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Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles
The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using lifetime fingerprints from luminescent nanoparticles. However, the optical diffraction limit compromises the decoding accuracy and throughput of the nanoparticles during conventional widefield im...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419758/ https://www.ncbi.nlm.nih.gov/pubmed/36132948 http://dx.doi.org/10.1039/d1na00765c |
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author | Liu, Baolei Liao, Jiayan Song, Yiliao Chen, Chaohao Ding, Lei Lu, Jie Zhou, Jiajia Wang, Fan |
author_facet | Liu, Baolei Liao, Jiayan Song, Yiliao Chen, Chaohao Ding, Lei Lu, Jie Zhou, Jiajia Wang, Fan |
author_sort | Liu, Baolei |
collection | PubMed |
description | The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using lifetime fingerprints from luminescent nanoparticles. However, the optical diffraction limit compromises the decoding accuracy and throughput of the nanoparticles during conventional widefield imaging. This, in turn, challenges the quality of nanoparticles to afford the modulated excitation condition and further retain the multiplexed optical fingerprints for super-resolution multiplexing. Here we report a tailor-made multiplexed super-resolution imaging method using the lifetime-engineered upconversion nanoparticles. We demonstrate that the nanoparticles are bright, uniform, and stable under structured illumination, which supports a lateral resolution of 185 nm, less than 1/4th of the excitation wavelength. We further develop a deep learning algorithm to coordinate with super-resolution images for more accurate decoding compared to a numeric algorithm. We demonstrate a three-channel super-resolution imaging based optical multiplexing with decoding accuracies above 93% for each channel and larger than 60% accuracy for potential seven-channel multiplexing. The improved resolution provides high throughput by resolving the particles within the diffraction-limited spots, which enables higher multiplexing capacity in space. This lifetime multiplexing super-resolution method opens a new horizon for handling the growing amount of information content, disease source, and security risk in modern society. |
format | Online Article Text |
id | pubmed-9419758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | RSC |
record_format | MEDLINE/PubMed |
spelling | pubmed-94197582022-09-20 Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles Liu, Baolei Liao, Jiayan Song, Yiliao Chen, Chaohao Ding, Lei Lu, Jie Zhou, Jiajia Wang, Fan Nanoscale Adv Chemistry The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using lifetime fingerprints from luminescent nanoparticles. However, the optical diffraction limit compromises the decoding accuracy and throughput of the nanoparticles during conventional widefield imaging. This, in turn, challenges the quality of nanoparticles to afford the modulated excitation condition and further retain the multiplexed optical fingerprints for super-resolution multiplexing. Here we report a tailor-made multiplexed super-resolution imaging method using the lifetime-engineered upconversion nanoparticles. We demonstrate that the nanoparticles are bright, uniform, and stable under structured illumination, which supports a lateral resolution of 185 nm, less than 1/4th of the excitation wavelength. We further develop a deep learning algorithm to coordinate with super-resolution images for more accurate decoding compared to a numeric algorithm. We demonstrate a three-channel super-resolution imaging based optical multiplexing with decoding accuracies above 93% for each channel and larger than 60% accuracy for potential seven-channel multiplexing. The improved resolution provides high throughput by resolving the particles within the diffraction-limited spots, which enables higher multiplexing capacity in space. This lifetime multiplexing super-resolution method opens a new horizon for handling the growing amount of information content, disease source, and security risk in modern society. RSC 2021-11-02 /pmc/articles/PMC9419758/ /pubmed/36132948 http://dx.doi.org/10.1039/d1na00765c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Liu, Baolei Liao, Jiayan Song, Yiliao Chen, Chaohao Ding, Lei Lu, Jie Zhou, Jiajia Wang, Fan Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles |
title | Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles |
title_full | Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles |
title_fullStr | Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles |
title_full_unstemmed | Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles |
title_short | Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles |
title_sort | multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419758/ https://www.ncbi.nlm.nih.gov/pubmed/36132948 http://dx.doi.org/10.1039/d1na00765c |
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