Detection of m(6)A in single cultured cells using scDART-seq

Most techniques for mapping m(6)A-methylated RNAs transcriptome-wide require large amounts of RNA and have been limited to bulk cells and tissues. Here, we provide a detailed protocol for the identification of m(6)A sites in single HEK293T cells using single-cell DART-seq (scDART-seq). The protocol...

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Detalles Bibliográficos
Autores principales: Tegowski, Matthew, Meyer, Kate D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420395/
https://www.ncbi.nlm.nih.gov/pubmed/36042888
http://dx.doi.org/10.1016/j.xpro.2022.101646

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420395/
https://www.ncbi.nlm.nih.gov/pubmed/36042888
http://dx.doi.org/10.1016/j.xpro.2022.101646

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