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High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart

Mitochondrial respirometry is key to understand how environmental factors model energetic cellular process. In the case of ectotherms, thermal tolerance has been hypothesized to be intimately linked with mitochondria capability to produce enough adenosine triphosphate (ATP) to respond to the energet...

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Autores principales: Meza-Buendia, Ana Karen, Aparicio-Trejo, Omar Emiliano, Díaz, Fernando, Caamal-Monsreal, Claudia, Pedraza-Chaverri, José, Álvarez-Delgado, Carolina, Paschke, Kurt, Rosas, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423623/
https://www.ncbi.nlm.nih.gov/pubmed/36037204
http://dx.doi.org/10.1371/journal.pone.0273554
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author Meza-Buendia, Ana Karen
Aparicio-Trejo, Omar Emiliano
Díaz, Fernando
Caamal-Monsreal, Claudia
Pedraza-Chaverri, José
Álvarez-Delgado, Carolina
Paschke, Kurt
Rosas, Carlos
author_facet Meza-Buendia, Ana Karen
Aparicio-Trejo, Omar Emiliano
Díaz, Fernando
Caamal-Monsreal, Claudia
Pedraza-Chaverri, José
Álvarez-Delgado, Carolina
Paschke, Kurt
Rosas, Carlos
author_sort Meza-Buendia, Ana Karen
collection PubMed
description Mitochondrial respirometry is key to understand how environmental factors model energetic cellular process. In the case of ectotherms, thermal tolerance has been hypothesized to be intimately linked with mitochondria capability to produce enough adenosine triphosphate (ATP) to respond to the energetic demands of animals in high temperatures. In a recent study made in Octopus maya was proposed the hypothesis postulating that high temperatures could restrain female reproduction due to the limited capacity of the animals’ heart to sustain oxygen flow to the body, affecting in this manner energy production in the rest of the organs, including the ovarium Meza-Buendia AK et al. (2021). Unfortunately, until now, no reports have shown temperature effects and other environmental variables on cephalopod mitochondria activity because of the lack of a method to evaluate mitochondrial respiratory parameters in those species’ groups. In this sense and for the first time, this study developed a method to obtain mitochondrial respirometry data of adult Octopus maya’s heart. This protocol illustrates a step-by-step procedure to get high yield and functional mitochondria of cephalopod heart and procedure for determining the corresponding respiratory parameters. The procedure described in this paper takes approximately 3 to 4 hours from isolation of intact mitochondria to measurement of mitochondrial oxygen consumption.
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spelling pubmed-94236232022-08-30 High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart Meza-Buendia, Ana Karen Aparicio-Trejo, Omar Emiliano Díaz, Fernando Caamal-Monsreal, Claudia Pedraza-Chaverri, José Álvarez-Delgado, Carolina Paschke, Kurt Rosas, Carlos PLoS One Lab Protocol Mitochondrial respirometry is key to understand how environmental factors model energetic cellular process. In the case of ectotherms, thermal tolerance has been hypothesized to be intimately linked with mitochondria capability to produce enough adenosine triphosphate (ATP) to respond to the energetic demands of animals in high temperatures. In a recent study made in Octopus maya was proposed the hypothesis postulating that high temperatures could restrain female reproduction due to the limited capacity of the animals’ heart to sustain oxygen flow to the body, affecting in this manner energy production in the rest of the organs, including the ovarium Meza-Buendia AK et al. (2021). Unfortunately, until now, no reports have shown temperature effects and other environmental variables on cephalopod mitochondria activity because of the lack of a method to evaluate mitochondrial respiratory parameters in those species’ groups. In this sense and for the first time, this study developed a method to obtain mitochondrial respirometry data of adult Octopus maya’s heart. This protocol illustrates a step-by-step procedure to get high yield and functional mitochondria of cephalopod heart and procedure for determining the corresponding respiratory parameters. The procedure described in this paper takes approximately 3 to 4 hours from isolation of intact mitochondria to measurement of mitochondrial oxygen consumption. Public Library of Science 2022-08-29 /pmc/articles/PMC9423623/ /pubmed/36037204 http://dx.doi.org/10.1371/journal.pone.0273554 Text en © 2022 Meza-Buendia et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Lab Protocol
Meza-Buendia, Ana Karen
Aparicio-Trejo, Omar Emiliano
Díaz, Fernando
Caamal-Monsreal, Claudia
Pedraza-Chaverri, José
Álvarez-Delgado, Carolina
Paschke, Kurt
Rosas, Carlos
High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart
title High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart
title_full High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart
title_fullStr High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart
title_full_unstemmed High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart
title_short High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart
title_sort high resolution respirometry of isolated mitochondria from adult octopus maya (class: cephalopoda) systemic heart
topic Lab Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423623/
https://www.ncbi.nlm.nih.gov/pubmed/36037204
http://dx.doi.org/10.1371/journal.pone.0273554
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