Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells

Human trophoblast stem cells (hTSCs) are useful for studying human placenta development and diseases, but primed human pluripotent stem cells (hPSCs) routinely cultured in most laboratories do not support hTSC derivation. Here, we present a protocol to derive hTSCs directly from primed hPSCs. This a...

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Detalles Bibliográficos
Autores principales: Wei, Yanxing, Xiao, Lu, Ma, Lishi, Wang, Zhijian, Huang, Liping, Li, Huiying, Pan, Guangjin, Lye, Stephen J., Shan, Yongli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420535/
https://www.ncbi.nlm.nih.gov/pubmed/36042882
http://dx.doi.org/10.1016/j.xpro.2022.101638
Descripción
Sumario:Human trophoblast stem cells (hTSCs) are useful for studying human placenta development and diseases, but primed human pluripotent stem cells (hPSCs) routinely cultured in most laboratories do not support hTSC derivation. Here, we present a protocol to derive hTSCs directly from primed hPSCs. This approach, containing two strategies either with or without bone morphogenetic protein 4 (BMP4), provides a simple and accessible tool for deriving hTSCs to study placenta development and disease modeling without ethical limitations or reprogramming process. For complete details on the use and execution of this protocol, please refer to Wei et al. (2021).

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