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Detection of m(6)A in single cultured cells using scDART-seq
Most techniques for mapping m(6)A-methylated RNAs transcriptome-wide require large amounts of RNA and have been limited to bulk cells and tissues. Here, we provide a detailed protocol for the identification of m(6)A sites in single HEK293T cells using single-cell DART-seq (scDART-seq). The protocol...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420395/ https://www.ncbi.nlm.nih.gov/pubmed/36042888 http://dx.doi.org/10.1016/j.xpro.2022.101646 |