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Tear Film Stability as a Function of Tunable Mucin Concentration Attached to Supported Lipid Bilayers

[Image: see text] In this work, we describe the development of a tunable, acellular in vitro model of the mucin layer of the human tear film. First, supported lipid bilayers (SLBs) comprised of the phospholipid DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) and biotinyl cap PE (1,2-dioleoyl-sn-glyc...

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Autores principales: Cui, Kiara W., Myung, David J., Fuller, Gerald G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9421887/
https://www.ncbi.nlm.nih.gov/pubmed/35972346
http://dx.doi.org/10.1021/acs.jpcb.2c04154
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author Cui, Kiara W.
Myung, David J.
Fuller, Gerald G.
author_facet Cui, Kiara W.
Myung, David J.
Fuller, Gerald G.
author_sort Cui, Kiara W.
collection PubMed
description [Image: see text] In this work, we describe the development of a tunable, acellular in vitro model of the mucin layer of the human tear film. First, supported lipid bilayers (SLBs) comprised of the phospholipid DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) and biotinyl cap PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl)) are created on the surface of a glass dome with radius of curvature comparable to the human eye. Next, biotinylated bovine submaxillary mucins (BSM) are tethered onto the SLB using streptavidin protein. The mucin presentation can be tuned by altering the concentration of biotinylated BSM, which we confirm using fluorescence microscopy. Due to the optically smooth surface that results, this model is compatible with interferometry for monitoring film thickness. Below a certain level of mucin coverage, we observe short model tear film breakup times, mimicking a deficiency in membrane-associated mucins. In contrast, the breakup time is significantly delayed for SLBs with high mucin coverage. Because no differences in mobility or wettability were observed, we hypothesize that higher mucin coverage provides a thicker hydrated layer that can protect against external disturbances to thin film stability. This advance paves the way for a more physiological, interferometry-based in vitro model for investigating tear film breakup.
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spelling pubmed-94218872022-08-30 Tear Film Stability as a Function of Tunable Mucin Concentration Attached to Supported Lipid Bilayers Cui, Kiara W. Myung, David J. Fuller, Gerald G. J Phys Chem B [Image: see text] In this work, we describe the development of a tunable, acellular in vitro model of the mucin layer of the human tear film. First, supported lipid bilayers (SLBs) comprised of the phospholipid DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) and biotinyl cap PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl)) are created on the surface of a glass dome with radius of curvature comparable to the human eye. Next, biotinylated bovine submaxillary mucins (BSM) are tethered onto the SLB using streptavidin protein. The mucin presentation can be tuned by altering the concentration of biotinylated BSM, which we confirm using fluorescence microscopy. Due to the optically smooth surface that results, this model is compatible with interferometry for monitoring film thickness. Below a certain level of mucin coverage, we observe short model tear film breakup times, mimicking a deficiency in membrane-associated mucins. In contrast, the breakup time is significantly delayed for SLBs with high mucin coverage. Because no differences in mobility or wettability were observed, we hypothesize that higher mucin coverage provides a thicker hydrated layer that can protect against external disturbances to thin film stability. This advance paves the way for a more physiological, interferometry-based in vitro model for investigating tear film breakup. American Chemical Society 2022-08-16 2022-08-25 /pmc/articles/PMC9421887/ /pubmed/35972346 http://dx.doi.org/10.1021/acs.jpcb.2c04154 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Cui, Kiara W.
Myung, David J.
Fuller, Gerald G.
Tear Film Stability as a Function of Tunable Mucin Concentration Attached to Supported Lipid Bilayers
title Tear Film Stability as a Function of Tunable Mucin Concentration Attached to Supported Lipid Bilayers
title_full Tear Film Stability as a Function of Tunable Mucin Concentration Attached to Supported Lipid Bilayers
title_fullStr Tear Film Stability as a Function of Tunable Mucin Concentration Attached to Supported Lipid Bilayers
title_full_unstemmed Tear Film Stability as a Function of Tunable Mucin Concentration Attached to Supported Lipid Bilayers
title_short Tear Film Stability as a Function of Tunable Mucin Concentration Attached to Supported Lipid Bilayers
title_sort tear film stability as a function of tunable mucin concentration attached to supported lipid bilayers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9421887/
https://www.ncbi.nlm.nih.gov/pubmed/35972346
http://dx.doi.org/10.1021/acs.jpcb.2c04154
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