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Nasal lavage cytology and mucosal histopathological alterations in patients with rhinitis()
INTRODUCTION: The extent of epithelial lesion in allergic and non-allergic rhinitis and its association with inflammatory changes in nasal lavage has not been clarified. OBJECTIVE: To verify the association between the inflammatory cells in the nasal lavage, epithelial lesion extent and basement mem...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9422499/ https://www.ncbi.nlm.nih.gov/pubmed/30846420 http://dx.doi.org/10.1016/j.bjorl.2019.01.005 |
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author | Kovalhuk, Loreni C.S. Telles, Ederaldo Queiroz Lima, Monica Nunes Rosario Filho, Nelson A. |
author_facet | Kovalhuk, Loreni C.S. Telles, Ederaldo Queiroz Lima, Monica Nunes Rosario Filho, Nelson A. |
author_sort | Kovalhuk, Loreni C.S. |
collection | PubMed |
description | INTRODUCTION: The extent of epithelial lesion in allergic and non-allergic rhinitis and its association with inflammatory changes in nasal lavage has not been clarified. OBJECTIVE: To verify the association between the inflammatory cells in the nasal lavage, epithelial lesion extent and basement membrane thickness, in the nasal mucosa of patients with rhinitis; to determine the cutoff point of the percentage of eosinophils in the nasal lavage associated with the atopic patients. METHODS: Patients with rhinitis and indication for septoplasty and (or) turbinectomy for turbinate hypertrophy were selected, and were submitted to allergy skin tests, nasal lavage with measurement of albumin and interleukin-8 levels, total and differential counting of cells, and mucosal histopathological analysis to determine the extent of epithelial lesion, and degree of basement membrane thickening. RESULTS: Fifty-six patients with a median age of 24.5 years and a diagnosis of allergic rhinitis (n = 36) and non-allergic rhinitis (n = 20) were studied. In atopic subjects, allergy skin tests were positive for Dermatophagoides pteronyssinus in 35 (97.0%) and Lolium perenne in 18 (50.0%). Atopic subjects showed a higher clinical score index of rhinitis compared to non-atopic ones. The total count of cells, neutrophils, and levels of albumin and IL-8 were not different in the nasal lavage of atopic and non-atopic subjects. The cutoff point for eosinophil count in nasal fluid for the distinction between allergic rhinitis and non-allergic rhinitis was 4%. Some degree of epithelial lesion was more frequent in allergic rhinitis (94%) than in non-allergic rhinitis (65%) patients. In the presence of basement membrane thickness, as a marker of remodeling, there was no difference in the nasal lavage of patients with allergic rhinitis and non-allergic rhinitis. CONCLUSION: In this series, 4% was the cutoff point for the number of eosinophils in the nasal lavage, for atopy differentiation. Upper airway remodeling accessed by basement membrane thickness showed similar inflammatory cell infiltrate in the nasal lavage, regardless of the presence of atopy. |
format | Online Article Text |
id | pubmed-9422499 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-94224992022-08-31 Nasal lavage cytology and mucosal histopathological alterations in patients with rhinitis() Kovalhuk, Loreni C.S. Telles, Ederaldo Queiroz Lima, Monica Nunes Rosario Filho, Nelson A. Braz J Otorhinolaryngol Original Article INTRODUCTION: The extent of epithelial lesion in allergic and non-allergic rhinitis and its association with inflammatory changes in nasal lavage has not been clarified. OBJECTIVE: To verify the association between the inflammatory cells in the nasal lavage, epithelial lesion extent and basement membrane thickness, in the nasal mucosa of patients with rhinitis; to determine the cutoff point of the percentage of eosinophils in the nasal lavage associated with the atopic patients. METHODS: Patients with rhinitis and indication for septoplasty and (or) turbinectomy for turbinate hypertrophy were selected, and were submitted to allergy skin tests, nasal lavage with measurement of albumin and interleukin-8 levels, total and differential counting of cells, and mucosal histopathological analysis to determine the extent of epithelial lesion, and degree of basement membrane thickening. RESULTS: Fifty-six patients with a median age of 24.5 years and a diagnosis of allergic rhinitis (n = 36) and non-allergic rhinitis (n = 20) were studied. In atopic subjects, allergy skin tests were positive for Dermatophagoides pteronyssinus in 35 (97.0%) and Lolium perenne in 18 (50.0%). Atopic subjects showed a higher clinical score index of rhinitis compared to non-atopic ones. The total count of cells, neutrophils, and levels of albumin and IL-8 were not different in the nasal lavage of atopic and non-atopic subjects. The cutoff point for eosinophil count in nasal fluid for the distinction between allergic rhinitis and non-allergic rhinitis was 4%. Some degree of epithelial lesion was more frequent in allergic rhinitis (94%) than in non-allergic rhinitis (65%) patients. In the presence of basement membrane thickness, as a marker of remodeling, there was no difference in the nasal lavage of patients with allergic rhinitis and non-allergic rhinitis. CONCLUSION: In this series, 4% was the cutoff point for the number of eosinophils in the nasal lavage, for atopy differentiation. Upper airway remodeling accessed by basement membrane thickness showed similar inflammatory cell infiltrate in the nasal lavage, regardless of the presence of atopy. Elsevier 2019-02-22 /pmc/articles/PMC9422499/ /pubmed/30846420 http://dx.doi.org/10.1016/j.bjorl.2019.01.005 Text en © 2019 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Published by Elsevier Editora Ltda. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Original Article Kovalhuk, Loreni C.S. Telles, Ederaldo Queiroz Lima, Monica Nunes Rosario Filho, Nelson A. Nasal lavage cytology and mucosal histopathological alterations in patients with rhinitis() |
title | Nasal lavage cytology and mucosal histopathological alterations in patients with rhinitis() |
title_full | Nasal lavage cytology and mucosal histopathological alterations in patients with rhinitis() |
title_fullStr | Nasal lavage cytology and mucosal histopathological alterations in patients with rhinitis() |
title_full_unstemmed | Nasal lavage cytology and mucosal histopathological alterations in patients with rhinitis() |
title_short | Nasal lavage cytology and mucosal histopathological alterations in patients with rhinitis() |
title_sort | nasal lavage cytology and mucosal histopathological alterations in patients with rhinitis() |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9422499/ https://www.ncbi.nlm.nih.gov/pubmed/30846420 http://dx.doi.org/10.1016/j.bjorl.2019.01.005 |
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