Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice

Vaccination route dictates the quality and localization of immune responses within tissues. Intranasal vaccination seeds tissue-resident adaptive immunity, alongside trained innate responses within the lung/airways, critical for superior protection against SARS-CoV-2. This protocol encompasses intra...

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Autores principales: D’Agostino, Michael R., Afkhami, Sam, Kang, Alisha, Marzok, Art, Miller, Matthew S., Xing, Zhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423002/
https://www.ncbi.nlm.nih.gov/pubmed/36065292
http://dx.doi.org/10.1016/j.xpro.2022.101652
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author D’Agostino, Michael R.
Afkhami, Sam
Kang, Alisha
Marzok, Art
Miller, Matthew S.
Xing, Zhou
author_facet D’Agostino, Michael R.
Afkhami, Sam
Kang, Alisha
Marzok, Art
Miller, Matthew S.
Xing, Zhou
author_sort D’Agostino, Michael R.
collection PubMed
description Vaccination route dictates the quality and localization of immune responses within tissues. Intranasal vaccination seeds tissue-resident adaptive immunity, alongside trained innate responses within the lung/airways, critical for superior protection against SARS-CoV-2. This protocol encompasses intranasal vaccination in mice, step-by-step bronchoalveolar lavage for both cellular and acellular airway components, lung mononuclear cell isolation, and detailed flow cytometric characterization of lung tissue-resident memory T cell responses, and airway macrophage-trained innate immunity. For complete details on the use and execution of this protocol, please refer to Afkhami et al. (2022).
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spelling pubmed-94230022022-08-30 Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice D’Agostino, Michael R. Afkhami, Sam Kang, Alisha Marzok, Art Miller, Matthew S. Xing, Zhou STAR Protoc Protocol Vaccination route dictates the quality and localization of immune responses within tissues. Intranasal vaccination seeds tissue-resident adaptive immunity, alongside trained innate responses within the lung/airways, critical for superior protection against SARS-CoV-2. This protocol encompasses intranasal vaccination in mice, step-by-step bronchoalveolar lavage for both cellular and acellular airway components, lung mononuclear cell isolation, and detailed flow cytometric characterization of lung tissue-resident memory T cell responses, and airway macrophage-trained innate immunity. For complete details on the use and execution of this protocol, please refer to Afkhami et al. (2022). Elsevier 2022-08-29 /pmc/articles/PMC9423002/ /pubmed/36065292 http://dx.doi.org/10.1016/j.xpro.2022.101652 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
D’Agostino, Michael R.
Afkhami, Sam
Kang, Alisha
Marzok, Art
Miller, Matthew S.
Xing, Zhou
Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice
title Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice
title_full Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice
title_fullStr Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice
title_full_unstemmed Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice
title_short Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice
title_sort protocol for isolation and characterization of lung tissue resident memory t cells and airway trained innate immunity after intranasal vaccination in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423002/
https://www.ncbi.nlm.nih.gov/pubmed/36065292
http://dx.doi.org/10.1016/j.xpro.2022.101652
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