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Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach
Detection and size estimation of combustion-derived carbonaceous particles (CDCPs) are important to understand their toxicity. Size determination of individual nano- and microparticles (NMPs) based on scattered light is a straightforward method. However, detection and sizing of CDCPs in biological s...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419628/ https://www.ncbi.nlm.nih.gov/pubmed/36132818 http://dx.doi.org/10.1039/d2na00262k |
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author | Aslam, Imran Fron, Eduard Roeffaers, Maarten B. J. |
author_facet | Aslam, Imran Fron, Eduard Roeffaers, Maarten B. J. |
author_sort | Aslam, Imran |
collection | PubMed |
description | Detection and size estimation of combustion-derived carbonaceous particles (CDCPs) are important to understand their toxicity. Size determination of individual nano- and microparticles (NMPs) based on scattered light is a straightforward method. However, detection and sizing of CDCPs in biological samples based on scattering alone are not possible due to the compositional heterogeneity of NMPs present in biological samples. Label-free identification of CDCPs based on unique white light (WL) emission, using femtosecond (fs) pulsed near-infrared (NIR) lasers, has emerged as a reliable method even in complex biological samples. However, size estimation of CDCPs in biological samples using label-free techniques is still lacking. Here we report the development of a dual-channel multiphoton flow cytometry (DCMPFC) setup for label-free identification and size-determination of CDCPs in suspensions. Scattering intensity calibration with reference polystyrene (PS) nanoparticles (NPs) and Mie Theory allow us to determine the sizes of CDCPs in aqueous suspensions. Further, the relationship between particle sizes and WL emission intensity was determined, and the sizes of CDCPs in urine samples could also be estimated. This approach is believed to open new opportunities for the quantification and size determination of CDCPs, originating from exposure to air pollution, in liquid biopsies. This is an important step in determining the CDCP exposure of individual persons. |
format | Online Article Text |
id | pubmed-9419628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | RSC |
record_format | MEDLINE/PubMed |
spelling | pubmed-94196282022-09-20 Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach Aslam, Imran Fron, Eduard Roeffaers, Maarten B. J. Nanoscale Adv Chemistry Detection and size estimation of combustion-derived carbonaceous particles (CDCPs) are important to understand their toxicity. Size determination of individual nano- and microparticles (NMPs) based on scattered light is a straightforward method. However, detection and sizing of CDCPs in biological samples based on scattering alone are not possible due to the compositional heterogeneity of NMPs present in biological samples. Label-free identification of CDCPs based on unique white light (WL) emission, using femtosecond (fs) pulsed near-infrared (NIR) lasers, has emerged as a reliable method even in complex biological samples. However, size estimation of CDCPs in biological samples using label-free techniques is still lacking. Here we report the development of a dual-channel multiphoton flow cytometry (DCMPFC) setup for label-free identification and size-determination of CDCPs in suspensions. Scattering intensity calibration with reference polystyrene (PS) nanoparticles (NPs) and Mie Theory allow us to determine the sizes of CDCPs in aqueous suspensions. Further, the relationship between particle sizes and WL emission intensity was determined, and the sizes of CDCPs in urine samples could also be estimated. This approach is believed to open new opportunities for the quantification and size determination of CDCPs, originating from exposure to air pollution, in liquid biopsies. This is an important step in determining the CDCP exposure of individual persons. RSC 2022-06-21 /pmc/articles/PMC9419628/ /pubmed/36132818 http://dx.doi.org/10.1039/d2na00262k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Aslam, Imran Fron, Eduard Roeffaers, Maarten B. J. Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach |
title | Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach |
title_full | Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach |
title_fullStr | Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach |
title_full_unstemmed | Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach |
title_short | Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach |
title_sort | label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419628/ https://www.ncbi.nlm.nih.gov/pubmed/36132818 http://dx.doi.org/10.1039/d2na00262k |
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