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An improved organotypic cell culture system to study tissue-resident macrophages ex vivo

Tissue-resident macrophages (TRMs) perform organ-specific functions that are dependent on factors such as hematopoietic origin, local environment, and biological influences. A diverse range of in vitro culture systems have been developed to decipher TRM functions, including bone marrow-derived macro...

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Detalles Bibliográficos
Autores principales: Aktories, Philipp, Petry, Philippe, Glatz, Paulo, Andrieux, Geoffroy, Oschwald, Alexander, Botterer, Hannah, Gorka, Oliver, Erny, Daniel, Boerries, Melanie, Henneke, Philipp, Groß, Olaf, Prinz, Marco, Kierdorf, Katrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9421540/
https://www.ncbi.nlm.nih.gov/pubmed/36046625
http://dx.doi.org/10.1016/j.crmeth.2022.100260
Descripción
Sumario:Tissue-resident macrophages (TRMs) perform organ-specific functions that are dependent on factors such as hematopoietic origin, local environment, and biological influences. A diverse range of in vitro culture systems have been developed to decipher TRM functions, including bone marrow-derived macrophages (BMDMs), induced pluripotent stem cell (iPSC)-derived TRMs, or immortalized cell lines. However, despite the usefulness of such systems, there are notable limitations. Attempts to culture primary macrophages often require purification of cells and lack a high cell yield and consistent phenotype. Here, we aimed to address these limitations by establishing an organotypic primary cell culture protocol. We obtained long-term monocultures of macrophages derived from distinct organs without prior purification using specific growth factors and tissue normoxic conditions that largely conserved a TRM-like identity in vitro. Thus, this organotypic system offers an ideal screening platform for primary macrophages from different organs that can be used for a wide range of assays and readouts.