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A multiwavelength emission detector for analytical ultracentrifugation
In this study, a new detector for multiwavelength emission analytical ultracentrifugation (MWE-AUC) is presented, which allows measuring size- or composition-dependent fluorescence properties of nanoparticle ensembles. Validation of the new setup is carried out via comparison to a benchtop photolumi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419176/ https://www.ncbi.nlm.nih.gov/pubmed/36134402 http://dx.doi.org/10.1039/c9na00487d |
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author | Wawra, Simon E. Onishchukov, Georgy Maranska, Maria Eigler, Siegfried Walter, Johannes Peukert, Wolfgang |
author_facet | Wawra, Simon E. Onishchukov, Georgy Maranska, Maria Eigler, Siegfried Walter, Johannes Peukert, Wolfgang |
author_sort | Wawra, Simon E. |
collection | PubMed |
description | In this study, a new detector for multiwavelength emission analytical ultracentrifugation (MWE-AUC) is presented, which allows measuring size- or composition-dependent fluorescence properties of nanoparticle ensembles. Validation of the new setup is carried out via comparison to a benchtop photoluminescence spectrometer and the established extinction-based multiwavelength analytical ultracentrifuge (MWL-AUC). The results on fluorescent proteins and silica particles demonstrate that the new device not only correctly reproduces sedimentation and diffusion coefficients of the particles but provides also meaningful fluorescence spectra. As an application example for a sample exhibiting a broad particle size distribution, spectra and size of graphene oxide nanoplatelets are extracted simultaneously. Narrowly distributed CdSe/ZnS quantum dots showing size- and structure-dependent shifts of their fluorescence spectra are analyzed as well. The combination of MWE- and MWL-AUC provides a comprehensive framework for the optical characterization for nanoparticles and macromolecules in terms of their extinction and emission properties. |
format | Online Article Text |
id | pubmed-9419176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | RSC |
record_format | MEDLINE/PubMed |
spelling | pubmed-94191762022-09-20 A multiwavelength emission detector for analytical ultracentrifugation Wawra, Simon E. Onishchukov, Georgy Maranska, Maria Eigler, Siegfried Walter, Johannes Peukert, Wolfgang Nanoscale Adv Chemistry In this study, a new detector for multiwavelength emission analytical ultracentrifugation (MWE-AUC) is presented, which allows measuring size- or composition-dependent fluorescence properties of nanoparticle ensembles. Validation of the new setup is carried out via comparison to a benchtop photoluminescence spectrometer and the established extinction-based multiwavelength analytical ultracentrifuge (MWL-AUC). The results on fluorescent proteins and silica particles demonstrate that the new device not only correctly reproduces sedimentation and diffusion coefficients of the particles but provides also meaningful fluorescence spectra. As an application example for a sample exhibiting a broad particle size distribution, spectra and size of graphene oxide nanoplatelets are extracted simultaneously. Narrowly distributed CdSe/ZnS quantum dots showing size- and structure-dependent shifts of their fluorescence spectra are analyzed as well. The combination of MWE- and MWL-AUC provides a comprehensive framework for the optical characterization for nanoparticles and macromolecules in terms of their extinction and emission properties. RSC 2019-10-04 /pmc/articles/PMC9419176/ /pubmed/36134402 http://dx.doi.org/10.1039/c9na00487d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Wawra, Simon E. Onishchukov, Georgy Maranska, Maria Eigler, Siegfried Walter, Johannes Peukert, Wolfgang A multiwavelength emission detector for analytical ultracentrifugation |
title | A multiwavelength emission detector for analytical ultracentrifugation |
title_full | A multiwavelength emission detector for analytical ultracentrifugation |
title_fullStr | A multiwavelength emission detector for analytical ultracentrifugation |
title_full_unstemmed | A multiwavelength emission detector for analytical ultracentrifugation |
title_short | A multiwavelength emission detector for analytical ultracentrifugation |
title_sort | multiwavelength emission detector for analytical ultracentrifugation |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419176/ https://www.ncbi.nlm.nih.gov/pubmed/36134402 http://dx.doi.org/10.1039/c9na00487d |
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