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Dissociation and reaggregation of Hydra vulgaris for studies of self-organization
The remarkable regenerative abilities of the small cnidarian Hydra vulgaris include the capacity to reassemble itself after dissociation into individual cells. Here, we present a robust protocol for the dissociation and reaggregation of Hydra tissue that addresses many common challenges encountered...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420532/ https://www.ncbi.nlm.nih.gov/pubmed/36042889 http://dx.doi.org/10.1016/j.xpro.2022.101504 |
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author | Lovas, Jonathan R. Yuste, Rafael |
author_facet | Lovas, Jonathan R. Yuste, Rafael |
author_sort | Lovas, Jonathan R. |
collection | PubMed |
description | The remarkable regenerative abilities of the small cnidarian Hydra vulgaris include the capacity to reassemble itself after dissociation into individual cells. Here, we present a robust protocol for the dissociation and reaggregation of Hydra tissue that addresses many common challenges encountered during the preparation and execution of the dissociation, as well as the formation and care of regenerating cellular aggregates. Analysis of the process provides insight into the mechanisms of nervous system self-organization. For complete details on the use and execution of this protocol, please refer to Lovas and Yuste (2021). |
format | Online Article Text |
id | pubmed-9420532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-94205322022-08-29 Dissociation and reaggregation of Hydra vulgaris for studies of self-organization Lovas, Jonathan R. Yuste, Rafael STAR Protoc Protocol The remarkable regenerative abilities of the small cnidarian Hydra vulgaris include the capacity to reassemble itself after dissociation into individual cells. Here, we present a robust protocol for the dissociation and reaggregation of Hydra tissue that addresses many common challenges encountered during the preparation and execution of the dissociation, as well as the formation and care of regenerating cellular aggregates. Analysis of the process provides insight into the mechanisms of nervous system self-organization. For complete details on the use and execution of this protocol, please refer to Lovas and Yuste (2021). Elsevier 2022-08-18 /pmc/articles/PMC9420532/ /pubmed/36042889 http://dx.doi.org/10.1016/j.xpro.2022.101504 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Lovas, Jonathan R. Yuste, Rafael Dissociation and reaggregation of Hydra vulgaris for studies of self-organization |
title | Dissociation and reaggregation of Hydra vulgaris for studies of self-organization |
title_full | Dissociation and reaggregation of Hydra vulgaris for studies of self-organization |
title_fullStr | Dissociation and reaggregation of Hydra vulgaris for studies of self-organization |
title_full_unstemmed | Dissociation and reaggregation of Hydra vulgaris for studies of self-organization |
title_short | Dissociation and reaggregation of Hydra vulgaris for studies of self-organization |
title_sort | dissociation and reaggregation of hydra vulgaris for studies of self-organization |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420532/ https://www.ncbi.nlm.nih.gov/pubmed/36042889 http://dx.doi.org/10.1016/j.xpro.2022.101504 |
work_keys_str_mv | AT lovasjonathanr dissociationandreaggregationofhydravulgarisforstudiesofselforganization AT yusterafael dissociationandreaggregationofhydravulgarisforstudiesofselforganization |