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Detection of periodontal disease activity based on histatin degradation in individuals with cerebral palsy
OBJECTIVES: This proof-of-concept study aimed at evaluating the proteolytic profile of histatin 1 and 5 in saliva of adolescents with spastic cerebral palsy (CP) with gingivitis. METHODS: This cross-sectional study included 24 individuals allocated into three groups: G1 (CP with gingivitis; n = 8),...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9421316/ https://www.ncbi.nlm.nih.gov/pubmed/36046535 http://dx.doi.org/10.1016/j.heliyon.2022.e10134 |
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author | de Gutierrez, Gabriela M. Marin, Lina M. Xiao, Yizhi Escalante-Herrera, Andrea Santos, Maria T.B.R. Siqueira, Walter L. |
author_facet | de Gutierrez, Gabriela M. Marin, Lina M. Xiao, Yizhi Escalante-Herrera, Andrea Santos, Maria T.B.R. Siqueira, Walter L. |
author_sort | de Gutierrez, Gabriela M. |
collection | PubMed |
description | OBJECTIVES: This proof-of-concept study aimed at evaluating the proteolytic profile of histatin 1 and 5 in saliva of adolescents with spastic cerebral palsy (CP) with gingivitis. METHODS: This cross-sectional study included 24 individuals allocated into three groups: G1 (CP with gingivitis; n = 8), G2 (without CP and without gingivitis; n = 8), and G3 (without CP and with gingivitis; n = 8). The gingival index (GI) and simplified oral hygiene index (OHI–S) were evaluated. Whole saliva was collected and used to assess the rate and mode of histatin 1 and 5 at different times. The degradation products were visualized after cationic PAGE and the protein band densities (BDs) were compared with a protein standard. Fragmentation products were collected from the gel, pooled by group and characterized by mass spectrometry. BDs and gingival health parameters were analyzed by One-Way ANOVA or Kruskal Wallis tests, whereas poisson multilevel regression was used to the factors that influenced histatin degradation (α = 5%). RESULTS: Groups G1 and G3 differed significantly on OHI–S, visible biofilm, oral calculus and GI (p < 0.001). Poisson Regression showed that: 1) CP and gingivitis influenced the degradation of histatin 1 and 5 (p < 0.05); 2) The degradation of histatin 5 was influenced by age and male sex (p < 0.05); and 3) GI influenced significantly the degradation of histatin 1 (p < 0.001). Unique histatin degradation peptides were identified in individuals with gingivitis. CONSLUSIONS: These data demonstrated that both the kinetics and pattern of histatins degradation differ according to the gingival health or disease conditions. |
format | Online Article Text |
id | pubmed-9421316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-94213162022-08-30 Detection of periodontal disease activity based on histatin degradation in individuals with cerebral palsy de Gutierrez, Gabriela M. Marin, Lina M. Xiao, Yizhi Escalante-Herrera, Andrea Santos, Maria T.B.R. Siqueira, Walter L. Heliyon Research Article OBJECTIVES: This proof-of-concept study aimed at evaluating the proteolytic profile of histatin 1 and 5 in saliva of adolescents with spastic cerebral palsy (CP) with gingivitis. METHODS: This cross-sectional study included 24 individuals allocated into three groups: G1 (CP with gingivitis; n = 8), G2 (without CP and without gingivitis; n = 8), and G3 (without CP and with gingivitis; n = 8). The gingival index (GI) and simplified oral hygiene index (OHI–S) were evaluated. Whole saliva was collected and used to assess the rate and mode of histatin 1 and 5 at different times. The degradation products were visualized after cationic PAGE and the protein band densities (BDs) were compared with a protein standard. Fragmentation products were collected from the gel, pooled by group and characterized by mass spectrometry. BDs and gingival health parameters were analyzed by One-Way ANOVA or Kruskal Wallis tests, whereas poisson multilevel regression was used to the factors that influenced histatin degradation (α = 5%). RESULTS: Groups G1 and G3 differed significantly on OHI–S, visible biofilm, oral calculus and GI (p < 0.001). Poisson Regression showed that: 1) CP and gingivitis influenced the degradation of histatin 1 and 5 (p < 0.05); 2) The degradation of histatin 5 was influenced by age and male sex (p < 0.05); and 3) GI influenced significantly the degradation of histatin 1 (p < 0.001). Unique histatin degradation peptides were identified in individuals with gingivitis. CONSLUSIONS: These data demonstrated that both the kinetics and pattern of histatins degradation differ according to the gingival health or disease conditions. Elsevier 2022-08-13 /pmc/articles/PMC9421316/ /pubmed/36046535 http://dx.doi.org/10.1016/j.heliyon.2022.e10134 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article de Gutierrez, Gabriela M. Marin, Lina M. Xiao, Yizhi Escalante-Herrera, Andrea Santos, Maria T.B.R. Siqueira, Walter L. Detection of periodontal disease activity based on histatin degradation in individuals with cerebral palsy |
title | Detection of periodontal disease activity based on histatin degradation in individuals with cerebral palsy |
title_full | Detection of periodontal disease activity based on histatin degradation in individuals with cerebral palsy |
title_fullStr | Detection of periodontal disease activity based on histatin degradation in individuals with cerebral palsy |
title_full_unstemmed | Detection of periodontal disease activity based on histatin degradation in individuals with cerebral palsy |
title_short | Detection of periodontal disease activity based on histatin degradation in individuals with cerebral palsy |
title_sort | detection of periodontal disease activity based on histatin degradation in individuals with cerebral palsy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9421316/ https://www.ncbi.nlm.nih.gov/pubmed/36046535 http://dx.doi.org/10.1016/j.heliyon.2022.e10134 |
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