Cargando…

Sex differences in the therapeutic effect of unaltered versus NFκB sensing IL-4 over-expressing mesenchymal stromal cells in a murine model of chronic inflammatory bone loss

Wear particles from joint arthroplasties induce chronic inflammation associated with prolonged upregulation of nuclear factor kappa-B (NF-κB) signaling in macrophages and osteoclasts, which leads to osteolysis and implant loosening. Mesenchymal stromal cell (MSC)-based therapy showed great potential...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Huaishuang, Kushioka, Junichi, Toya, Masakazu, Utsunomiya, Takeshi, Hirata, Hirohito, Huang, Ejun Elijah, Tsubosaka, Masanori, Gao, Qi, Li, Xueping, Teissier, Victoria, Zhang, Ning, Goodman, Stuart B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9421000/
https://www.ncbi.nlm.nih.gov/pubmed/36046680
http://dx.doi.org/10.3389/fbioe.2022.962114
_version_ 1784777496832507904
author Shen, Huaishuang
Kushioka, Junichi
Toya, Masakazu
Utsunomiya, Takeshi
Hirata, Hirohito
Huang, Ejun Elijah
Tsubosaka, Masanori
Gao, Qi
Li, Xueping
Teissier, Victoria
Zhang, Ning
Goodman, Stuart B.
author_facet Shen, Huaishuang
Kushioka, Junichi
Toya, Masakazu
Utsunomiya, Takeshi
Hirata, Hirohito
Huang, Ejun Elijah
Tsubosaka, Masanori
Gao, Qi
Li, Xueping
Teissier, Victoria
Zhang, Ning
Goodman, Stuart B.
author_sort Shen, Huaishuang
collection PubMed
description Wear particles from joint arthroplasties induce chronic inflammation associated with prolonged upregulation of nuclear factor kappa-B (NF-κB) signaling in macrophages and osteoclasts, which leads to osteolysis and implant loosening. Mesenchymal stromal cell (MSC)-based therapy showed great potential for immunomodulation and mitigation of osteolysis in vivo, especially in the chronic phase of inflammation. We previously generated genetically modified MSCs that secrete the anti-inflammatory cytokine interleukin 4 (IL-4) in response to NF-κB activation (NFκB-IL-4 MSCs). However, whether the impact of sexual difference in the internal environment can alter the therapeutic effects of IL-4 over-secreting MSCs that simultaneously mitigate prolonged inflammation and enhance bone formation remains unknown. This study investigated the therapeutic effects of unaltered MSCs versus NFκB-IL-4 MSCs in mitigating chronic inflammation and enhancing bone formation in male and female mice. The murine model was established by continuous infusion of polyethylene particles contaminated with lipopolysaccharide (cPE) into the medullary cavity of the distal femur for 6 weeks to induce chronic inflammation. Unaltered MSCs or NFκB-IL-4 MSCs were infused into the femoral intramedullary cavity in sex-matched groups beginning 3 weeks after primary surgery. Femurs were harvested at 6 weeks, and bone marrow density was measured with micro-computational tomography. Numbers of osteoclast-like cells, osteoblasts, and macrophages were evaluated with histochemical and immunofluorescence staining. cPE infusion resulted in severe bone loss at the surgery site, increased tartrate-resistant acid phosphatase positive osteoclasts and M1 pro-inflammatory macrophages, and decreased alkaline phosphatase expression. MSC-based therapy effectively decreased local bone loss and polarized M1 macrophages into an M2 anti-inflammatory phenotype. In females, unaltered MSCs demonstrated a larger impact in enhancing the osteogenesis, but they demonstrated similar anti-inflammatory effects compared to NFκB-IL-4 MSCs. These results demonstrated that local inflammatory bone loss can be effectively modulated via MSC-based treatments in a sexually dimorphic manner, which could be an efficacious therapeutic strategy for treatment of periprosthetic osteolysis in both genders.
