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The survivor strain: isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential

Despite their recognized potential, current applications of cyanobacteria as microbial cell factories remain in early stages of development. This is partly due to the fact that engineered strains are often difficult to grow at scale. This technical challenge contrasts with the dense and highly produ...

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Autores principales: Koch, Moritz, Noonan, Avery J. C., Qiu, Yilin, Dofher, Kalen, Kieft, Brandon, Mottahedeh, Soheyl, Shastri, Manisha, Hallam, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420970/
https://www.ncbi.nlm.nih.gov/pubmed/36046667
http://dx.doi.org/10.3389/fbioe.2022.932695
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author Koch, Moritz
Noonan, Avery J. C.
Qiu, Yilin
Dofher, Kalen
Kieft, Brandon
Mottahedeh, Soheyl
Shastri, Manisha
Hallam, Steven J.
author_facet Koch, Moritz
Noonan, Avery J. C.
Qiu, Yilin
Dofher, Kalen
Kieft, Brandon
Mottahedeh, Soheyl
Shastri, Manisha
Hallam, Steven J.
author_sort Koch, Moritz
collection PubMed
description Despite their recognized potential, current applications of cyanobacteria as microbial cell factories remain in early stages of development. This is partly due to the fact that engineered strains are often difficult to grow at scale. This technical challenge contrasts with the dense and highly productive cyanobacteria populations thriving in many natural environments. It has been proposed that the selection of strains pre-adapted for growth in industrial photobioreactors could enable more productive cultivation outcomes. Here, we described the initial morphological, physiological, and genomic characterization of Phormidium yuhuli AB48 isolated from an industrial photobioreactor environment. P. yuhuli AB48 is a filamentous phototactic cyanobacterium with a growth rate comparable to Synechocystis sp. PCC 6803. The isolate forms dense biofilms under high salinity and alkaline conditions and manifests a similar nutrient profile to Arthrospira platensis (Spirulina). We sequenced, assembled, and analyzed the P. yuhuli AB48 genome, the first closed circular isolate reference genome for a member of the Phormidium genus. We then used cultivation experiments in combination with proteomics and metabolomics to investigate growth characteristics and phenotypes related to industrial scale cultivation, including nitrogen and carbon utilization, salinity, and pH acclimation, as well as antibiotic resistance. These analyses provide insight into the biological mechanisms behind the desirable growth properties manifested by P. yuhuli AB48 and position it as a promising microbial cell factory for industrial-scale bioproduction[221, 1631].
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spelling pubmed-94209702022-08-30 The survivor strain: isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential Koch, Moritz Noonan, Avery J. C. Qiu, Yilin Dofher, Kalen Kieft, Brandon Mottahedeh, Soheyl Shastri, Manisha Hallam, Steven J. Front Bioeng Biotechnol Bioengineering and Biotechnology Despite their recognized potential, current applications of cyanobacteria as microbial cell factories remain in early stages of development. This is partly due to the fact that engineered strains are often difficult to grow at scale. This technical challenge contrasts with the dense and highly productive cyanobacteria populations thriving in many natural environments. It has been proposed that the selection of strains pre-adapted for growth in industrial photobioreactors could enable more productive cultivation outcomes. Here, we described the initial morphological, physiological, and genomic characterization of Phormidium yuhuli AB48 isolated from an industrial photobioreactor environment. P. yuhuli AB48 is a filamentous phototactic cyanobacterium with a growth rate comparable to Synechocystis sp. PCC 6803. The isolate forms dense biofilms under high salinity and alkaline conditions and manifests a similar nutrient profile to Arthrospira platensis (Spirulina). We sequenced, assembled, and analyzed the P. yuhuli AB48 genome, the first closed circular isolate reference genome for a member of the Phormidium genus. We then used cultivation experiments in combination with proteomics and metabolomics to investigate growth characteristics and phenotypes related to industrial scale cultivation, including nitrogen and carbon utilization, salinity, and pH acclimation, as well as antibiotic resistance. These analyses provide insight into the biological mechanisms behind the desirable growth properties manifested by P. yuhuli AB48 and position it as a promising microbial cell factory for industrial-scale bioproduction[221, 1631]. Frontiers Media S.A. 2022-08-15 /pmc/articles/PMC9420970/ /pubmed/36046667 http://dx.doi.org/10.3389/fbioe.2022.932695 Text en Copyright © 2022 Koch, Noonan, Qiu, Dofher, Kieft, Mottahedeh, Shastri and Hallam. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Koch, Moritz
Noonan, Avery J. C.
Qiu, Yilin
Dofher, Kalen
Kieft, Brandon
Mottahedeh, Soheyl
Shastri, Manisha
Hallam, Steven J.
The survivor strain: isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential
title The survivor strain: isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential
title_full The survivor strain: isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential
title_fullStr The survivor strain: isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential
title_full_unstemmed The survivor strain: isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential
title_short The survivor strain: isolation and characterization of Phormidium yuhuli AB48, a filamentous phototactic cyanobacterium with biotechnological potential
title_sort survivor strain: isolation and characterization of phormidium yuhuli ab48, a filamentous phototactic cyanobacterium with biotechnological potential
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420970/
https://www.ncbi.nlm.nih.gov/pubmed/36046667
http://dx.doi.org/10.3389/fbioe.2022.932695
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