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Efficient DNA fluorescence labeling via base excision trapping

Fluorescence labeling of DNAs is broadly useful, but methods for labeling are expensive and labor-intensive. Here we describe a general method for fluorescence labeling of oligonucleotides readily and cost-efficiently via base excision trapping (BETr), employing deaminated DNA bases to mark label po...

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Detalles Bibliográficos
Autores principales: Jun, Yong Woong, Harcourt, Emily M., Xiao, Lu, Wilson, David L., Kool, Eric T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418136/
https://www.ncbi.nlm.nih.gov/pubmed/36028479
http://dx.doi.org/10.1038/s41467-022-32494-8