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The molecular mechanisms underlying mussel adhesion

Marine mussels are able to firmly affix on various wet surfaces by the overproduction of special mussel foot proteins (mfps). Abundant fundamental studies have been conducted to understand the molecular basis of mussel adhesion, where the catecholic amino acid, l-3,4-dihydroxyphenylalanine (DOPA) ha...

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Autores principales: Li, Yiran, Cao, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418609/
https://www.ncbi.nlm.nih.gov/pubmed/36134404
http://dx.doi.org/10.1039/c9na00582j
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author Li, Yiran
Cao, Yi
author_facet Li, Yiran
Cao, Yi
author_sort Li, Yiran
collection PubMed
description Marine mussels are able to firmly affix on various wet surfaces by the overproduction of special mussel foot proteins (mfps). Abundant fundamental studies have been conducted to understand the molecular basis of mussel adhesion, where the catecholic amino acid, l-3,4-dihydroxyphenylalanine (DOPA) has been found to play the major role. These studies continue to inspire the engineering of novel adhesives and coatings with improved underwater performances. Despite the fact that the recent advances of adhesives and coatings inspired by mussel adhesive proteins have been intensively reviewed in literature, the fundamental biochemical and biophysical studies on the origin of the strong and versatile wet adhesion have not been fully covered. In this review, we show how the force measurements at the molecular level by surface force apparatus (SFA) and single molecule atomic force microscopy (AFM) can be used to reveal the direct link between DOPA and the wet adhesion strength of mussel proteins. We highlight a few important technical details that are critical to the successful experimental design. We also summarize many new insights going beyond DOPA adhesion, such as the surface environment and protein sequence dependent synergistic and cooperative binding. We also provide a perspective on a few uncharted but outstanding questions for future studies. A comprehensive understanding on mussel adhesion will be beneficial to the design of novel synthetic wet adhesives for various biomedical applications.
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spelling pubmed-94186092022-09-20 The molecular mechanisms underlying mussel adhesion Li, Yiran Cao, Yi Nanoscale Adv Chemistry Marine mussels are able to firmly affix on various wet surfaces by the overproduction of special mussel foot proteins (mfps). Abundant fundamental studies have been conducted to understand the molecular basis of mussel adhesion, where the catecholic amino acid, l-3,4-dihydroxyphenylalanine (DOPA) has been found to play the major role. These studies continue to inspire the engineering of novel adhesives and coatings with improved underwater performances. Despite the fact that the recent advances of adhesives and coatings inspired by mussel adhesive proteins have been intensively reviewed in literature, the fundamental biochemical and biophysical studies on the origin of the strong and versatile wet adhesion have not been fully covered. In this review, we show how the force measurements at the molecular level by surface force apparatus (SFA) and single molecule atomic force microscopy (AFM) can be used to reveal the direct link between DOPA and the wet adhesion strength of mussel proteins. We highlight a few important technical details that are critical to the successful experimental design. We also summarize many new insights going beyond DOPA adhesion, such as the surface environment and protein sequence dependent synergistic and cooperative binding. We also provide a perspective on a few uncharted but outstanding questions for future studies. A comprehensive understanding on mussel adhesion will be beneficial to the design of novel synthetic wet adhesives for various biomedical applications. RSC 2019-10-10 /pmc/articles/PMC9418609/ /pubmed/36134404 http://dx.doi.org/10.1039/c9na00582j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Li, Yiran
Cao, Yi
The molecular mechanisms underlying mussel adhesion
title The molecular mechanisms underlying mussel adhesion
title_full The molecular mechanisms underlying mussel adhesion
title_fullStr The molecular mechanisms underlying mussel adhesion
title_full_unstemmed The molecular mechanisms underlying mussel adhesion
title_short The molecular mechanisms underlying mussel adhesion
title_sort molecular mechanisms underlying mussel adhesion
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418609/
https://www.ncbi.nlm.nih.gov/pubmed/36134404
http://dx.doi.org/10.1039/c9na00582j
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