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Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease

Prior studies reported inconsistent results on the altered gut microbial composition in patients with Crohn’s disease (CD), likely under the influences of many confounding factors including genetic, life style and environmental variations among different study cohorts. This study aims to examine the...

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Autores principales: Hu, Jun, Cheng, Sijing, Yao, Jiayin, Lin, Xutao, Li, Yichen, Wang, Wenxia, Weng, Jingrong, Zou, Yifeng, Zhu, Lixin, Zhi, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420857/
https://www.ncbi.nlm.nih.gov/pubmed/36045690
http://dx.doi.org/10.3389/fimmu.2022.947313
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author Hu, Jun
Cheng, Sijing
Yao, Jiayin
Lin, Xutao
Li, Yichen
Wang, Wenxia
Weng, Jingrong
Zou, Yifeng
Zhu, Lixin
Zhi, Min
author_facet Hu, Jun
Cheng, Sijing
Yao, Jiayin
Lin, Xutao
Li, Yichen
Wang, Wenxia
Weng, Jingrong
Zou, Yifeng
Zhu, Lixin
Zhi, Min
author_sort Hu, Jun
collection PubMed
description Prior studies reported inconsistent results on the altered gut microbial composition in patients with Crohn’s disease (CD), likely under the influences of many confounding factors including genetic, life style and environmental variations among different study cohorts. This study aims to examine the gut microbiota of CD patients with particular efforts to minimize the impact of the confounding factors. For this purpose, the healthy relatives of the patients were enrolled as control subjects so that the paired study subjects may have similar genetic background, dietary habits, and household environment. The fecal microbiota of the study subjects were examined by 16S rRNA sequencing. After the identification of the differential bacterial genera, multivariate regression analysis was performed to adjust the results for the impact of confounding factors. We found that the microbiota of the CD patients were featured with reduced short chain fatty acid (SCFA) producing bacteria and elevated opportunistic pathogen Escherichia-Shigella. Correlation analysis indicated that the elevation in Escherichia-Shigella and the reduction in SCFA-producing bacteria usually occur simultaneously. These differential genera exhibited a high capacity in distinguishing between CD and healthy controls achieving an area under curve of 0.89, and were correlated with the changes in inflammation related blood biochemical markers. Consistent with the reduction in SCFA-producing bacteria in CD, metabolomics analysis revealed decreased blood level of SCFAs in the patients. The differential genera identified in this study demonstrated outstanding capability to serve as diagnosis markers for CD and are potential targets for intervention.
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spelling pubmed-94208572022-08-30 Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease Hu, Jun Cheng, Sijing Yao, Jiayin Lin, Xutao Li, Yichen Wang, Wenxia Weng, Jingrong Zou, Yifeng Zhu, Lixin Zhi, Min Front Immunol Immunology Prior studies reported inconsistent results on the altered gut microbial composition in patients with Crohn’s disease (CD), likely under the influences of many confounding factors including genetic, life style and environmental variations among different study cohorts. This study aims to examine the gut microbiota of CD patients with particular efforts to minimize the impact of the confounding factors. For this purpose, the healthy relatives of the patients were enrolled as control subjects so that the paired study subjects may have similar genetic background, dietary habits, and household environment. The fecal microbiota of the study subjects were examined by 16S rRNA sequencing. After the identification of the differential bacterial genera, multivariate regression analysis was performed to adjust the results for the impact of confounding factors. We found that the microbiota of the CD patients were featured with reduced short chain fatty acid (SCFA) producing bacteria and elevated opportunistic pathogen Escherichia-Shigella. Correlation analysis indicated that the elevation in Escherichia-Shigella and the reduction in SCFA-producing bacteria usually occur simultaneously. These differential genera exhibited a high capacity in distinguishing between CD and healthy controls achieving an area under curve of 0.89, and were correlated with the changes in inflammation related blood biochemical markers. Consistent with the reduction in SCFA-producing bacteria in CD, metabolomics analysis revealed decreased blood level of SCFAs in the patients. The differential genera identified in this study demonstrated outstanding capability to serve as diagnosis markers for CD and are potential targets for intervention. Frontiers Media S.A. 2022-08-15 /pmc/articles/PMC9420857/ /pubmed/36045690 http://dx.doi.org/10.3389/fimmu.2022.947313 Text en Copyright © 2022 Hu, Cheng, Yao, Lin, Li, Wang, Weng, Zou, Zhu and Zhi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Hu, Jun
Cheng, Sijing
Yao, Jiayin
Lin, Xutao
Li, Yichen
Wang, Wenxia
Weng, Jingrong
Zou, Yifeng
Zhu, Lixin
Zhi, Min
Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease
title Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease
title_full Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease
title_fullStr Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease
title_full_unstemmed Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease
title_short Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease
title_sort correlation between altered gut microbiota and elevated inflammation markers in patients with crohn’s disease
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420857/
https://www.ncbi.nlm.nih.gov/pubmed/36045690
http://dx.doi.org/10.3389/fimmu.2022.947313
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