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Dissociation and reaggregation of Hydra vulgaris for studies of self-organization

The remarkable regenerative abilities of the small cnidarian Hydra vulgaris include the capacity to reassemble itself after dissociation into individual cells. Here, we present a robust protocol for the dissociation and reaggregation of Hydra tissue that addresses many common challenges encountered...

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Detalles Bibliográficos
Autores principales: Lovas, Jonathan R., Yuste, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420532/
https://www.ncbi.nlm.nih.gov/pubmed/36042889
http://dx.doi.org/10.1016/j.xpro.2022.101504
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author Lovas, Jonathan R.
Yuste, Rafael
author_facet Lovas, Jonathan R.
Yuste, Rafael
author_sort Lovas, Jonathan R.
collection PubMed
description The remarkable regenerative abilities of the small cnidarian Hydra vulgaris include the capacity to reassemble itself after dissociation into individual cells. Here, we present a robust protocol for the dissociation and reaggregation of Hydra tissue that addresses many common challenges encountered during the preparation and execution of the dissociation, as well as the formation and care of regenerating cellular aggregates. Analysis of the process provides insight into the mechanisms of nervous system self-organization. For complete details on the use and execution of this protocol, please refer to Lovas and Yuste (2021).
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spelling pubmed-94205322022-08-29 Dissociation and reaggregation of Hydra vulgaris for studies of self-organization Lovas, Jonathan R. Yuste, Rafael STAR Protoc Protocol The remarkable regenerative abilities of the small cnidarian Hydra vulgaris include the capacity to reassemble itself after dissociation into individual cells. Here, we present a robust protocol for the dissociation and reaggregation of Hydra tissue that addresses many common challenges encountered during the preparation and execution of the dissociation, as well as the formation and care of regenerating cellular aggregates. Analysis of the process provides insight into the mechanisms of nervous system self-organization. For complete details on the use and execution of this protocol, please refer to Lovas and Yuste (2021). Elsevier 2022-08-18 /pmc/articles/PMC9420532/ /pubmed/36042889 http://dx.doi.org/10.1016/j.xpro.2022.101504 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lovas, Jonathan R.
Yuste, Rafael
Dissociation and reaggregation of Hydra vulgaris for studies of self-organization
title Dissociation and reaggregation of Hydra vulgaris for studies of self-organization
title_full Dissociation and reaggregation of Hydra vulgaris for studies of self-organization
title_fullStr Dissociation and reaggregation of Hydra vulgaris for studies of self-organization
title_full_unstemmed Dissociation and reaggregation of Hydra vulgaris for studies of self-organization
title_short Dissociation and reaggregation of Hydra vulgaris for studies of self-organization
title_sort dissociation and reaggregation of hydra vulgaris for studies of self-organization
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420532/
https://www.ncbi.nlm.nih.gov/pubmed/36042889
http://dx.doi.org/10.1016/j.xpro.2022.101504
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