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Establishment of Reference Intervals for Serum Protein Electrophoresis of Apparently Healthy Adults in Addis Ababa, Ethiopia

BACKGROUND: Even though several factors affect reference intervals (RIs), company-derived values are currently in use in many laboratories worldwide. However, few or no data are available regarding serum proteins RIs, especially in resource-limited countries such as Ethiopia. OBJECTIVE: To establish...

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Autores principales: Bikila, Demiraw, Lejisa, Tadesse, Tolcha, Yosef, Bashea, Chala, Meles, Mehari, Getahun, Tigist, Ashebir, Genet, Habtu, Wossene, Challa, Feyissa, Mohammed, Ousman, Kassaw, Melkitu, Kebede, Adisu, G/Egzeabher, Letebrhan, Befekadu, Endalkachew, Wolde, Mistire, Tsegaye, Aster
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419900/
https://www.ncbi.nlm.nih.gov/pubmed/36039308
http://dx.doi.org/10.2147/IJGM.S376450
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author Bikila, Demiraw
Lejisa, Tadesse
Tolcha, Yosef
Bashea, Chala
Meles, Mehari
Getahun, Tigist
Ashebir, Genet
Habtu, Wossene
Challa, Feyissa
Mohammed, Ousman
Kassaw, Melkitu
Kebede, Adisu
G/Egzeabher, Letebrhan
Befekadu, Endalkachew
Wolde, Mistire
Tsegaye, Aster
author_facet Bikila, Demiraw
Lejisa, Tadesse
Tolcha, Yosef
Bashea, Chala
Meles, Mehari
Getahun, Tigist
Ashebir, Genet
Habtu, Wossene
Challa, Feyissa
Mohammed, Ousman
Kassaw, Melkitu
Kebede, Adisu
G/Egzeabher, Letebrhan
Befekadu, Endalkachew
Wolde, Mistire
Tsegaye, Aster
author_sort Bikila, Demiraw
collection PubMed
description BACKGROUND: Even though several factors affect reference intervals (RIs), company-derived values are currently in use in many laboratories worldwide. However, few or no data are available regarding serum proteins RIs, especially in resource-limited countries such as Ethiopia. OBJECTIVE: To establish RIs for serum protein electrophoresis of apparently healthy adults in Addis Ababa, Ethiopia. METHODS: A cross-sectional study was conducted on a total of 297 apparently healthy adults from April to October 2019 in four selected sub-cities (Akaki, Kirkos, Arada, and Yeka) of Addis Ababa, Ethiopia. Laboratory analysis of collected samples was performed using the Capillarys 2 Flex Piercing analyzer, while statistical analysis was conducted using SPSS version 23 and Med-Calc software. The Mann–Whitney test was used to check partitions. A non-parametric method of reference range establishment was performed as per CLSI guideline EP28A3C. RESULTS: The established RIs were: albumin 53.83–64.59%, 52.24–63.55%; alpha-1 globulin 3.04–5.40%, 3.44–5.60%; alpha-2 globulin 8.0–12.67%, 8.44–12.87%; and beta-1 globulin 5.01–7.38%, 5.14–7.86%. Moreover, the albumin to globulin ratios were 1.16–1.8 and 1.09–1.74 for males and females, respectively. The combined RIs for beta-2 globulin and gamma globulin were 2.54–4.90% and 12.40–21.66%, respectively. CONCLUSION: The established reference interval for serum protein fractions revealed gender-specific differences, except for beta-2 globulin and gamma globulin.
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spelling pubmed-94199002022-08-28 Establishment of Reference Intervals for Serum Protein Electrophoresis of Apparently Healthy Adults in Addis Ababa, Ethiopia Bikila, Demiraw Lejisa, Tadesse Tolcha, Yosef Bashea, Chala Meles, Mehari Getahun, Tigist Ashebir, Genet Habtu, Wossene Challa, Feyissa Mohammed, Ousman Kassaw, Melkitu Kebede, Adisu G/Egzeabher, Letebrhan Befekadu, Endalkachew Wolde, Mistire Tsegaye, Aster Int J Gen Med Original Research BACKGROUND: Even though several factors affect reference intervals (RIs), company-derived values are currently in use in many laboratories worldwide. However, few or no data are available regarding serum proteins RIs, especially in resource-limited countries such as Ethiopia. OBJECTIVE: To establish RIs for serum protein electrophoresis of apparently healthy adults in Addis Ababa, Ethiopia. METHODS: A cross-sectional study was conducted on a total of 297 apparently healthy adults from April to October 2019 in four selected sub-cities (Akaki, Kirkos, Arada, and Yeka) of Addis Ababa, Ethiopia. Laboratory analysis of collected samples was performed using the Capillarys 2 Flex Piercing analyzer, while statistical analysis was conducted using SPSS version 23 and Med-Calc software. The Mann–Whitney test was used to check partitions. A non-parametric method of reference range establishment was performed as per CLSI guideline EP28A3C. RESULTS: The established RIs were: albumin 53.83–64.59%, 52.24–63.55%; alpha-1 globulin 3.04–5.40%, 3.44–5.60%; alpha-2 globulin 8.0–12.67%, 8.44–12.87%; and beta-1 globulin 5.01–7.38%, 5.14–7.86%. Moreover, the albumin to globulin ratios were 1.16–1.8 and 1.09–1.74 for males and females, respectively. The combined RIs for beta-2 globulin and gamma globulin were 2.54–4.90% and 12.40–21.66%, respectively. CONCLUSION: The established reference interval for serum protein fractions revealed gender-specific differences, except for beta-2 globulin and gamma globulin. Dove 2022-08-23 /pmc/articles/PMC9419900/ /pubmed/36039308 http://dx.doi.org/10.2147/IJGM.S376450 Text en © 2022 Bikila et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Bikila, Demiraw
Lejisa, Tadesse
Tolcha, Yosef
Bashea, Chala
Meles, Mehari
Getahun, Tigist
Ashebir, Genet
Habtu, Wossene
Challa, Feyissa
Mohammed, Ousman
Kassaw, Melkitu
Kebede, Adisu
G/Egzeabher, Letebrhan
Befekadu, Endalkachew
Wolde, Mistire
Tsegaye, Aster
Establishment of Reference Intervals for Serum Protein Electrophoresis of Apparently Healthy Adults in Addis Ababa, Ethiopia
title Establishment of Reference Intervals for Serum Protein Electrophoresis of Apparently Healthy Adults in Addis Ababa, Ethiopia
title_full Establishment of Reference Intervals for Serum Protein Electrophoresis of Apparently Healthy Adults in Addis Ababa, Ethiopia
title_fullStr Establishment of Reference Intervals for Serum Protein Electrophoresis of Apparently Healthy Adults in Addis Ababa, Ethiopia
title_full_unstemmed Establishment of Reference Intervals for Serum Protein Electrophoresis of Apparently Healthy Adults in Addis Ababa, Ethiopia
title_short Establishment of Reference Intervals for Serum Protein Electrophoresis of Apparently Healthy Adults in Addis Ababa, Ethiopia
title_sort establishment of reference intervals for serum protein electrophoresis of apparently healthy adults in addis ababa, ethiopia
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419900/
https://www.ncbi.nlm.nih.gov/pubmed/36039308
http://dx.doi.org/10.2147/IJGM.S376450
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