC microdroplets was designed as the optical resonator, in which the lasing wavelength shift was employed as the sensing parameter. We verified that molecular electrostatic changes at the biointerface of the droplet trigger a wavelength shift in laser spectra. Compared to a conventional polarized optical microscope, a significantly improved sensitivity and dynamic range by four orders of magnitude were achieved. Our results helped discover that the surface-to-volume ratio plays a critical role in the detection sensitivity in WGM laser-based microsensors. Finally, bovine serum albumin and specific biosensing were exploited to demonstrate the potential applications of microlasers with a detection limit in the order of 1 pM, thus offering new alternatives for ultrasensitive label-free biosensing and monitoring of molecular interactions. |
format | Online Article Text |
id | pubmed-9418021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | RSC |
record_format | MEDLINE/PubMed |
spelling | pubmed-94180212022-09-20 Bio-electrostatic sensitive droplet lasers for molecular detection Wang, Ziyihui Zhang, Yifan Gong, Xuerui Yuan, Zhiyi Feng, Shilun Xu, Tianhua Liu, Tiegen Chen, Yu-Cheng Nanoscale Adv Chemistry Electrostatics plays a critical function in most biomolecules, therefore monitoring molecular electrostatic interactions at the biointerface can provide the basis in diagnosis and biomedical science. Herein we report a bioelectrostatic responsive microlaser based on liquid crystal (LC) droplets and explored its application for the ultrasensitive detection of negatively charged biomolecules. A whispering gallery mode (WGM) laser from positively charged LC microdroplets was designed as the optical resonator, in which the lasing wavelength shift was employed as the sensing parameter. We verified that molecular electrostatic changes at the biointerface of the droplet trigger a wavelength shift in laser spectra. Compared to a conventional polarized optical microscope, a significantly improved sensitivity and dynamic range by four orders of magnitude were achieved. Our results helped discover that the surface-to-volume ratio plays a critical role in the detection sensitivity in WGM laser-based microsensors. Finally, bovine serum albumin and specific biosensing were exploited to demonstrate the potential applications of microlasers with a detection limit in the order of 1 pM, thus offering new alternatives for ultrasensitive label-free biosensing and monitoring of molecular interactions. RSC 2020-05-19 /pmc/articles/PMC9418021/ /pubmed/36132400 http://dx.doi.org/10.1039/d0na00107d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Wang, Ziyihui Zhang, Yifan Gong, Xuerui Yuan, Zhiyi Feng, Shilun Xu, Tianhua Liu, Tiegen Chen, Yu-Cheng Bio-electrostatic sensitive droplet lasers for molecular detection |
title | Bio-electrostatic sensitive droplet lasers for molecular detection |
title_full | Bio-electrostatic sensitive droplet lasers for molecular detection |
title_fullStr | Bio-electrostatic sensitive droplet lasers for molecular detection |
title_full_unstemmed | Bio-electrostatic sensitive droplet lasers for molecular detection |
title_short | Bio-electrostatic sensitive droplet lasers for molecular detection |
title_sort | bio-electrostatic sensitive droplet lasers for molecular detection |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418021/ https://www.ncbi.nlm.nih.gov/pubmed/36132400 http://dx.doi.org/10.1039/d0na00107d |
work_keys_str_mv | AT wangziyihui bioelectrostaticsensitivedropletlasersformoleculardetection AT zhangyifan bioelectrostaticsensitivedropletlasersformoleculardetection AT gongxuerui bioelectrostaticsensitivedropletlasersformoleculardetection AT yuanzhiyi bioelectrostaticsensitivedropletlasersformoleculardetection AT fengshilun bioelectrostaticsensitivedropletlasersformoleculardetection AT xutianhua bioelectrostaticsensitivedropletlasersformoleculardetection AT liutiegen bioelectrostaticsensitivedropletlasersformoleculardetection AT chenyucheng bioelectrostaticsensitivedropletlasersformoleculardetection |