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Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles

The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using lifetime fingerprints from luminescent nanoparticles. However, the optical diffraction limit compromises the decoding accuracy and throughput of the nanoparticles during conventional widefield im...

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Detalles Bibliográficos
Autores principales: Liu, Baolei, Liao, Jiayan, Song, Yiliao, Chen, Chaohao, Ding, Lei, Lu, Jie, Zhou, Jiajia, Wang, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419758/
https://www.ncbi.nlm.nih.gov/pubmed/36132948
http://dx.doi.org/10.1039/d1na00765c
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author Liu, Baolei
Liao, Jiayan
Song, Yiliao
Chen, Chaohao
Ding, Lei
Lu, Jie
Zhou, Jiajia
Wang, Fan
author_facet Liu, Baolei
Liao, Jiayan
Song, Yiliao
Chen, Chaohao
Ding, Lei
Lu, Jie
Zhou, Jiajia
Wang, Fan
author_sort Liu, Baolei
collection PubMed
description The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using lifetime fingerprints from luminescent nanoparticles. However, the optical diffraction limit compromises the decoding accuracy and throughput of the nanoparticles during conventional widefield imaging. This, in turn, challenges the quality of nanoparticles to afford the modulated excitation condition and further retain the multiplexed optical fingerprints for super-resolution multiplexing. Here we report a tailor-made multiplexed super-resolution imaging method using the lifetime-engineered upconversion nanoparticles. We demonstrate that the nanoparticles are bright, uniform, and stable under structured illumination, which supports a lateral resolution of 185 nm, less than 1/4th of the excitation wavelength. We further develop a deep learning algorithm to coordinate with super-resolution images for more accurate decoding compared to a numeric algorithm. We demonstrate a three-channel super-resolution imaging based optical multiplexing with decoding accuracies above 93% for each channel and larger than 60% accuracy for potential seven-channel multiplexing. The improved resolution provides high throughput by resolving the particles within the diffraction-limited spots, which enables higher multiplexing capacity in space. This lifetime multiplexing super-resolution method opens a new horizon for handling the growing amount of information content, disease source, and security risk in modern society.
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spelling pubmed-94197582022-09-20 Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles Liu, Baolei Liao, Jiayan Song, Yiliao Chen, Chaohao Ding, Lei Lu, Jie Zhou, Jiajia Wang, Fan Nanoscale Adv Chemistry The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using lifetime fingerprints from luminescent nanoparticles. However, the optical diffraction limit compromises the decoding accuracy and throughput of the nanoparticles during conventional widefield imaging. This, in turn, challenges the quality of nanoparticles to afford the modulated excitation condition and further retain the multiplexed optical fingerprints for super-resolution multiplexing. Here we report a tailor-made multiplexed super-resolution imaging method using the lifetime-engineered upconversion nanoparticles. We demonstrate that the nanoparticles are bright, uniform, and stable under structured illumination, which supports a lateral resolution of 185 nm, less than 1/4th of the excitation wavelength. We further develop a deep learning algorithm to coordinate with super-resolution images for more accurate decoding compared to a numeric algorithm. We demonstrate a three-channel super-resolution imaging based optical multiplexing with decoding accuracies above 93% for each channel and larger than 60% accuracy for potential seven-channel multiplexing. The improved resolution provides high throughput by resolving the particles within the diffraction-limited spots, which enables higher multiplexing capacity in space. This lifetime multiplexing super-resolution method opens a new horizon for handling the growing amount of information content, disease source, and security risk in modern society. RSC 2021-11-02 /pmc/articles/PMC9419758/ /pubmed/36132948 http://dx.doi.org/10.1039/d1na00765c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Liu, Baolei
Liao, Jiayan
Song, Yiliao
Chen, Chaohao
Ding, Lei
Lu, Jie
Zhou, Jiajia
Wang, Fan
Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles
title Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles
title_full Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles
title_fullStr Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles
title_full_unstemmed Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles
title_short Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles
title_sort multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419758/
https://www.ncbi.nlm.nih.gov/pubmed/36132948
http://dx.doi.org/10.1039/d1na00765c
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