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Interferon-α action in cytokine profile in eosinophilic nasal polyp cultures

INTRODUCTION: Chronic rhinosinusitis is currently classified into two types: chronic rhinosinusitis without nasal polyps and chronic rhinosinusitis with nasal polyps. In the West, approximately 80% of chronic rhinosinusitis with nasal polyps cases are characterized by a predominantly eosinophilic ce...

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Autores principales: Sousa, Júlio Cláudio, Etchbehere, Renata Margarida, Alves, Eduardo Arthur Rodovalho, Stark, Letícia Montes, Murta, Eddie Fernando Cândido, Michelin, Márcia Antoniazi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9422409/
https://www.ncbi.nlm.nih.gov/pubmed/31870738
http://dx.doi.org/10.1016/j.bjorl.2019.08.010
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author Sousa, Júlio Cláudio
Etchbehere, Renata Margarida
Alves, Eduardo Arthur Rodovalho
Stark, Letícia Montes
Murta, Eddie Fernando Cândido
Michelin, Márcia Antoniazi
author_facet Sousa, Júlio Cláudio
Etchbehere, Renata Margarida
Alves, Eduardo Arthur Rodovalho
Stark, Letícia Montes
Murta, Eddie Fernando Cândido
Michelin, Márcia Antoniazi
author_sort Sousa, Júlio Cláudio
collection PubMed
description INTRODUCTION: Chronic rhinosinusitis is currently classified into two types: chronic rhinosinusitis without nasal polyps and chronic rhinosinusitis with nasal polyps. In the West, approximately 80% of chronic rhinosinusitis with nasal polyps cases are characterized by a predominantly eosinophilic cell infiltrate and a Th2 cytokine pattern. OBJECTIVE: To evaluate the effect of Interferon-α on cytokine levels of the eosinophilic nasal polyp cell culture supernatant. METHODS: Cell cultures were performed based on nasal polypoid tissue samples collected from 13 patients with eosinophilic chronic rhinosinusitis with nasal polyps. Polyps were considered eosinophilic according to the histopathological examination. Cell cultures were stimulated with 3000 IU of interferon-α. Before and after the stimulus, concentrations of Interferon-γ, tumor necrosis factor αand IL 2, 4, 6 and 10, using cytometric bead array, were assessed. RESULTS: Cell samples from eosinophilic nasal polyps from 13 patients were included in the study. Twenty-four hours after interferon-α stimulation, eosinophilic nasal polyp culture supernatants showed significantly decreased IL-4 concentrations and increase in interferon-γ, IL-10 and IL-6 concentrations compared to controls. There were no significant differences in tumor necrosis factor -α and IL-2 concentrations. CONCLUSION: We demonstrated that interferon-α in vitro alters the pattern of cytokines in cell cultures of eosinophilic nasal polyps. Analysis of these alterations suggests that interferon-α promotes a rebalancing of inflammatory profiles in cell cultures, favoring the expression of Th1 and regulatory cytokines over Th2 cytokines.
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spelling pubmed-94224092022-08-31 Interferon-α action in cytokine profile in eosinophilic nasal polyp cultures Sousa, Júlio Cláudio Etchbehere, Renata Margarida Alves, Eduardo Arthur Rodovalho Stark, Letícia Montes Murta, Eddie Fernando Cândido Michelin, Márcia Antoniazi Braz J Otorhinolaryngol Original Article INTRODUCTION: Chronic rhinosinusitis is currently classified into two types: chronic rhinosinusitis without nasal polyps and chronic rhinosinusitis with nasal polyps. In the West, approximately 80% of chronic rhinosinusitis with nasal polyps cases are characterized by a predominantly eosinophilic cell infiltrate and a Th2 cytokine pattern. OBJECTIVE: To evaluate the effect of Interferon-α on cytokine levels of the eosinophilic nasal polyp cell culture supernatant. METHODS: Cell cultures were performed based on nasal polypoid tissue samples collected from 13 patients with eosinophilic chronic rhinosinusitis with nasal polyps. Polyps were considered eosinophilic according to the histopathological examination. Cell cultures were stimulated with 3000 IU of interferon-α. Before and after the stimulus, concentrations of Interferon-γ, tumor necrosis factor αand IL 2, 4, 6 and 10, using cytometric bead array, were assessed. RESULTS: Cell samples from eosinophilic nasal polyps from 13 patients were included in the study. Twenty-four hours after interferon-α stimulation, eosinophilic nasal polyp culture supernatants showed significantly decreased IL-4 concentrations and increase in interferon-γ, IL-10 and IL-6 concentrations compared to controls. There were no significant differences in tumor necrosis factor -α and IL-2 concentrations. CONCLUSION: We demonstrated that interferon-α in vitro alters the pattern of cytokines in cell cultures of eosinophilic nasal polyps. Analysis of these alterations suggests that interferon-α promotes a rebalancing of inflammatory profiles in cell cultures, favoring the expression of Th1 and regulatory cytokines over Th2 cytokines. Elsevier 2019-11-02 /pmc/articles/PMC9422409/ /pubmed/31870738 http://dx.doi.org/10.1016/j.bjorl.2019.08.010 Text en © 2019 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Published by Elsevier Editora Ltda. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Sousa, Júlio Cláudio
Etchbehere, Renata Margarida
Alves, Eduardo Arthur Rodovalho
Stark, Letícia Montes
Murta, Eddie Fernando Cândido
Michelin, Márcia Antoniazi
Interferon-α action in cytokine profile in eosinophilic nasal polyp cultures
title Interferon-α action in cytokine profile in eosinophilic nasal polyp cultures
title_full Interferon-α action in cytokine profile in eosinophilic nasal polyp cultures
title_fullStr Interferon-α action in cytokine profile in eosinophilic nasal polyp cultures
title_full_unstemmed Interferon-α action in cytokine profile in eosinophilic nasal polyp cultures
title_short Interferon-α action in cytokine profile in eosinophilic nasal polyp cultures
title_sort interferon-α action in cytokine profile in eosinophilic nasal polyp cultures
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9422409/
https://www.ncbi.nlm.nih.gov/pubmed/31870738
http://dx.doi.org/10.1016/j.bjorl.2019.08.010
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