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Bio-inspired temporal regulation of ion-transport in nanochannels

Temporal regulation of mass transport across the membrane is a vital feature of biological systems. Such regulatory mechanisms rely on complex biochemical reaction networks, often operating far from equilibrium. Herein, we demonstrate biochemical reaction mediated temporal regulation of mass transpo...

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Detalles Bibliográficos
Autores principales: Sonu, K. P., Vinikumar, Sushmitha, Dhiman, Shikha, George, Subi J., Eswaramoorthy, Muthusamy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418411/
https://www.ncbi.nlm.nih.gov/pubmed/36134245
http://dx.doi.org/10.1039/c8na00414e
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author Sonu, K. P.
Vinikumar, Sushmitha
Dhiman, Shikha
George, Subi J.
Eswaramoorthy, Muthusamy
author_facet Sonu, K. P.
Vinikumar, Sushmitha
Dhiman, Shikha
George, Subi J.
Eswaramoorthy, Muthusamy
author_sort Sonu, K. P.
collection PubMed
description Temporal regulation of mass transport across the membrane is a vital feature of biological systems. Such regulatory mechanisms rely on complex biochemical reaction networks, often operating far from equilibrium. Herein, we demonstrate biochemical reaction mediated temporal regulation of mass transport in nanochannels of mesoporous silica sphere. The rationally designed nanochannels with pH responsive electrostatic gating are fabricated through a hetero-functionalization approach utilizing propylamine and carboxylic acid moieties. At basic pH, cationic small molecules can diffuse into the nanochannels which release back to the solution at acidic pH. The transient ion transport is temporally controlled using a base as fuel along with esterase enzyme as the mediator. The slow enzymatic hydrolysis of a dormant deactivator (ethyl acetate) determines the lifetime of transient encapsulated state, which can be programmed easily by modulating the enzymatic activity of esterase. This system represents a unique approach to create autonomous artificial cellular models.
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spelling pubmed-94184112022-09-20 Bio-inspired temporal regulation of ion-transport in nanochannels Sonu, K. P. Vinikumar, Sushmitha Dhiman, Shikha George, Subi J. Eswaramoorthy, Muthusamy Nanoscale Adv Chemistry Temporal regulation of mass transport across the membrane is a vital feature of biological systems. Such regulatory mechanisms rely on complex biochemical reaction networks, often operating far from equilibrium. Herein, we demonstrate biochemical reaction mediated temporal regulation of mass transport in nanochannels of mesoporous silica sphere. The rationally designed nanochannels with pH responsive electrostatic gating are fabricated through a hetero-functionalization approach utilizing propylamine and carboxylic acid moieties. At basic pH, cationic small molecules can diffuse into the nanochannels which release back to the solution at acidic pH. The transient ion transport is temporally controlled using a base as fuel along with esterase enzyme as the mediator. The slow enzymatic hydrolysis of a dormant deactivator (ethyl acetate) determines the lifetime of transient encapsulated state, which can be programmed easily by modulating the enzymatic activity of esterase. This system represents a unique approach to create autonomous artificial cellular models. RSC 2019-03-12 /pmc/articles/PMC9418411/ /pubmed/36134245 http://dx.doi.org/10.1039/c8na00414e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Sonu, K. P.
Vinikumar, Sushmitha
Dhiman, Shikha
George, Subi J.
Eswaramoorthy, Muthusamy
Bio-inspired temporal regulation of ion-transport in nanochannels
title Bio-inspired temporal regulation of ion-transport in nanochannels
title_full Bio-inspired temporal regulation of ion-transport in nanochannels
title_fullStr Bio-inspired temporal regulation of ion-transport in nanochannels
title_full_unstemmed Bio-inspired temporal regulation of ion-transport in nanochannels
title_short Bio-inspired temporal regulation of ion-transport in nanochannels
title_sort bio-inspired temporal regulation of ion-transport in nanochannels
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418411/
https://www.ncbi.nlm.nih.gov/pubmed/36134245
http://dx.doi.org/10.1039/c8na00414e
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