Cargando…
Interleukin-25 enhances humoral immune responses caused by the rabies virus
Rabies is an important zoonotic disease caused by the rabies virus (RABV). Currently, no effective treatment is available for this condition. The prevention and control of rabies mainly depend on effective vaccination. Therefore, it is crucial to enhance the immune responses induced by the rabies va...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423819/ https://www.ncbi.nlm.nih.gov/pubmed/35999776 http://dx.doi.org/10.1080/21505594.2022.2116146 |
_version_ | 1784778099641024512 |
---|---|
author | Zhang, Yue Zhang, Mengwei Liao, Xilan Yu, Yunsong Liu, Qing Luo, Yongwen Luo, Jun Guo, Xiaofeng |
author_facet | Zhang, Yue Zhang, Mengwei Liao, Xilan Yu, Yunsong Liu, Qing Luo, Yongwen Luo, Jun Guo, Xiaofeng |
author_sort | Zhang, Yue |
collection | PubMed |
description | Rabies is an important zoonotic disease caused by the rabies virus (RABV). Currently, no effective treatment is available for this condition. The prevention and control of rabies mainly depend on effective vaccination. Therefore, it is crucial to enhance the immune responses induced by the rabies vaccine. Virus neutralizing antibodies (VNA) induced by rabies vaccines are important for the clearance of RABV. Interleukin-25 (IL-25) has been demonstrated to activate T helper type 2 cells that contribute to humoral immune responses. The IL-25 gene was inserted into the genome of RABV, and the immunogenicity of recombinant RABV with IL-25 gene was investigated to develop more efficient rabies vaccines. Here, we found that the expression of IL-25 did not affect RABV production in vitro and pathogenicity in vivo. However, recombinant RABV expression of IL-25 induced a better VNA level than the parental virus in mice. In addition, expression of IL-25 enhanced the IgG1 level induced by RABV. Furthermore, mice immunized with recombinant RABV showed a higher survival rate and milder clinical signs than those immunized with the parent strain after challenge with CVS-11. Thus, these results showed that IL-25 could enhance the humoral immune responses induced by RABV, suggesting that IL-25 can be used as a viral vaccine adjuvant. |
format | Online Article Text |
id | pubmed-9423819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-94238192022-08-30 Interleukin-25 enhances humoral immune responses caused by the rabies virus Zhang, Yue Zhang, Mengwei Liao, Xilan Yu, Yunsong Liu, Qing Luo, Yongwen Luo, Jun Guo, Xiaofeng Virulence Research Paper Rabies is an important zoonotic disease caused by the rabies virus (RABV). Currently, no effective treatment is available for this condition. The prevention and control of rabies mainly depend on effective vaccination. Therefore, it is crucial to enhance the immune responses induced by the rabies vaccine. Virus neutralizing antibodies (VNA) induced by rabies vaccines are important for the clearance of RABV. Interleukin-25 (IL-25) has been demonstrated to activate T helper type 2 cells that contribute to humoral immune responses. The IL-25 gene was inserted into the genome of RABV, and the immunogenicity of recombinant RABV with IL-25 gene was investigated to develop more efficient rabies vaccines. Here, we found that the expression of IL-25 did not affect RABV production in vitro and pathogenicity in vivo. However, recombinant RABV expression of IL-25 induced a better VNA level than the parental virus in mice. In addition, expression of IL-25 enhanced the IgG1 level induced by RABV. Furthermore, mice immunized with recombinant RABV showed a higher survival rate and milder clinical signs than those immunized with the parent strain after challenge with CVS-11. Thus, these results showed that IL-25 could enhance the humoral immune responses induced by RABV, suggesting that IL-25 can be used as a viral vaccine adjuvant. Taylor & Francis 2022-08-26 /pmc/articles/PMC9423819/ /pubmed/35999776 http://dx.doi.org/10.1080/21505594.2022.2116146 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Zhang, Yue Zhang, Mengwei Liao, Xilan Yu, Yunsong Liu, Qing Luo, Yongwen Luo, Jun Guo, Xiaofeng Interleukin-25 enhances humoral immune responses caused by the rabies virus |
title | Interleukin-25 enhances humoral immune responses caused by the rabies virus |
title_full | Interleukin-25 enhances humoral immune responses caused by the rabies virus |
title_fullStr | Interleukin-25 enhances humoral immune responses caused by the rabies virus |
title_full_unstemmed | Interleukin-25 enhances humoral immune responses caused by the rabies virus |
title_short | Interleukin-25 enhances humoral immune responses caused by the rabies virus |
title_sort | interleukin-25 enhances humoral immune responses caused by the rabies virus |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423819/ https://www.ncbi.nlm.nih.gov/pubmed/35999776 http://dx.doi.org/10.1080/21505594.2022.2116146 |
work_keys_str_mv | AT zhangyue interleukin25enhanceshumoralimmuneresponsescausedbytherabiesvirus AT zhangmengwei interleukin25enhanceshumoralimmuneresponsescausedbytherabiesvirus AT liaoxilan interleukin25enhanceshumoralimmuneresponsescausedbytherabiesvirus AT yuyunsong interleukin25enhanceshumoralimmuneresponsescausedbytherabiesvirus AT liuqing interleukin25enhanceshumoralimmuneresponsescausedbytherabiesvirus AT luoyongwen interleukin25enhanceshumoralimmuneresponsescausedbytherabiesvirus AT luojun interleukin25enhanceshumoralimmuneresponsescausedbytherabiesvirus AT guoxiaofeng interleukin25enhanceshumoralimmuneresponsescausedbytherabiesvirus |