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Insulin sensitivity is associated with the observed variation of de novo lipid synthesis and body composition in finishing pigs

Variations in body composition among pigs can be associated with insulin sensitivity given the insulin anabolic effect. The study objectives were to characterize this association and to compare de novo lipogenesis and the gene expression in the adipose tissue of pigs of the same genetic background....

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Autores principales: Salgado, Hector Hernando, Pomar, Candido, Palin, Marie-France, Lapierre, Hélène, Létourneau-Montminy, Marie-Pierre, Cant, John P., Remus, Aline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418310/
https://www.ncbi.nlm.nih.gov/pubmed/36028540
http://dx.doi.org/10.1038/s41598-022-18799-0
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author Salgado, Hector Hernando
Pomar, Candido
Palin, Marie-France
Lapierre, Hélène
Létourneau-Montminy, Marie-Pierre
Cant, John P.
Remus, Aline
author_facet Salgado, Hector Hernando
Pomar, Candido
Palin, Marie-France
Lapierre, Hélène
Létourneau-Montminy, Marie-Pierre
Cant, John P.
Remus, Aline
author_sort Salgado, Hector Hernando
collection PubMed
description Variations in body composition among pigs can be associated with insulin sensitivity given the insulin anabolic effect. The study objectives were to characterize this association and to compare de novo lipogenesis and the gene expression in the adipose tissue of pigs of the same genetic background. Thirty 30–95 kg of body weight (BW) pigs, catheterized in the jugular vein participated into an oral glucose tolerance test (OGTT; 1.75 g glucose/kg of BW) to calculate insulin-related indexes. The 8 fattest and the 8 leanest pigs were used to determine the relative mRNA abundance of studied genes. The rate of lipogenesis was assessed by incorporation of [U-(13)C]glucose into lipids. The QUICKI and Matsuda indexes negatively correlated with total body lipids (r =  − 0.67 and r =  − 0.59; P < 0.01) and de novo lipogenesis (r =  − 0.58; P < 0.01). Fat pigs had a higher expression level of lipogenic enzymes (ACACA, ACLY; P < 0.05) than lean pigs. The reduced insulin sensitivity in fat pigs was associated with a higher expression level of glucose-6-phosphate dehydrogenase (G6PD) and a lower expression of peroxisome proliferator-activated receptor-gamma (PPAR-γ). In conclusion, pigs with increased body lipids have lower insulin sensitivity which is associated with increased de novo lipogenesis.
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spelling pubmed-94183102022-08-28 Insulin sensitivity is associated with the observed variation of de novo lipid synthesis and body composition in finishing pigs Salgado, Hector Hernando Pomar, Candido Palin, Marie-France Lapierre, Hélène Létourneau-Montminy, Marie-Pierre Cant, John P. Remus, Aline Sci Rep Article Variations in body composition among pigs can be associated with insulin sensitivity given the insulin anabolic effect. The study objectives were to characterize this association and to compare de novo lipogenesis and the gene expression in the adipose tissue of pigs of the same genetic background. Thirty 30–95 kg of body weight (BW) pigs, catheterized in the jugular vein participated into an oral glucose tolerance test (OGTT; 1.75 g glucose/kg of BW) to calculate insulin-related indexes. The 8 fattest and the 8 leanest pigs were used to determine the relative mRNA abundance of studied genes. The rate of lipogenesis was assessed by incorporation of [U-(13)C]glucose into lipids. The QUICKI and Matsuda indexes negatively correlated with total body lipids (r =  − 0.67 and r =  − 0.59; P < 0.01) and de novo lipogenesis (r =  − 0.58; P < 0.01). Fat pigs had a higher expression level of lipogenic enzymes (ACACA, ACLY; P < 0.05) than lean pigs. The reduced insulin sensitivity in fat pigs was associated with a higher expression level of glucose-6-phosphate dehydrogenase (G6PD) and a lower expression of peroxisome proliferator-activated receptor-gamma (PPAR-γ). In conclusion, pigs with increased body lipids have lower insulin sensitivity which is associated with increased de novo lipogenesis. Nature Publishing Group UK 2022-08-26 /pmc/articles/PMC9418310/ /pubmed/36028540 http://dx.doi.org/10.1038/s41598-022-18799-0 Text en © Crown 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Salgado, Hector Hernando
Pomar, Candido
Palin, Marie-France
Lapierre, Hélène
Létourneau-Montminy, Marie-Pierre
Cant, John P.
Remus, Aline
Insulin sensitivity is associated with the observed variation of de novo lipid synthesis and body composition in finishing pigs
title Insulin sensitivity is associated with the observed variation of de novo lipid synthesis and body composition in finishing pigs
title_full Insulin sensitivity is associated with the observed variation of de novo lipid synthesis and body composition in finishing pigs
title_fullStr Insulin sensitivity is associated with the observed variation of de novo lipid synthesis and body composition in finishing pigs
title_full_unstemmed Insulin sensitivity is associated with the observed variation of de novo lipid synthesis and body composition in finishing pigs
title_short Insulin sensitivity is associated with the observed variation of de novo lipid synthesis and body composition in finishing pigs
title_sort insulin sensitivity is associated with the observed variation of de novo lipid synthesis and body composition in finishing pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418310/
https://www.ncbi.nlm.nih.gov/pubmed/36028540
http://dx.doi.org/10.1038/s41598-022-18799-0
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