Isolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium Synechocystis sp. PCC 6803

The FoF1 ATP synthase (ATPase) is one of the most important protein complexes in energy metabolism. The isolation of functional ATPase complexes is fundamental to address questions about its assembly, regulation, and functions. This protocol describes the purification of intact and active ATPase fro...

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Detalles Bibliográficos
Autores principales: Song, Kuo, Tholen, Stefan, Baumgartner, Desirée, Schilling, Oliver, Hess, Wolfgang R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418591/
https://www.ncbi.nlm.nih.gov/pubmed/36039073
http://dx.doi.org/10.1016/j.xpro.2022.101623

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