format Online
Article
Text
id pubmed-9421000
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-94210002022-08-30 Sex differences in the therapeutic effect of unaltered versus NFκB sensing IL-4 over-expressing mesenchymal stromal cells in a murine model of chronic inflammatory bone loss Shen, Huaishuang Kushioka, Junichi Toya, Masakazu Utsunomiya, Takeshi Hirata, Hirohito Huang, Ejun Elijah Tsubosaka, Masanori Gao, Qi Li, Xueping Teissier, Victoria Zhang, Ning Goodman, Stuart B. Front Bioeng Biotechnol Bioengineering and Biotechnology Wear particles from joint arthroplasties induce chronic inflammation associated with prolonged upregulation of nuclear factor kappa-B (NF-κB) signaling in macrophages and osteoclasts, which leads to osteolysis and implant loosening. Mesenchymal stromal cell (MSC)-based therapy showed great potential for immunomodulation and mitigation of osteolysis in vivo, especially in the chronic phase of inflammation. We previously generated genetically modified MSCs that secrete the anti-inflammatory cytokine interleukin 4 (IL-4) in response to NF-κB activation (NFκB-IL-4 MSCs). However, whether the impact of sexual difference in the internal environment can alter the therapeutic effects of IL-4 over-secreting MSCs that simultaneously mitigate prolonged inflammation and enhance bone formation remains unknown. This study investigated the therapeutic effects of unaltered MSCs versus NFκB-IL-4 MSCs in mitigating chronic inflammation and enhancing bone formation in male and female mice. The murine model was established by continuous infusion of polyethylene particles contaminated with lipopolysaccharide (cPE) into the medullary cavity of the distal femur for 6 weeks to induce chronic inflammation. Unaltered MSCs or NFκB-IL-4 MSCs were infused into the femoral intramedullary cavity in sex-matched groups beginning 3 weeks after primary surgery. Femurs were harvested at 6 weeks, and bone marrow density was measured with micro-computational tomography. Numbers of osteoclast-like cells, osteoblasts, and macrophages were evaluated with histochemical and immunofluorescence staining. cPE infusion resulted in severe bone loss at the surgery site, increased tartrate-resistant acid phosphatase positive osteoclasts and M1 pro-inflammatory macrophages, and decreased alkaline phosphatase expression. MSC-based therapy effectively decreased local bone loss and polarized M1 macrophages into an M2 anti-inflammatory phenotype. In females, unaltered MSCs demonstrated a larger impact in enhancing the osteogenesis, but they demonstrated similar anti-inflammatory effects compared to NFκB-IL-4 MSCs. These results demonstrated that local inflammatory bone loss can be effectively modulated via MSC-based treatments in a sexually dimorphic manner, which could be an efficacious therapeutic strategy for treatment of periprosthetic osteolysis in both genders. Frontiers Media S.A. 2022-08-15 /pmc/articles/PMC9421000/ /pubmed/36046680 http://dx.doi.org/10.3389/fbioe.2022.962114 Text en Copyright © 2022 Shen, Kushioka, Toya, Utsunomiya, Hirata, Huang, Tsubosaka, Gao, Li, Teissier, Zhang and Goodman. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Shen, Huaishuang
Kushioka, Junichi
Toya, Masakazu
Utsunomiya, Takeshi
Hirata, Hirohito
Huang, Ejun Elijah
Tsubosaka, Masanori
Gao, Qi
Li, Xueping
Teissier, Victoria
Zhang, Ning
Goodman, Stuart B.
Sex differences in the therapeutic effect of unaltered versus NFκB sensing IL-4 over-expressing mesenchymal stromal cells in a murine model of chronic inflammatory bone loss
title Sex differences in the therapeutic effect of unaltered versus NFκB sensing IL-4 over-expressing mesenchymal stromal cells in a murine model of chronic inflammatory bone loss
title_full Sex differences in the therapeutic effect of unaltered versus NFκB sensing IL-4 over-expressing mesenchymal stromal cells in a murine model of chronic inflammatory bone loss
title_fullStr Sex differences in the therapeutic effect of unaltered versus NFκB sensing IL-4 over-expressing mesenchymal stromal cells in a murine model of chronic inflammatory bone loss
title_full_unstemmed Sex differences in the therapeutic effect of unaltered versus NFκB sensing IL-4 over-expressing mesenchymal stromal cells in a murine model of chronic inflammatory bone loss
title_short Sex differences in the therapeutic effect of unaltered versus NFκB sensing IL-4 over-expressing mesenchymal stromal cells in a murine model of chronic inflammatory bone loss
title_sort sex differences in the therapeutic effect of unaltered versus nfκb sensing il-4 over-expressing mesenchymal stromal cells in a murine model of chronic inflammatory bone loss
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9421000/
https://www.ncbi.nlm.nih.gov/pubmed/36046680
http://dx.doi.org/10.3389/fbioe.2022.962114
work_keys_str_mv AT shenhuaishuang sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT kushiokajunichi sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT toyamasakazu sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT utsunomiyatakeshi sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT hiratahirohito sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT huangejunelijah sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT tsubosakamasanori sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT gaoqi sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT lixueping sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT teissiervictoria sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT zhangning sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss
AT goodmanstuartb sexdifferencesinthetherapeuticeffectofunalteredversusnfkbsensingil4overexpressingmesenchymalstromalcellsinamurinemodelofchronicinflammatoryboneloss