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Construction and validation of a novel ten miRNA-pair based signature for the prognosis of clear cell renal cell carcinoma

BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is the most predominate pathological subtype of renal cell carcinoma, causing a recurrence or metastasis rate as high as 20% to 40% after operation, for which effective prognostic signature is urgently needed. METHODS: The mRNA and miRNA profiles o...

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Autores principales: Wang, Yulin, Shen, Ziyan, Mo, Shaocong, Dai, Leijie, Song, Biao, Gu, Wenchao, Ding, Xiaoqiang, Zhang, Xiaoyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9421317/
https://www.ncbi.nlm.nih.gov/pubmed/35998436
http://dx.doi.org/10.1016/j.tranon.2022.101519
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author Wang, Yulin
Shen, Ziyan
Mo, Shaocong
Dai, Leijie
Song, Biao
Gu, Wenchao
Ding, Xiaoqiang
Zhang, Xiaoyan
author_facet Wang, Yulin
Shen, Ziyan
Mo, Shaocong
Dai, Leijie
Song, Biao
Gu, Wenchao
Ding, Xiaoqiang
Zhang, Xiaoyan
author_sort Wang, Yulin
collection PubMed
description BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is the most predominate pathological subtype of renal cell carcinoma, causing a recurrence or metastasis rate as high as 20% to 40% after operation, for which effective prognostic signature is urgently needed. METHODS: The mRNA and miRNA profiles of ccRCC specimens were collected from the Cancer Genome Atlas. MiRNA-pair risk score (miPRS) for each miRNA pair was generated as a signature and validated by univariate and multivariate Cox proportional hazards regression analysis. Functional enrichment was performed, and immune cells infiltration, as well as tumor mutation burden (TMB), and immunophenoscore (IPS) were evaluated between high and low miPRS groups. Target gene-prediction and differentially expressed gene-analysis were performed based on databases of miRDB, miRTarBase, and TargetScan. Multivariate Cox proportional hazards regression analysis was adopted to establish the prognostic model and Kaplan-Meier survival analysis was performed. FINDINGS: A novel 10 miRNA-pair based signature was established. Area under the time-dependent receiver operating curve proved the performance of the signature in the training, validation, and testing cohorts. Higher TMB, as well as the higher CTLA4-negative PD1-negative IPS, were discovered in high miPRS patients. A prognostic model was built based on miPRS (1 year-, 5 year-, 10 year- ROC-AUC=0.92, 0.84, 0.82, respectively). INTERPRETATION: The model based on miPRS is a novel and valid tool for predicting the prognosis of ccRCC. FUNDING: This study was supported by research grants from the China National Natural Scientific Foundation (81903972, 82002018, and 82170752) and Shanghai Sailing Program (19YF1406700 and 20YF1406000).
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spelling pubmed-94213172022-09-08 Construction and validation of a novel ten miRNA-pair based signature for the prognosis of clear cell renal cell carcinoma Wang, Yulin Shen, Ziyan Mo, Shaocong Dai, Leijie Song, Biao Gu, Wenchao Ding, Xiaoqiang Zhang, Xiaoyan Transl Oncol Original Research BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is the most predominate pathological subtype of renal cell carcinoma, causing a recurrence or metastasis rate as high as 20% to 40% after operation, for which effective prognostic signature is urgently needed. METHODS: The mRNA and miRNA profiles of ccRCC specimens were collected from the Cancer Genome Atlas. MiRNA-pair risk score (miPRS) for each miRNA pair was generated as a signature and validated by univariate and multivariate Cox proportional hazards regression analysis. Functional enrichment was performed, and immune cells infiltration, as well as tumor mutation burden (TMB), and immunophenoscore (IPS) were evaluated between high and low miPRS groups. Target gene-prediction and differentially expressed gene-analysis were performed based on databases of miRDB, miRTarBase, and TargetScan. Multivariate Cox proportional hazards regression analysis was adopted to establish the prognostic model and Kaplan-Meier survival analysis was performed. FINDINGS: A novel 10 miRNA-pair based signature was established. Area under the time-dependent receiver operating curve proved the performance of the signature in the training, validation, and testing cohorts. Higher TMB, as well as the higher CTLA4-negative PD1-negative IPS, were discovered in high miPRS patients. A prognostic model was built based on miPRS (1 year-, 5 year-, 10 year- ROC-AUC=0.92, 0.84, 0.82, respectively). INTERPRETATION: The model based on miPRS is a novel and valid tool for predicting the prognosis of ccRCC. FUNDING: This study was supported by research grants from the China National Natural Scientific Foundation (81903972, 82002018, and 82170752) and Shanghai Sailing Program (19YF1406700 and 20YF1406000). Neoplasia Press 2022-08-20 /pmc/articles/PMC9421317/ /pubmed/35998436 http://dx.doi.org/10.1016/j.tranon.2022.101519 Text en © 2022 Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research
Wang, Yulin
Shen, Ziyan
Mo, Shaocong
Dai, Leijie
Song, Biao
Gu, Wenchao
Ding, Xiaoqiang
Zhang, Xiaoyan
Construction and validation of a novel ten miRNA-pair based signature for the prognosis of clear cell renal cell carcinoma
title Construction and validation of a novel ten miRNA-pair based signature for the prognosis of clear cell renal cell carcinoma
title_full Construction and validation of a novel ten miRNA-pair based signature for the prognosis of clear cell renal cell carcinoma
title_fullStr Construction and validation of a novel ten miRNA-pair based signature for the prognosis of clear cell renal cell carcinoma
title_full_unstemmed Construction and validation of a novel ten miRNA-pair based signature for the prognosis of clear cell renal cell carcinoma
title_short Construction and validation of a novel ten miRNA-pair based signature for the prognosis of clear cell renal cell carcinoma
title_sort construction and validation of a novel ten mirna-pair based signature for the prognosis of clear cell renal cell carcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9421317/
https://www.ncbi.nlm.nih.gov/pubmed/35998436
http://dx.doi.org/10.1016/j.tranon.2022.101519
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